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Journal of Assisted Reproduction and Genetics

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01-09-2013 | Technological Innovations

A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

Authors: Bayasula, Akira Iwase, Hiroharu Kobayashi, Maki Goto, Tatsuo Nakahara, Tomoko Nakamura, Mika Kondo, Yoshinari Nagatomo, Tomomi Kotani, Fumitaka Kikkawa

Published in: Journal of Assisted Reproduction and Genetics | Issue 9/2013

Abstract

Purpose

Human follicular fluid constitutes the microenvironment of follicles and includes various biological active proteins that can affect follicle growth and oocyte fertilization. Conducting proteomic evaluations of human follicular fluid may be helpful for identifying potential biomarkers possibly possessing a predictive value for oocyte quality and the success of in vitro fertilization.

Method

We performed proteomic profiling of human follicular fluids containing oocytes that were fertilized and resulted in pregnancy and follicular fluids containing oocytes that were not fertilized in the same patients undergoing intracytoplasmic sperm injection using the LTQ Orbitrap coupled with liquid chromatography-tandem mass spectrometry (LC/MS/MS) analyses.

Results

We identified a total of 503 proteins in human follicular fluids containing fertilized and non-fertilized oocytes obtained from 12 patients. We also found that 53 proteins exhibited significantly different spectral counts between the two groups, including heparan sulfate proteoglycan perlecan, which showed significant upregulation in the follicular fluids containing fertilized oocytes in comparison with that observed in the follicular fluids containing non-fertilized oocytes.

Conclusion

Our results suggest a possibility that proteins identified by LC/MS/MS in follicular fluid might not only be involved in folliculogenesis, but also function as biomarkers possessing predictive potential for oocyte maturation and the success of IVF when their expression levels are significantly different between fertilized and non-fertilized oocytes, although no distinctive biomarkers were identified in the current study.

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Title: A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient
Authors: Bayasula
Akira Iwase
Hiroharu Kobayashi
Maki Goto
Tatsuo Nakahara
Tomoko Nakamura
Mika Kondo
Yoshinari Nagatomo
Tomomi Kotani
Fumitaka Kikkawa
Publication date: 01-09-2013
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 9/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-0004-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

Abstract

Purpose

Method

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Method

Results

Conclusion

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