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01-11-2012 | Preclinical Study

Deep sequencing reveals small RNA characterization of invasive micropapillary carcinomas of the breast

Authors: Shuai Li, Cuicui Yang, Lili Zhai, Wenwei Zhang, Jing Yu, Feng Gu, Ronggang Lang, Yu Fan, Meihua Gong, Xiuqing Zhang, Li Fu

Published in: Breast Cancer Research and Treatment | Issue 1/2012

Abstract

Invasive micropapillary carcinoma (IMPC) is an uncommon histological type of breast cancer. IMPC has a special growth pattern and a more aggressive behavior than invasive ductal carcinomas of no special types (IDC-NSTs). microRNAs are a large class of non-coding RNAs involved in the regulation of various biological processes. Here, we analyzed the small RNA transcriptomes of five formalin-fixed paraffin-embedded (FFPE) pure IMPC samples and five FFPE IDC-NSTs samples by means of next-generation sequencing, generating a total of >170,000,000 clean reads. In an unsupervised cluster analysis, differently expressed miRNAs generated a tree with clear distinction between IMPC and IDC-NSTs classes. Paired fresh-frozen and FFPE specimens showed very similar miRNA expression profiles. By means of RT-qPCR, we further investigated miRNA expression in more IMPC (n = 22) and IDC-NSTs (n = 24) FFPE samples and found let-7b, miR-30c, miR-148a, miR-181a, miR-181a*, and miR-181b were significantly differently expressed between the two groups. We also elucidated several features of miRNA in these breast cancer tissues including 5′ variability, miRNA editing, and 3′ untemplated addition. Our findings will lead to further understanding of the invasive potency of IMPC and gain an insight into the diversity and complexity of small RNA molecules in breast cancer tissues.

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Title: Deep sequencing reveals small RNA characterization of invasive micropapillary carcinomas of the breast
Authors: Shuai Li
Cuicui Yang
Lili Zhai
Wenwei Zhang
Jing Yu
Feng Gu
Ronggang Lang
Yu Fan
Meihua Gong
Xiuqing Zhang
Li Fu
Publication date: 01-11-2012
Publisher: Springer US
Published in: Breast Cancer Research and Treatment / Issue 1/2012
Print ISSN: 0167-6806
Electronic ISSN: 1573-7217
DOI: https://doi.org/10.1007/s10549-012-2166-6

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