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Open Access 01-09-2008 | Original Contribution

Sphincter Contractility After Muscle-Derived Stem Cells Autograft into the Cryoinjured Anal Sphincters of Rats

Authors: Sung-Bum Kang, M.D., Ph.D., Haet Nim Lee, M.A., Ji Young Lee, M.A., Jun-Seok Park, M.D., Hye Seung Lee, M.D., Ph.D., Ji Youl Lee, M.D., Ph.D.

Published in: Diseases of the Colon & Rectum | Issue 9/2008

Abstract

Purpose

This study was designed to determine whether the injection of muscle-derived stem cells into the anal sphincter can improve functional properties in a fecal incontinence rat model.

Methods

Cryoinjured rats were utilized as a fecal incontinence model. The gastrocnemius muscles of normal three-week-old female Sprague-Dawley rats were used for the purification of the muscle-derived stem cells. The experimental group was divided into three subgroups: normal control; cryoinjured; and muscle-derived stem cells (3 × 10⁶ cells) injection group of cryoinjured rats. All groups were subsequently employed in contractility experiments using muscle strips from the anal sphincter, one week after preparation.

Results

Contractility in the cryoinjured group was significantly lower than in the control after treatment with acetylcholine and KCl. In the muscle-derived stem cells injection group, contraction amplitude was higher than in the cryoinjured group but not significantly (20.5 ± 21.3 vs. 17.3 ± 3.4 g per gram tissue, with acetylcholine (10⁻⁴ mol/l); 31 ± 14.2 vs. 18.4 ± 7.9 g per gram tissue, with KCl (10⁻⁴ mol/l)). PKH-26-labeled transplanted cells were detected in all of the grafted sphincters. Differentiated muscle masses stained positively for alpha smooth muscle actin and myosin heavy chain at the muscle-derived stem cells injection sites.

Conclusions

This is the first study reporting that autologous muscle-derived stem cell grafts may be a tool for improving anal sphincter function.

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Title: Sphincter Contractility After Muscle-Derived Stem Cells Autograft into the Cryoinjured Anal Sphincters of Rats
Authors: Sung-Bum Kang, M.D., Ph.D.
Haet Nim Lee, M.A.
Ji Young Lee, M.A.
Jun-Seok Park, M.D.
Hye Seung Lee, M.D., Ph.D.
Ji Youl Lee, M.D., Ph.D.
Publication date: 01-09-2008
Publisher: Springer-Verlag
Published in: Diseases of the Colon & Rectum / Issue 9/2008
Print ISSN: 0012-3706
Electronic ISSN: 1530-0358
DOI: https://doi.org/10.1007/s10350-008-9360-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Sphincter Contractility After Muscle-Derived Stem Cells Autograft into the Cryoinjured Anal Sphincters of Rats

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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