Transforming Growth Factor and Nuclear Factor Kappa B Mediated Prophylactic Cardioprotection by Total Flavonoids of Fructus Chorspondiatis in Myocardial Ischemia

In this study, we extracted Total Flavonoids of Fructus Chorspondiatis (TFFC), a potential effective component from Chorspomias axillaries Batt et Hill to investigate its effect on myocardial ischemia induced by isoproteronol in rat and elucidated its probable mechanism.

The results showed that prophylactically ``intragastrical administration of TFFC at the dose of 200 mg/kg body weight effectively suppressed the variation of J points in electrocardiogram (0.211 ± 0.059 mV versus 0.277 ± 0.046 mV, p < 0.05) and inhibited the upregulated serum level of creatine kinase (754 ± 114 U/l versus 1,112 ± 239 U/l, p < 0.05), creatine kinase -MB (700 ± 95 U/l versus 1,012 ± 252 U/l, p < 0.05) and lactate dehydrogenase (1,174 ± 200 U/l versus 2,025 ± 975 U/l, p < 0.05) in myocardial ischemia, revealing its cardioprotective effect. It increased a potent tissue protective polypeptide, transforming growth factor β₁ (TGF-β₁) level as well as its receptors TβRI and TβRII(p < 0.05) and significantly inhibited the expression of a redox-sensitive transcription factor, nuclear factor kappa B (NF-κB).

These data demonstrated that TFFC at the dose of 200 mg/kg body weight exerts prophylactically cardioprotective effect during ischemia injury. Part of its cardioprotective mechanism may relate to induction of TGF-β₁ to competitively inhibit NF-κB signaling pathway. Prophylactically exogenous administration with the component may serve as a novel therapeutic strategy for ischemic cardiovascular diseases.