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Open Access 01-05-2015 | Original Article

Cross-linked xenogenic collagen implantation in the sheep model for vaginal surgery

Authors: Masayuki Endo, Iva Urbankova, Jaromir Vlacil, Siddarth Sengupta, Thomas Deprest, Bernd Klosterhalfen, Andrew Feola, Jan Deprest

Published in: Gynecological Surgery | Issue 2/2015

Abstract

The properties of meshes used in reconstructive surgery affect the host response and biomechanical characteristics of the grafted tissue. Whereas durable synthetics induce a chronic inflammation, biological grafts are usually considered as more biocompatible. The location of implantation is another determinant of the host response: the vagina is a different environment with specific function and anatomy. Herein, we evaluated a cross-linked acellular collagen matrix (ACM), pretreated by the anti-calcification procedure ADAPT® in a sheep model for vaginal surgery. Ten sheep were implanted with a cross-linked ACM, and six controls were implanted with a polypropylene (PP; 56 g/m²) control. One implant was inserted in the lower rectovaginal septum, and one was used for abdominal wall defect reconstruction. Grafts were removed after 180 days; all graft-related complications were recorded, and explants underwent bi-axial tensiometry and contractility testing. Half of ACM-implanted animals had palpable induration in the vaginal implantation area, two of these also on the abdominal implant. One animal had a vaginal exposure. Vaginal ACMs were 63 % less stiff compared to abdominal ACM explants (p = 0.01) but comparable to vaginal PP explants. Seven anterior vaginal ACM explants showed areas of graft degradation on histology. There was no overall difference in vaginal contractility. Considering histologic degradation in the anterior vaginal implant as representative for the host, posterior ACM explants of animals with degradation had a 60 % reduced contractility as compared to PP (p = 0.048). Three abdominal implants showed histologic degradation; those were more compliant than non-degraded implants. Vaginal implantation with ACM was associated with graft-related complications (GRCs) and biomechanical properties comparable to PP. Partially degraded ACM had a decreased vaginal contractility.

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Title: Cross-linked xenogenic collagen implantation in the sheep model for vaginal surgery
Authors: Masayuki Endo
Iva Urbankova
Jaromir Vlacil
Siddarth Sengupta
Thomas Deprest
Bernd Klosterhalfen
Andrew Feola
Jan Deprest
Publication date: 01-05-2015
Publisher: Springer Berlin Heidelberg
Published in: Gynecological Surgery / Issue 2/2015
Print ISSN: 1613-2076
Electronic ISSN: 1613-2084
DOI: https://doi.org/10.1007/s10397-015-0883-7

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Cross-linked xenogenic collagen implantation in the sheep model for vaginal surgery

Abstract

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Abstract

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