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Inflammation
Published in: Inflammation 5/2020

01-10-2020 | Original Article

Transcriptome Landscape of Intracellular Brucella ovis Surviving in RAW264.7 Macrophage Immune System

Authors: Hanwei Jiao, Bowen Li, Zonglin Zheng, Zhixiong Zhou, Wenjie Li, Guojing Gu, Juan Liu, Yichen Luo, Xuehong Shuai, Yu Zhao, Yuxuan Liu, Yidan Wang, Xinglong Wang, Xiaoyan Hu, Li Wu, Jixuan Chen, Qingzhou Huang

Published in: Inflammation | Issue 5/2020

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Abstract

Brucella ovis infection results in genital damage and epididymitis in rams, placental inflammation and rare abortion in ewes, and neonatal mortality in lambs. However, the mechanism underlying B. ovis infection remains unclear. In the present study, we used prokaryotic transcriptome sequencing to identify the differentially expressed genes (DEGs) between wild-type B. ovis and intracellular B. ovis in RAW264.7 macrophages. Gene ontology (GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed, and quantitative reverse transcriptase PCR (qRT-PCR) was used to validate the top 10 upregulated and downregulated DEGs. The results showed that 212 genes were differentially expressed, including 68 upregulated and 144 downregulated genes, which were mainly enriched in 30 GO terms linked to biological process, cellular component, and molecular function. KEGG analysis showed that the DEGs were enriched in the hypoxia-inducible factor 1 (HIF-1) signaling pathway, mitogen-activated protein kinase (MAPK) signaling pathway, beta-alanine metabolism, and quorum sensing pathway. BME_RS01160, BME_RS04270, BME_RS08185, BME_RS12880, BME_RS25875, predicted_RNA865, and predicted_RNA953 were confirmed with the transcriptome sequencing data. Hence, our findings not only reveal the intracellular parasitism of B. ovis in the macrophage immune system, but also help to understand the mechanism of chronic B. ovis infection.
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Metadata
Title
Transcriptome Landscape of Intracellular Brucella ovis Surviving in RAW264.7 Macrophage Immune System
Authors
Hanwei Jiao
Bowen Li
Zonglin Zheng
Zhixiong Zhou
Wenjie Li
Guojing Gu
Juan Liu
Yichen Luo
Xuehong Shuai
Yu Zhao
Yuxuan Liu
Yidan Wang
Xinglong Wang
Xiaoyan Hu
Li Wu
Jixuan Chen
Qingzhou Huang
Publication date
01-10-2020
Publisher
Springer US
Published in
Inflammation / Issue 5/2020
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-020-01239-4

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