Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Inflammation
Published in: Inflammation 2/2012

01-04-2012

Taurine Attenuates Liver Injury by Downregulating Phosphorylated p38 MAPK of Kupffer Cells in Rats with Severe Acute Pancreatitis

Authors: Sidong Wei, Qingyong Huang, Jinzheng Li, Zuojin Liu, Haibo You, Yong Chen, Jianping Gong

Published in: Inflammation | Issue 2/2012

Login to get access

Abstract

This study was undertaken to clarify the effects of taurine on liver injury in rats with severe acute pancreatitis (SAP). Rats were randomly assigned to three groups: a sham operation (SO), a SAP (established by infusion of 5% taurocholate), and a SAP given taurine (Taur). At 12 and 24 h post-operation, taurine pretreatment significantly attenuated hepatic tissue injury induced by SAP, and concurrently, serum alanine aminotransferase, aspartate transaminase, and amylase levels were significantly reduced by taurine pretreatment. Compared with the SO group, the total and phosphorylated p38 mitogen-activated protein kinase (p38 MAPK) expression and nuclear factor-κB (NF-κB) activity of Kupffer cells (KCs) were significantly higher in the SAP group, but taurine pretreatment inhibited the total and phosphorylated p38 MAPK expression and NF-κB activity of KCs in the SAP group. The increase of tumor necrosis factor-α and interleukin-lβ in cultured supernate of the SAP rat-derived KCs was also significantly inhibited by taurine pretreatment. These results suggest that taurine pretreatment ameliorated liver injury in rats with SAP mainly by inhibiting phosphorylated p38 MAPK and NF-κB activity in KCs, which may play an important role in liver injury.
Literature
1.
Petrov, M.S., S. Shanbhag, M. Chakraborty, A.R. Phillips, and J.A. Windsor. 2010. Organ failure and infection of pancreatic necrosis as determinants of mortality in patients with acute pancreatitis. Gastroenterology 139: 813–820.PubMedCrossRef
2.
Folch-Puy, E. 2007. Importance of the liver in systemic complications associated with acute pancreatitis: The role of Kupffer cells. The Journal of Pathology 211: 383–388.PubMedCrossRef
3.
Ren, H.B., Z.S. Li, G.M. Xu, Z.X. Tu, X.G. Shi, Y.T. Jia, and Y.F. Gong. 2004. Dynamic changes of mitogen-activated protein kinase signal transduction in rats with severe acute pancreatitis. Chinese Journal of Digestive Diseases 5: 123–125.PubMedCrossRef
4.
Chen, S.R., X.Z. Xu, Y.H. Wang, J.W. Chen, S.W. Xu, L.Q. Gu, and P.Q. Liu. 2010. Icariin derivative inhibits inflammation through suppression of p38 mitogen-activated protein kinase and nuclear factor-kappaB pathways. Biological & Pharmaceutical Bulletin 33: 1307–1313.CrossRef
5.
Yang, M., J. Wu, C.M. Martin, P.R. Kvietys, and T. Rui. 2008. Important role of p38 MAP kinase/NF-kappaB signaling pathway in the sepsis-induced conversion of cardiac myocytes to a proinflammatory phenotype. American Journal of Physiology. Heart and Circulatory Physiology 294: H994–H1001.PubMedCrossRef
6.
Rakonczay, Z.J., P. Hegyi, T. Takács, J. Mccarroll, and A.K. Saluja. 2008. The role of NF-kappaB activation in the pathogenesis of acute pancreatitis. Gut 57: 259–267.PubMedCrossRef
7.
Schindler, J.F., J.B. Monahan, and W.G. Smith. 2007. P38 pathway kinases as anti-inflammatory drug targets. Journal of Dental Research 86: 800–811.PubMedCrossRef
8.
Chen, P., Y. Zhang, M. Qiao, and Y. Yuan. 2007. Activated protein C, an anticoagulant polypeptide, ameliorates severe acute pancreatitis via regulation of mitogen-activated protein kinases. Journal of Gastroenterology 42: 887–896.PubMedCrossRef
9.
Yubero, S., L. Ramudo, M.A. Manso, and I. De Dios. 2009. Mechanisms of dexamethasone-mediated chemokine down-regulation in mild and severe acute pancreatitis. Biochimica et Biophysica Acta 1792: 1205–1211.PubMed
10.
Kylänpää, M.L., H. Repo, and P.A. Puolakkainen. 2010. Inflammation and immunosuppression in severe acute pancreatitis. World Journal of Gastroenterology 16: 2867–2872.PubMedCrossRef
11.
Ei Idrissi, A. 2008. Taurine improves learning and retention in aged mice. Neuroscience Letters 436: 19–22.CrossRef
12.
Wójcik, O.P., K.L. Koenig, A. Zeleniuch-Jacquotte, M. Costa, and Y. Chen. 2010. The potential protective effects of taurine on coronary heart disease. Atherosclerosis 208: 19–25.PubMedCrossRef
13.
Fallahzadeh, M.K., M.R. Namazi, and R.C. Gupta. 2010. Taurine: A potential novel addition to the anti-systemic sclerosis weaponry. Archives of Medical Research 41: 59–61.PubMedCrossRef
14.
Ozturk, M., M.R. Mas, M. Yasar, C. Akay, H. Aydogan, S. Deveci, B. Comert, I. Simsek, N. Mas, and I.H. Kocar. 2003. The role of inducible nitric oxide synthase inhibitor, meropenem, and taurine in experimental acute necrotizing pancreatitis. Pancreas 26: 357–362.PubMedCrossRef
15.
Ahn, B.O., K.H. Kim, G. Lee, H.S. Lee, C.D. Kim, Y.S. Kim, M.W. Son, W.B. Kim, T.Y. Oh, and J.H. Hyun. 2001. Effects of taurine on cerulein-induced acute pancreatitis in the rat. Pharmacology 63: 1–7.PubMedCrossRef
16.
Zhang, F., Y. Mao, H. Qiao, H. Jiang, H. Zhao, X. Chen, L. Tong, and X. Sun. 2010. Protective effects of taurine against endotoxin-induced acute liver injury after hepatic ischemia reperfusion. Amino Acids 38: 237–245.PubMedCrossRef
17.
Laskin, D.L., L. Chen, P.A. Hankey, and J.D. Laskin. 2010. Role of STK in mouse liver macrophage and endothelial cell responsiveness during acute endotoxemia. Journal of Leukocyte Biology 88: 373–382.PubMedCrossRef
18.
Wittel, U.A., T. Wiech, S. Chakraborty, B. Boss, R. Lauch, S.K. Batra, and U.T. Hopt. 2008. Taurocholate-induced pancreatitis: A model of severe necrotizing pancreatitis in mice. Pancreas 36: e9–e21.PubMedCrossRef
19.
Bollen, T.L., H.C. van Santvoort, M.G. Besselink, M.S. van Leeuwen, K.D. Horvath, P.C. Freeny, and H.G. Gooszen. 2008. The Atlanta Classification of acute pancreatitis revisited. British Journal of Surgery 95: 6–21.PubMedCrossRef
20.
Liu, Z.J., L.N. Yan, X.H. Li, F.L. Xu, X.F. Chen, H.B. You, and J.P. Gong. 2008. Up-regulation of IRAK-M is essential for endotoxin tolerance induced by a low dose of lipopolysaccharide in Kupffer cells. Journal of Surgical Research 150: 34–39.PubMedCrossRef
21.
Liao, R., Z. Liu, S. Wei, F. Xu, Z. Chen, and J. Gong. 2009. Triggering receptor in myeloid cells (TREM-1) specific expression in peripheral blood mononuclear cells of sepsis patients with acute cholangitis. Inflammation 32: 182–190.PubMedCrossRef
22.
Singh, M., A.K. Chaudhary, S. Pandya, S. Debnath, M. Singh, P.A. Singh, and R. Mehrotra. 2010. Morphometric analysis in potentially malignant head and neck lesions: Oral submucous fibrosis. Asian Pacific Journal of Cancer Prevention 11: 257–260.PubMed
23.
Chen, Y., T. Yan, L.J. Shi, Z. Liu, S.Y. Liang, X.F. Luan, F.W. Long, J. Chen, Y. Peng, L.N. Yan, and J.P. Gong. 2010. Knockdown of interleukin-2 by shRNA-mediated RNA interference prolongs liver allograft survival. Journal of Surgical Research 159: 582–587.PubMedCrossRef
24.
Schmidt, J., D.W. Rattner, K. Lewandrowski, C.C. Compton, U. Mandavilli, W.T. Knoefel, and A.L. Warshaw. 1992. A better model of acute pancreatitis for evaluating therapy. Annals of Surgery 215: 44–56.PubMedCrossRef
25.
Karen, M., O. Yuksel, N. Akyürek, E. Ofluoğlu, K. Cağlar, T.T. Sahin, H. Paşaoğlu, L. Memiş, N. Akyürek, and H. Bostanci. 2010. Probiotic agent Saccharomyces boulardii reduces the incidence of lung injury in acute necrotizing pancreatitis induced rats. Journal of Surgical Research 160: 139–144.PubMedCrossRef
26.
Pang, H.Y., G. Liu, and G.T. Liu. 2009. Compound FLZ inhibits lipopolysaccharide-induced inflammatory effects via down-regulation of the TAK-IKK and TAK-JNK/p38MAPK pathways in RAW264.7 macrophages. Acta Pharmacologica Sinica 30: 209–218.PubMedCrossRef
27.
Chen, Y., Z. Liu, S. Liang, X. Luan, F. Long, J. Chen, Y. Peng, L. Yan, and J. Gong. 2008. Role of Kupffer cells in the induction of tolerance of orthotopic liver transplantation in rats. Liver Transplantation 14: 823–836.PubMedCrossRef
28.
Schneider, L., T. Hackert, T. Longerich, W. Hartwig, S. Fritz, R. Krych, F. Fortunato, M.M. Gebhard, and J. Werner. 2010. Effects of gadolinium chloride and glycine on hepatic and pancreatic tissue damage in alcoholic pancreatitis. Pancreas 39: 502–509.PubMedCrossRef
29.
Das, J., J. Ghosh, P. Manna, and P.C. Sil. 2010. Acetaminophen induced acute liver failure via oxidative stress and JNK activation: Protective role of taurine by the suppression of cytochrome P4502E1. Free Radical Research 44: 340–355.PubMedCrossRef
30.
Twait, E., D.E. Williard, and I. Samuel. 2010. Dominant negative p38 mitogen-activated protein kinase expression inhibits NF-kappaB activation in AR42J cells. Pancreatology 10: 119–128.PubMedCrossRef
31.
Yang, J., A. Fier, Y. Carter, G. Liu, P.K. Epling-Burnette, F. Bai, T.P. Loughran, S. Mastorides, J.G. Norman, and M.M. Murr. 2003. Liver injury during acute pancreatitis: The role of pancreatitis-associated ascitic fluid (PAAF), p38-MAPK, and caspase-3 in inducing hepatocyte apoptosis. Journal of Gastrointestinal Surgery 7: 200–207.PubMedCrossRef
32.
Ramudo, L., S. Yubero, M.A. Manso, S. Vicente, and I. De Dios. 2009. Signal transduction of MCP-1 expression induced by pancreatitis-associated ascitic fluid in pancreatic acinar cells. Journal of Cellular and Molecular Medicine 13: 1314–1320.PubMedCrossRef
33.
Liu, H.S., C.E. Pan, Q.G. Liu, W. Yang, and X.M. Liu. 2003. Effect of NF-kappaB and p38 MAPK in activated monocytes/macrophages on pro-inflammatory cytokines of rats with acute pancreatitis. World Journal of Gastroenterology 9: 2513–2518.PubMed
34.
Xu, F.L., H.B. You, X.H. Li, X.F. Chen, Z.J. Liu, and J.P. Gong. 2008. Glycine attenuates endotoxin-induced liver injury through down-regulation of TLR4 signaling in Kupffer cell. The American Journal of Surgery 196: 139–148.CrossRef
35.
Gallagher, S.F., J. Yang, K. Baksh, K. Haines, H. Carpenter, P.K. Epling-Burnette, Y. Peng, J. Norman, and M.M. Murr. 2004. Acute pancreatitis induces FasL gene expression and apoptosis in the liver. Journal of Surgical Research 122: 201–209.PubMedCrossRef
36.
Yuan, J., A. Lugea, L. Zheng, I. Gukovsky, M. Edderkaoui, E. Rozengurt, and S.J. Pandol. 2008. Protein kinase D1 mediates NF-kappaB activation induced by cholecystokinin and cholinergic signaling in pancreatic acinar cells. American Journal of Physiology. Gastrointestinal and Liver Physiology 295: G1190–G1201.PubMedCrossRef
37.
Roy, A., P. Manna, and P.C. Sil. 2009. Prophylactic role of taurine on arsenic mediated oxidative renal dysfunction via MAPKs/NF-kappaB and mitochondria dependent pathways. Free Radical Research 43: 995–1007.PubMedCrossRef
38.
Kim, C., Y. Sano, K. Todorova, B.A. Carlson, L. Arpa, A. Celada, T. Lawrence, K. Otsu, J.L. Brissette, J.S. Arthur, and J.M. Park. 2008. The kinase p38 alpha serves cell type-specific inflammatory functions in skin injury and coordinates pro- and anti-inflammatory gene expression. Nature Immunology 9: 1019–1027.PubMedCrossRef
39.
Gloor, B., T.A. Blinman, D.A. Rigberg, K.E. Todd, J.S. Lane, O.J. Hines, and H.A. Reber. 2000. Kupffer cell blockade reduces hepatic and systemic cytokine levels and lung injury in hemorrhagic pancreatitis in rats. Pancreas 21: 414–420.PubMedCrossRef
40.
Liu, H.B., N.Q. Cui, D.H. Li, and C. Chen. 2006. Role of Kupffer cells in acute hemorrhagic necrotizing pancreatitis-associated lung injury of rats. World Journal of Gastroenterology 12: 403–407.PubMed
41.
Choi, O.S., S.J. Park, S.W. Seo, C.S. Park, J.J. Cho, and H.J. Ahn. 2005. The 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, lovastatin (statin) ameliorates CCK-induced acute pancreatitis in rats. Biological & Pharmaceutical Bulletin 28: 1394–1397.CrossRef
42.
Sturman, J.A., G.W. Hepner, A.F. Hofmann, and P.J. Thomas. 1975. Metabolism of [35S] taurine in man. Journal of Nutrition 105: 1206–1214.PubMed
43.
Minor, T., T. Yamaguchi, H. Klauke, P. Wingenfeld, D. Michalk, and W. Isselhard. 1996. Taurine reduces experimental liver injury after cold ischemic preservation and a period of rewarming prior to reperfusion. Advances in Experimental Medicine and Biology 403: 157–161.PubMed
44.
Murr, M.M., J. Yang, A. Fier, S.F. Gallagher, G. Carter, W.R. Gower Jr., and J.G. Norman. 2003. Regulation of Kupffer cell TNF gene expression during experimental acute pancreatitis: The role of p38-MAPK, ERK1/2, SAPK/JNK, and NF-kappaB. Journal of Gastrointestinal Surgery 7: 20–25.PubMedCrossRef
Metadata
Title
Taurine Attenuates Liver Injury by Downregulating Phosphorylated p38 MAPK of Kupffer Cells in Rats with Severe Acute Pancreatitis
Authors
Sidong Wei
Qingyong Huang
Jinzheng Li
Zuojin Liu
Haibo You
Yong Chen
Jianping Gong
Publication date
01-04-2012
Publisher
Springer US
Published in
Inflammation / Issue 2/2012
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-011-9362-0

Other articles of this Issue 2/2012

Inflammation 2/2012 Go to the issue

OriginalPaper

Moringa oleifera Pod Inhibits Inflammatory Mediator Production by Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage Cell Lines

OriginalPaper

Ethanol Extract of Elaeocarpus petiolatus Inhibits Lipopolysaccharide-Induced Inflammation in Macrophage Cells

OriginalPaper

Farnesyltransferase Inhibitor Manumycin Targets IL1β-Ras-HIF-1α Axis in Tumor Cells of Diverse Origin

OriginalPaper

Vasodilator Phosphostimulated Protein (VASP) Protects Endothelial Barrier Function During Hypoxia

OriginalPaper

Modulation of Inflammatory Processes by Leaves Extract from Clusia nemorosa Both In Vitro and In Vivo Animal Models

OriginalPaper

Alpinia katsumadai HAYATA Seed Extract Inhibit LPS-Induced Inflammation by Induction of Heme Oxygenase-1 in RAW264.7 Cells
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine
Image Credits