Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Journal of Infection and Chemotherapy
Published in: Journal of Infection and Chemotherapy 3/2013

01-06-2013 | Review Article

Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Authors: Yasuyoshi Mori, Hidetoshi Kanda, Tsugunori Notomi

Published in: Journal of Infection and Chemotherapy | Issue 3/2013

Login to get access

Abstract

Loop-mediated isothermal amplification (LAMP) is an established technology that continues to attract the attention of researchers in many fields. Research and development efforts on LAMP technology in recent years have focused on two major areas; first, the study of its clinical application as an approved in vitro diagnostics tool in Japan and certain other countries; and second, research aimed at further simplifying the LAMP test process. This review provides an overview of the status of LAMP on these two topics by summarizing research work conducted, in the main, after our previous review article.
Literature
1.
Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28:E63.PubMedCrossRef
2.
Mori Y, Notomi T. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother. 2009;15:62–9.PubMedCrossRef
3.
Parida M, Sannarangaiah S, Dash PK, Rao PV, Morita K. Loop-mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev Med Virol. 2008;18:407–21.PubMedCrossRef
4.
Njiru ZK. Loop-mediated isothermal amplification technology: towards point of care diagnostics. PLoS Negl Trop Dis. 2012;6:e1572.PubMedCrossRef
5.
Tamaki Y, Akiyama F, Iwase T, Kaneko T, Tsuda H, Sato K, et al. Molecular detection of lymph node metastases in breast cancer patients: results of a multicenter trial using the one-step nucleic acid amplification assay. Clin Cancer Res. 2009;15:2879–84.PubMedCrossRef
6.
Mitarai S, Okumura M, Toyota E, Yoshiyama T, Aono A, Sejimo A, et al. Evaluation of a simple loop-mediated isothermal amplification test kit for the diagnosis of tuberculosis. Int J Tuberc Lung Dis. 2011;15:1211–7.PubMedCrossRef
7.
Polley SD, Mori Y, Watson J, Perkins MD, González IJ, Notomi T, et al. Mitochondrial DNA targets increase sensitivity of malaria detection using loop-mediated isothermal amplification. J Clin Microbiol. 2010;48:2866–71.PubMedCrossRef
8.
Polley SD, González IJ, Mohamed D, Daly R, Bowers K. et al. Clinical evaluation of a LAMP test kit for diagnosis of imported malaria. J Infect Dis.
9.
Njiru ZK, Mikosza AS, Matovu E, Enyaru JC, Ouma JO, Kibona SN. African trypanosomiasis: sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA. Int J Parasitol. 2008;38:589–99.PubMedCrossRef
10.
11.
Lalande V, Barrault L, Wadel S, Eckert C, Petit JC, Barbut F. Evaluation of a loop-mediated isothermal amplification assay for diagnosis of Clostridium difficile infections. J Clin Microbiol. 2011;49:2714–6.PubMedCrossRef
12.
Han ET, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, et al. Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis. J Clin Microbiol. 2007;45:2521–8.PubMedCrossRef
13.
Sirichaisinthop J, Buates S, Watanabe R, Han ET, Suktawonjaroenpon W, Krasaesub S, et al. Evaluation of loop-mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting. Am J Trop Med Hyg. 2011;85:594–6.PubMedCrossRef
14.
Parida M, Horioke K, Ishida H, Dash PK, Saxena P, Jana AM, et al. Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol. 2005;43:2895–903.PubMedCrossRef
15.
Toriniwa H, Komiya T. Rapid detection and quantification of Japanese encephalitis virus by real-time reverse transcription loop-mediated isothermal amplification. Microbiol Immunol. 2006;50:379–87.PubMed
16.
Parida M, Posadas G, Inoue S, Hasebe F, Morita K. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol. 2004;42:257–63.PubMedCrossRef
17.
Li S, Fang M, Zhou B, Ni H, Shen Q, Zhang H, et al. Simultaneous detection and differentiation of dengue virus serotypes 1-4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay. Virol J 2011;8:360.
18.
Zhang G, Brown EW, González-Escalona N. Comparison of real-time PCR, reverse transcriptase real-time PCR, loop-mediated isothermal amplification, and the FDA conventional microbiological method for the detection of Salmonella spp. in produce. Appl Environ Microbiol 2011;77:6495–6501.
19.
Njiru ZK, Mikosza AS, Armstrong T, Enyaru JC, Ndung’u JM, Thompson AR. Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense. PLoS Negl Trop Dis. 2008;2:e147.PubMedCrossRef
20.
Namangala B, Hachaambwa L, Kajino K, Mweene AS, Hayashida K, Simuunza M, et al. The use of loop-mediated isothermal amplification (LAMP) to detect the re-emerging human African trypanosomiasis (HAT) in the Luangwa and Zambezi Valleys. Parasit Vectors. 2012;5:282.PubMedCrossRef
21.
22.
Lu X, Li X, Mo Z, Jin F, Wang B, Zhao H, et al. Rapid identification of chikungunya and dengue virus by a real-time reverse transcription-loop-mediated isothermal amplification method. Am J Trop Med Hyg. 2012;87:947–53.PubMedCrossRef
23.
Boldbaatar B, Inoue S, Sugiura N, Noguchi A, Orbina JR, Demetria C, et al. Rapid detection of rabies virus by reverse transcription loop-mediated isothermal amplification. Jpn J Infect Dis. 2009;62:187–91.PubMed
24.
Ablordey A, Amissah DA, Aboagye IF, Hatano B, Yamazaki T, Sata T, et al. Detection of Mycobacterium ulcerans by the loop-mediated isothermal amplification method. PLoS Negl Trop Dis. 2012;6:e1590.PubMedCrossRef
25.
de Souza DK, Quaye C, Mosi L, Addo P, Boakye DA. A quick and cost-effective method for the diagnosis of Mycobacterium ulcerans infection. BMC Infect Dis. 2012;12:8.PubMedCrossRef
26.
Mukai T. Development of rapid and simple genomic diagnostic method (in Japanese). Nihon Hansenbyo Gakkai Zasshi 2006;75:265–269
27.
Thekisoe OM, Kuboki N, Nambota A, Fujisaki K, Sugimoto C, Igarashi I, et al. Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomiasis. Acta Trop. 2007;102:182–9.PubMedCrossRef
28.
Thekisoe OM, Rodriguez CV, Rivas F, Coronel-Servian AM, Fukumoto S, Sugimoto C, Kawazu S, Inoue N. Detection of Trypanosoma cruzi and T. rangeli infections from Rhodnius pallescens bugs by loop-mediated isothermal amplification (LAMP). Am J Trop Med Hyg. 2010;82:855–60.PubMedCrossRef
29.
Kuboki N, Inoue N, Sakurai T, Di Cello F, Grab DJ, Suzuki H, Sugimoto C, Igarashi I. Loop-mediated isothermal amplification for detection of African trypanosomes. J Clin Microbiol 2003;41:5517–5524
30.
Takagi H, Itoh M, Islam MZ, Razzaque A, Ekram AR, Hashighuchi Y, et al. Sensitive, specific, and rapid detection of Leishmania donovani DNA by loop-mediated isothermal amplification. Am J Trop Med Hyg. 2009;81:578–82.PubMedCrossRef
31.
Adams ER, Schoone GJ, Ageed AF, Safi SE, Schallig HD. Development of a reverse transcriptase loop-mediated isothermal amplification (LAMP) assay for the sensitive detection of Leishmania parasites in clinical samples. Am J Trop Med Hyg. 2010;82:591–6.PubMedCrossRef
32.
Nkouawa A, Sako Y, Nakao M, Nakaya K, Ito A. Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species. J Clin Microbiol. 2009;47:168–74.PubMedCrossRef
33.
Salant H, Abbasi I, Hamburger J. The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosus coprodetection. Am J Trop Med Hyg. 2012;87:883–7.PubMedCrossRef
34.
Arimatsu Y, Kaewkes S, Laha T, Hong SJ, Sripa B. Rapid detection of Opisthorchis viverrini copro-DNA using loop-mediated isothermal amplification (LAMP). Parasitol Int 2012;61:178–182
35.
Aonuma H, Yoshimura A, Perera N, Shinzawa N, Bando H, Oshiro S, et al. Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model. Parasit Vectors. 2009;2:15.PubMedCrossRef
36.
Wang C, Chen L, Yin X, Hua W, Hou M, Ji M, et al. Application of DNA-based diagnostics in detection of schistosomal DNA in early infection and after drug treatment. Parasit Vectors. 2011;4:164.PubMedCrossRef
37.
Liu CY, Song HQ, Zhang RL, Chen MX, Xu MJ, Ai L, et al. Specific detection of Angiostrongylus cantonensis in the snail Achatina fulica using a loop-mediated isothermal amplification (LAMP) assay. Mol Cell Probes. 2011;25:164–7.PubMedCrossRef
38.
Liang C, Chu Y, Cheng S, Wu H, Kajiyama T, Kambara H, et al. Multiplex loop-mediated isothermal amplification detection by sequence-based barcodes coupled with nicking endonuclease-mediated pyrosequencing. Anal Chem. 2012;84:3758–63.PubMedCrossRef
39.
Solomon AW, Engels D, Bailey RL, Blake IM, Brooker S, Chen JX, et al. A diagnostics platform for the integrated mapping, monitoring, and surveillance of neglected tropical diseases: rationale and target product profiles. PLoS Negl Trop Dis. 2012;6:e1746.PubMedCrossRef
40.
Aonuma H, Suzuki M, Iseki H, Perera N, Nelson B, Igarashi I, et al. Rapid identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification. Biochem Biophys Res Commun. 2008;376:671–6.PubMedCrossRef
41.
Muleya W, Namangala B, Mweene A, Zulu L, Fandamu P, Banda D, et al. Molecular epidemiology and a loop-mediated isothermal amplification method for diagnosis of infection with rabies virus in Zambia. Virus Res. 2012;163:160–8.PubMedCrossRef
42.
Laohasinnarong D, Thekisoe OM, Malele I, Namangala B, Ishii A, Goto Y, et al. Prevalence of Trypanosoma sp. in cattle from Tanzania estimated by conventional PCR and loop-mediated isothermal amplification (LAMP). Parasitol Res. 2011;109:1735–9.PubMedCrossRef
43.
LaBarre P, Hawkins KR, Gerlach J, Wilmoth J, Beddoe A, Singleton J, et al. A simple, inexpensive device for nucleic acid amplification without electricity: toward instrument-free molecular diagnostics in low-resource settings. PLoS ONE. 2011;6:e19738.PubMedCrossRef
44.
Curtis KA, Rudolph DL, Nejad I, Singleton J, Beddoe A, Weigl B, et al. Isothermal amplification using a chemical heating device for point-of-care detection of HIV-1. PLoS ONE. 2012;7:e31432.PubMedCrossRef
45.
Hatano B, Maki T, Obara T, Fukumoto H, Hagisawa K, Matsushita Y, et al. LAMP using a disposable pocket warmer for anthrax detection, a highly mobile and reliable method for anti-bioterrorism. Jpn J Infect Dis. 2010;63:36–40.PubMed
46.
Nkouawa A, Sako Y, Li T, Chen X, Nakao M, Yanagida T. A loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: application to a field survey. Parasitol Int. 2012;61:723–5.PubMedCrossRef
47.
Tanner NA, Zhang Y, Evans TC Jr. Simultaneous multiple target detection in real-time loop-mediated isothermal amplification. Biotechniques. 2012;53:81–9.PubMed
48.
Kaneko H, Kawana T, Fukushima E, Suzutani T. Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. J Biochem Biophys Methods. 2007;70:499–501.PubMedCrossRef
49.
Nakauchi M, Yoshikawa T, Nakai H, Sugata K, Yoshikawa A, Asano Y, et al. Evaluation of reverse transcription loop-mediated isothermal amplification assays for rapid diagnosis of pandemic influenza A/H1N1 2009 virus. J Med Virol. 2011;83:10–5.PubMedCrossRef
50.
Picard-Meyer E, Barrat J, Cliquet F. Use of filter paper (FTA) technology for sampling, recovery and molecular characterisation of rabies viruses. J Virol Methods 2007;140:174–182
51.
Yamamura M, Makimura K, Ota Y. Evaluation of a new rapid molecular diagnostic system for Plasmodium falciparum combined with DNA filter paper, loop-mediated isothermal amplification, and melting curve analysis. Jpn J Infect Dis. 2009;62:20–5.PubMed
52.
Matovu E, Kuepfer I, Boobo A, Kibona S, Burri C. Comparative detection of trypanosomal DNA by loop-mediated isothermal amplification and PCR from Flinders Technology Associates cards spotted with patient blood. J Clin Microbiol. 2010;48:2087–90.PubMedCrossRef
53.
Asiello PJ, Baeumner AJ. Miniaturized isothermal nucleic acid amplification, a review. Lab Chip. 2011;11:1420–30.PubMedCrossRef
54.
Liu C, Geva E, Mauk M, Qiu X, Abrams WR, Malamud D, et al. An isothermal amplification reactor with an integrated isolation membrane for point-of-care detection of infectious diseases. Analyst. 2011;136:2069–76.PubMedCrossRef
55.
Wu Q, Jin W, Zhou C, Han S, Yang W, Zhu Q, et al. Integrated glass microdevice for nucleic acid purification, loop-mediated isothermal amplification, and online detection. Anal Chem. 2011;83:3336–42.PubMedCrossRef
56.
Wang CH, Lien KY, Wang TY, Chen TY, Lee GB. An integrated microfluidic loop-mediated-isothermal-amplification system for rapid sample pre-treatment and detection of viruses. Biosens Bioelectron. 2011;26:2045–52.PubMedCrossRef
57.
Abe T, Segawa Y, Watanabe H, Yotoriyama T, Kai S, Yasuda A, et al. Point-of-care testing system enabling 30 min detection of influenza genes. Lab Chip. 2011;11:1166–7.PubMedCrossRef
58.
Fang X, Liu Y, Kong J, Jiang X. Loop-mediated isothermal amplification integrated on microfluidic chips for point-of-care quantitative detection of pathogens. Anal Chem. 2010;82:3002–6.PubMedCrossRef
59.
Mori Y, Nagamine K, Tomita N, Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun. 2001;289:150–4.PubMedCrossRef
60.
Tomita N, Mori Y, Kanda H, Notomi T. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc. 2008;3:877–82.PubMedCrossRef
61.
Luo X, Hsing IM. Electrochemical techniques on sequence-specific PCR amplicon detection for point-of-care applications. Analyst. 2009;134:1957–64.PubMedCrossRef
62.
Safavieh M, Ahmed MU, Tolba M, Zourob M. Microfluidic electrochemical assay for rapid detection and quantification of Escherichia coli. Biosens Bioelectron. 2012;31:523–8.PubMedCrossRef
63.
Satoh T, Matsumoto K, Fujii T, Sato O, Gemma N, Onuki M, et al. Rapid genotyping of carcinogenic human papillomavirus by loop-mediated isothermal amplification using a new automated DNA test (Clinichip HPV™). J Virol Methods. 2012;188:83–9.PubMedCrossRef
Metadata
Title
Loop-mediated isothermal amplification (LAMP): recent progress in research and development
Authors
Yasuyoshi Mori
Hidetoshi Kanda
Tsugunori Notomi
Publication date
01-06-2013
Publisher
Springer Japan
Published in
Journal of Infection and Chemotherapy / Issue 3/2013
Print ISSN: 1341-321X
Electronic ISSN: 1437-7780
DOI
https://doi.org/10.1007/s10156-013-0590-0

Other articles of this Issue 3/2013

Journal of Infection and Chemotherapy 3/2013 Go to the issue

Original Article

Minimal biofilm eradication concentration of antimicrobial agents against nontypeable Haemophilus influenzae isolated from middle ear fluids of intractable acute otitis media

Original Article

Nationwide survey of the development of drug-resistant pathogens in the pediatric field in 2007 and 2010: drug sensitivity of Streptococcus pneumoniae in Japan (second report)

Case Report

Usefulness of gram-stained sputum obtained just after administration of antimicrobial agents as the earliest therapeutic indicator for evaluating the effectiveness of empiric therapy in community-acquired pneumonia caused by pneumococcus or Moraxella catarrhalis

Original Article

Good transfer of tebipenem into middle ear effusion conduces to the favorable clinical outcomes of tebipenem pivoxil in pediatric patients with acute otitis media

Original Article

A national survey on myocarditis associated with influenza H1N1pdm2009 in the pandemic and postpandemic season in Japan

Case Report

Bronchitis caused by Bordetella holmesii in a child with asthma misdiagnosed as mycoplasmal infection
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits