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01-12-2024 | Laser | Original Article

Association of Papacarie Duo® and low-level laser in antimicrobial photodynamic therapy (aPDT)

Authors: Roberta Janaina Soares Mendes, Nayanna Matos de Sousa, Guilherme Silva Furtado, Marco Aurélio Benini Paschoal, Andréa Dias Neves Lago

Published in: Lasers in Medical Science | Issue 1/2024

Abstract

Dental caries is a multifactorial, non-communicable disease. Effective treatment options for minimally invasive removal of carious tissue include Papacarie Duo® gel and antimicrobial photodynamic therapy (aPDT). aPDT involves a combination of a light source and photosensitizer. Given that Papacarie Duo® contains a percentage of blue dye, this study aims to explore the antimicrobial potential of Papacarie Duo® when associated with a light source against Streptococcus mutans strains. The chosen light source was a low-power diode laser (λ = 660 nm, E = 3 J, P = 100 mW, t = 30 s). To assess antimicrobial capacity, planktonic suspensions of Streptococcus mutans were plated on Brain Heart Infusion Agar (BHI) to observe the formation of inhibition halos. The studied groups included methylene blue (0.005%), Papacarie Duo®, distilled water (negative control), 2% chlorhexidine (positive control), Papacarie Duo® + laser, and methylene blue (0.005%) + laser. Following distribution onto plates, each group was incubated at 37 °C for 48 h under microaerophilic conditions. Inhibition halos were subsequently measured using a digital caliper. The results showed that chlorhexidine had the greatest antimicrobial effect followed by the group of irradiated methylene blue and irradiated Papacarie Duo®. All experimental groups demonstrated antimicrobial potential, excluding the negative control group. The study concludes that Papacarie Duo® exhibits antimicrobial properties when associated with a low-power diode laser.

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Title: Association of Papacarie Duo® and low-level laser in antimicrobial photodynamic therapy (aPDT)
Authors: Roberta Janaina Soares Mendes
Nayanna Matos de Sousa
Guilherme Silva Furtado
Marco Aurélio Benini Paschoal
Andréa Dias Neves Lago
Publication date: 01-12-2024
Publisher: Springer London
Keywords: Laser
Photodynamic Therapy
Caries
Diode Laser
Published in: Lasers in Medical Science / Issue 1/2024
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-024-03981-9

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Association of Papacarie Duo® and low-level laser in antimicrobial photodynamic therapy (aPDT)

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