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Magnetic Resonance Materials in Physics, Biology and Medicine

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01-12-2015 | Research Article

Parameterization of hyperpolarized ¹³C-bicarbonate-dissolution dynamic nuclear polarization

Authors: David Johannes Scholz, Angela M. Otto, Josef Hintermair, Franz Schilling, Annette Frank, Ulrich Köllisch, Martin A. Janich, Rolf F. Schulte, Markus Schwaiger, Axel Haase, Marion I. Menzel

Published in: Magnetic Resonance Materials in Physics, Biology and Medicine | Issue 6/2015

Abstract

Objective

¹³C metabolic MRI using hyperpolarized ¹³C-bicarbonate enables preclinical detection of pH. To improve signal-to-noise ratio, experimental procedures were refined, and the influence of pH, buffer capacity, temperature, and field strength were investigated.

Materials and methods

Bicarbonate preparation was investigated. Bicarbonate was prepared and applied in spectroscopy at 1, 3, 14 T using pure dissolution, culture medium, and MCF-7 cell spheroids. Healthy rats were imaged by spectral–spatial spiral acquisition for spatial and temporal bicarbonate distribution, pH mapping, and signal decay analysis.

Results

An optimized preparation technique for maximum solubility of 6 mol/L and polarization levels of 19–21 % is presented; T₁ and SNR dependency on field strength, buffer capacity, and pH was investigated. pH mapping in vivo is demonstrated.

Conclusion

An optimized bicarbonate preparation and experimental procedure provided improved T₁ and SNR values, allowing in vitro and in vivo applications.

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Title: Parameterization of hyperpolarized 13C-bicarbonate-dissolution dynamic nuclear polarization
Authors: David Johannes Scholz
Angela M. Otto
Josef Hintermair
Franz Schilling
Annette Frank
Ulrich Köllisch
Martin A. Janich
Rolf F. Schulte
Markus Schwaiger
Axel Haase
Marion I. Menzel
Publication date: 01-12-2015
Publisher: Springer Berlin Heidelberg
Published in: Magnetic Resonance Materials in Physics, Biology and Medicine / Issue 6/2015
Print ISSN: 0968-5243
Electronic ISSN: 1352-8661
DOI: https://doi.org/10.1007/s10334-015-0500-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Parameterization of hyperpolarized ¹³C-bicarbonate-dissolution dynamic nuclear polarization

Abstract

Objective

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Materials and methods

Results

Conclusion

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