Top

Journal of Assisted Reproduction and Genetics

Published in:

01-09-2013 | Gamete Biology

Retrieval and in vitro maturation of human oocytes from ovaries removed during surgery for endometrial carcinoma: a novel strategy for human oocyte research

Authors: Hiromitsu Shirasawa, Jin Kumagai, Wataru Sato, Yukiyo Kumazawa, Naoki Sato, Yukihiro Terada

Published in: Journal of Assisted Reproduction and Genetics | Issue 9/2013

Abstract

Purpose

To collect human oocytes from ovaries removed as part of surgical treatment for endometrial carcinoma, and to induce in vitro maturation of such oocytes to obtain material for research on human ovarian aging.

Methods

Design: Prospective clinical study. Setting: University Hospital. Patients: Eight patients aged 35–44 years with a preoperative diagnosis of Stage I endometrial cancer agreed to participate in this project. Interventions: Surgically removed ovaries were punctured; oocytes were collected from follicular fluid and matured in vitro. Immunofluorescent detection of microtubules and DNA labeling were performed after in vitro maturation. Main Outcome Measures: Number of oocytes collected and their in vitro maturation stage.

Results

In total, 87 oocytes were collected, 11 of which had completed metaphase II. Of the oocytes collected, 75 % were from three patients in their 30s, while the remaining 25 % were from five patients in their 40s. Several stages of oocytes were collected and the detection of microtubule arrangement and chromatin in various stages using fluorescence was possible.

Conclusion

Material for research on human ovarian aging can be obtained from ovaries removed during surgery for endometrial cancer.

Battaglia DE, Goodwin P, Klein NA, Soules MR. Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women. Hum Reprod (Oxford, England). 1996;11(10):2217–22.CrossRef

Chiang T, Duncan FE, Schindler K, Schultz RM, Lampson MA. Evidence that weakened centromere cohesion is a leading cause of age-related aneuploidy in oocytes. Curr Biol: CB. 2010;20(17):1522–8. doi:10.1016/j.cub.2010.06.069.PubMedCrossRef

Hasegawa H. The best approach to observe microtubule organization in oocyte cytoplasm production of oocyte wole mount samples by Buffer M Fixation. J Mamm Ova Res. 2009;26(4):232–3. doi:10.1274/jmor.26.232.CrossRef

Hassold T, Hunt P. To err (meiotically) is human: the genesis of human aneuploidy. Nat Rev Genet. 2001;2(4):280–91. doi:10.1038/35066065.PubMedCrossRef

Humphrey MM, Apte SM. The use of minimally invasive surgery for endometrial cancer. Cancer Control : J Moffitt Cancer Center. 2009;16(1):30–7.

Krey LC, Grifo JA. Poor embryo quality: the answer lies (mostly) in the egg. Fertil Steril. 2001;75(3):466–8.PubMedCrossRef

Lim AS, Tsakok MF. Age-related decline in fertility: a link to degenerative oocytes? Fertil Steril. 1997;68(2):265–71.PubMedCrossRef

Lister LM, Kouznetsova A, Hyslop LA, Kalleas D, Pace SL, Barel JC, et al. Age-related meiotic segregation errors in mammalian oocytes are preceded by depletion of cohesin and Sgo2. Curr Biol: CB. 2010;20(17):1511–21. doi:10.1016/j.cub.2010.08.023.PubMedCrossRef

McLaren A. Free-range eggs? Science (New York, NY). 2007;316(5823):339. doi:10.1126/science.1142267.CrossRef

10.

Morita J, Terada Y, Hosoi Y, Fujinami N, Sugimoto M, Nakamura S-I, et al. Microtubule organization during rabbit fertilization by intracytoplasmic sperm injection with and without sperm centrosome. Reprod Med Biol. 2005;4(2):169–78. doi:10.1111/j.1447-0578.2005.00096.x.CrossRef

11.

Revel A, Safran A, Benshushan A, Shushan A, Laufer N, Simon A. In vitro maturation and fertilization of oocytes from an intact ovary of a surgically treated patient with endometrial carcinoma: case report. Hum Reprod (Oxford, England). 2004;19(7):1608–11. doi:10.1093/humrep/deh241.CrossRef

12.

Selesniemi K, Lee HJ, Muhlhauser A, Tilly JL. Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies. Proc Natl Acad Sci U S A. 2011;108(30):12319–24. doi:10.1073/pnas.1018793108.PubMedCrossRef

13.

Subramanian VV, Bickel SE. Aging predisposes oocytes to meiotic nondisjunction when the cohesin subunit SMC1 is reduced. PLoS Genet. 2008;4(11):e1000263. doi:10.1371/journal.pgen.1000263.PubMedCrossRef

14.

Vogt E, Kirsch-Volders M, Parry J, Eichenlaub-Ritter U. Spindle formation, chromosome segregation and the spindle checkpoint in mammalian oocytes and susceptibility to meiotic error. Mutat Res. 2008;651(1–2):14–29. doi:10.1016/j.mrgentox.2007.10.015.PubMed

15.

Wiser A, Son WY, Shalom-Paz E, Reinblatt SL, Tulandi T, Holzer H. How old is too old for in vitro maturation (IVM) treatment? Eur J Obstet Gynecol Reprod Biol. 2011;159(2):381–3. doi:10.1016/j.ejogrb.2011.09.009.PubMedCrossRef

Title: Retrieval and in vitro maturation of human oocytes from ovaries removed during surgery for endometrial carcinoma: a novel strategy for human oocyte research
Authors: Hiromitsu Shirasawa
Jin Kumagai
Wataru Sato
Yukiyo Kumazawa
Naoki Sato
Yukihiro Terada
Publication date: 01-09-2013
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 9/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-0040-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Retrieval and in vitro maturation of human oocytes from ovaries removed during surgery for endometrial carcinoma: a novel strategy for human oocyte research

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 9/2013

One abstinence day decreases sperm DNA fragmentation in 90 % of selected patients

Depression in Chinese men undergoing different assisted reproductive technique treatments: prevalence and risk factors

Is there a role for DHEA supplementation in women with diminished ovarian reserve?

Association of progesterone receptor gene polymorphism with male infertility and clinical outcome of ICSI

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient