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01-10-2017 | Original Paper

An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide

Authors: Madan Kumar Paudel, Seiichi Sakamoto, Hiroyuki Tanaka, Satoshi Morimoto

Published in: Journal of Natural Medicines | Issue 4/2017

Abstract

Wogonin glucuronide (wogonin 7-O-β-d-glucuronide, Wgn) is widely recognized as a constituent of Scutellariae radix, which is used in Kampo medicines. Wgn has been used for both pharmacological (antifebrile uses and in detoxification) and research purposes. A recombinant antigen-binding fragment (rFab) and an antigen-binding fragment from a monoclonal antibody (mFab) against Wgn were constructed and used in an indirect competitive enzyme-linked immunosorbent assay (icELISA) in this study. The rFab and mFab against Wgn showed both activity and recognition against Wgn. The developed icELISA was validated as a quantitative analytical method to detect Wgn by testing both its utility and its reliability using multiple concentrations of Wgn from S. radix. This approach provides a more economic method to analyze and purify Kampo medicines.

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Title: An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide
Authors: Madan Kumar Paudel
Seiichi Sakamoto
Hiroyuki Tanaka
Satoshi Morimoto
Publication date: 01-10-2017
Publisher: Springer Singapore
Published in: Journal of Natural Medicines / Issue 4/2017
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-017-1100-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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