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Journal of Assisted Reproduction and Genetics

Published in:

01-06-2011 | Gamete Biology

Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system

Authors: Leila Sadat Tahaei, Hussein Eimani, Poopak Eftekhari Yazdi, Bita Ebrahimi, Rouhollah Fathi

Published in: Journal of Assisted Reproduction and Genetics | Issue 6/2011

Abstract

Purpose

Evaluation of the all-trans retinoic acid (t-RA) effects on in vitro maturation (IVM) and in vitro fertilization (IVF) of immature mouse oocytes in the presence and absence of granulosa cell monolayer.

Methods

Denuded oocytes isolated from mice ovaries and matured in IVM medium alone (Control I), IVM medium in the presence of granulosa cells (Control II), IVM medium with t-RA (Experimental I) and IVM medium simultaneously with t-RA and granulosa cells (Experimental II). After 24 h, matured oocytes were fertilized in T6 medium and their development was followed until the blastocyst stage. Metaphase II oocytes ploidy were evaluated by chromosome counting.

Results

The t-RA group compared to the control groups showed no obvious abnormalities. Additionally maturation and embryo development rates significantly increased in the t-RA treated granulosa cell co-culture system.

Conclusions

In conclusion, association of t-RA with granulosa cell co-culture during in vitro maturation increases meiosis resumption, formation of metaphase II oocytes, as well as 2-cell and blastocyst stage embryos.

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Title: Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system
Authors: Leila Sadat Tahaei
Hussein Eimani
Poopak Eftekhari Yazdi
Bita Ebrahimi
Rouhollah Fathi
Publication date: 01-06-2011
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 6/2011
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9579-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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