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Digestive Diseases and Sciences
Published in: Digestive Diseases and Sciences 1/2017

01-01-2017 | Original Article

Nitric Oxide and Hydrogen Sulfide Interact When Modulating Gastric Physiological Functions in Rodents

Authors: Larisse Tavares Lucetti, Renan Oliveira Silva, Ana Paula Macedo Santana, Bruno de Melo Tavares, Mariana Lima Vale, Pedro Marcos Gomes Soares, Francisco José Batista de Lima Júnior, Pedro Jorge Caldas Magalhães, Fernando de Queiroz Cunha, Ronaldo de Albuquerque Ribeiro, Jand-Venes Rolim Medeiros, Marcellus Henrique Loiola Ponte Souza

Published in: Digestive Diseases and Sciences | Issue 1/2017

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Abstract

Aim

The objective was to evaluate the effects of nitric oxide (NO) and hydrogen sulfide (H2S) donors and possible interactions between these two systems in modulating gastric function.

Methods

Mice received saline, sodium nitroprusside (SNP), or sodium hydrosulfite (NaHS), and after 1 h, the animals were killed for immunofluorescence analysis of CSE or eNOS expressions, respectively. Other groups received saline, SNP, NaHS, Lawesson’s reagent (H2S donor), PAG + SNP, l-NAME, l-NAME + NaHS, or l-NAME + Lawesson’s reagent. Then, the gastric secretions (mucous and acid), gastric blood flow, gastric defense against ethanol, and gastric motility (gastric emptying and gastric contractility) were evaluated.

Results

SNP and NaHS increased the expression of CSE or eNOS, respectively. SNP or Lawesson’s reagent did not alter gastric acid secretion but increased mucus production, and these effects reverted with PAG and l-NAME treatment, respectively. SNP or NaHS increased gastric blood flow and protected the gastric mucosa against ethanol injury, and these effects reverted with PAG and l-NAME treatments, respectively. SNP delayed gastric emptying when compared with saline, and PAG partially reversed this effect. NaHS accelerate gastric emptying, and l-NAME partially reversed this effect. SNP and NaHS alone induced gastric fundus and pylorus relaxation. However, pretreatment with PAG or l-NAME reversed these relaxant effects only in the pylorus but not in the gastric fundus.

Conclusion

NO and H2S interact in gastric physiological functions, and this “cross-talk” is important in the control of mucus secretion, gastric blood flow, gastric mucosal defense, and gastric motility, but not in the control of basal gastric acid secretion.
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Metadata
Title
Nitric Oxide and Hydrogen Sulfide Interact When Modulating Gastric Physiological Functions in Rodents
Authors
Larisse Tavares Lucetti
Renan Oliveira Silva
Ana Paula Macedo Santana
Bruno de Melo Tavares
Mariana Lima Vale
Pedro Marcos Gomes Soares
Francisco José Batista de Lima Júnior
Pedro Jorge Caldas Magalhães
Fernando de Queiroz Cunha
Ronaldo de Albuquerque Ribeiro
Jand-Venes Rolim Medeiros
Marcellus Henrique Loiola Ponte Souza
Publication date
01-01-2017
Publisher
Springer US
Published in
Digestive Diseases and Sciences / Issue 1/2017
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI
https://doi.org/10.1007/s10620-016-4377-x

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