Top

Published in:

01-12-2019 | Cytokines | Review Article

Photobiomodulation effects on keratinocytes cultured in vitro: a critical review

Authors: Priscila Thaís Rodrigues de Abreu, José Alcides Almeida de Arruda, Ricardo Alves Mesquita, Lucas Guimarães Abreu, Ivana Márcia Alves Diniz, Tarcília Aparecida Silva

Published in: Lasers in Medical Science | Issue 9/2019

Abstract

Photobiomodulation therapy (PBMT) has been widely used for the promotion of tissue repair. Despite these therapeutic benefits, in some cases, PBMT appears to be unsuccessful, and the strongest hypothesis is that this failure is due to inadequate light dosimetry and wavelengths. The objective of the present critical review was to evaluate the effects of PBMT on cultured keratinocytes using blue, red, or near-infrared light categorized into arbitrary ranges of energy density (0.1–5.0, 5.1–10.0, 10.1–15.0, and over 15.0 J/cm²). The electronic searches were conducted in PubMed, Web of Science, Scopus and LILACS databases, and included LASER or LED devices. A total of 55 articles evaluating the effects of PBMT on cell viability, proliferation, migration, and cytokine and growth factor production were included. Overall, the studies failed to provide detailed information about light dosimetry or detailed experimental conditions. The vast majority of the energy densities tested produced unmodified results regardless of the wavelength applied. However, it was possible to observe that red and near-infrared light had more stimulatory effects than blue light. In addition, for all parameters analyzed, favorable outcomes were mostly obtained in the range of 0.1–5.0 J/cm². The less explored energy densities were within the 10.1–15.0 J/cm² range. Energy densities above 15.0 J/cm² were ineffective or tended to cause cell death. The heterogeneity of the data does not allow us to define a PBMT range setting protocol that would have beneficial effects on keratinocytes.

Available only for authorised users

Diniz IMA, Carreira ACO, Sipert CR, Uehara CM, Moreira MSN, Freire L, Pelissari C, Kossugue PM, de Araújo DR, Sogayar MC, Marques MM (2018) Photobiomodulation of mesenchymal stem cells encapsulated in an injectable rhBMP4-loaded hydrogel directs hard tissue bioengineering. J Cell Physiol 233:4907–4918. https://doi.org/10.1002/jcp.26309 CrossRefPubMed

Elad S, Arany P, Bensadoun RJ, Epstein JB, Barasch A, Raber-Durlacher J (2018) Photobiomodulation therapy in the management of oral mucositis: search for the optimal clinical treatment parameters. Support Care Cancer 26:1–3. https://doi.org/10.1007/s00520-018-4262-6 CrossRef

Engel KW, Khan I, Arany PR (2016) Cell lineage responses to photobiomodulation therapy. J Biophotonics 9:1148–1156. https://doi.org/10.1002/jbio.201600025 CrossRefPubMed

Anders JJ, Lanzafame RJ, Arany PR (2015) Low-level light/laser therapy versus photobiomodulation therapy. Photomed Laser Surg 33:183–184. https://doi.org/10.1089/pho.2015.9848 CrossRefPubMedPubMedCentral

Passarella S, Karu T (2014) Absorption of monochromatic and narrow band radiation in the visible and near IR by both mitochondrial and non-mitochondrial photoacceptors results in photobiomodulation. J Photochem Photobiol B 140:344–358. https://doi.org/10.1016/j.jphotobiol.2014.07.021 CrossRefPubMed

Arany PR, Cho A, Hunt TD, Sidhu G, Shin K, Hahm E, Huang GX, Weaver J, Chen AC, Padwa BL, Hamblin MR, Barcellos-Hoff MH, Kulkarni AB, J Mooney D (2014) Photoactivation of endogenous latent transforming growth factor–β1 directs dental stem cell differentiation for regeneration. Sci Transl Med 6:238ra69. https://doi.org/10.1126/scitranslmed.3008234 CrossRefPubMedPubMedCentral

George S, Hamblin MR, Abrahamse H (2018) Effect of red light and near infrared laser on the generation of reactive oxygen species in primary dermal fibroblasts. J Photochem Photobiol B 188:60–68. https://doi.org/10.1016/j.jphotobiol.2018.09.004 CrossRefPubMedPubMedCentral

Ferreira LS, Diniz IMA, Maranduba CMS, Miyagi SPH, Rodrigues MFSD, Moura-Netto C, Marques MM (2018) Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells. Lasers Med Sci 34(4):659–666. https://doi.org/10.1007/s10103-018-2637-z CrossRefPubMed

Kim JE, Woo YJ, Sohn KM, Jeong KH, Kang H (2017) Wnt/β-catenin and ERK pathway activation: a possible mechanism of photobiomodulation therapy with light-emitting diodes that regulate the proliferation of human outer root sheath cells. Lasers Surg Med 49:940–947. https://doi.org/10.1002/lsm.22736 CrossRefPubMed

10.

Yin K, Zhu R, Wang S, Zhao RC (2017) Low-level laser effect on proliferation, migration, and antiapoptosis of mesenchymal stem cells. Stem Cells Dev 26:762–775. https://doi.org/10.1089/scd.2016.0332 CrossRefPubMed

11.

Karu TI (2010) Multiple roles of cytochrome c oxidase in mammalian cells under action of red and IR-A radiation. IUBMB Life 62:607–610. https://doi.org/10.1002/iub.359 CrossRefPubMed

12.

Hamblin MR (2018) Mechanisms and mitochondrial redox signaling in photobiomodulation. Photochem Photobiol 94:199–212. https://doi.org/10.1111/php.12864 CrossRefPubMedPubMedCentral

13.

Hamblin MR (2017) Mechanisms and applications of the anti-inflammatory effects of photobiomodulation. AIMS Biophys 4:337–361. https://doi.org/10.3934/biophy.2017.3.337 CrossRefPubMedPubMedCentral

14.

Mainster MA (2006) Violet and blue light blocking intraocular lenses: photoprotection versus photoreception. Br J Ophthalmol 90:784–792. https://doi.org/10.1136/bjo.2005.086553 CrossRefPubMedPubMedCentral

15.

Wang Y, Huang YY, Wang Y, Lyu P, Hamblin MR (2017) Red (660 nm) or near-infrared (810 nm) photobiomodulation stimulates, while blue (415 nm), green (540 nm) light inhibits proliferation in human adipose-derived stem cells. Sci Rep 7:7781. https://doi.org/10.1038/s41598-017-07525-w CrossRefPubMedPubMedCentral

16.

da Silva JP, da Silva MA, Almeida APF, Junior IL, Matos AP (2010) Laser therapy in the tissue repair process: a literature review. Photomed Laser Surg 28:17–21. https://doi.org/10.1089/pho.2008.2372 CrossRefPubMed

17.

Basso FG, Soares DG, Pansani TN, Cardoso LM, Scheffel DL, de Souza Costa CA, Hebling J (2016) Proliferation, migration, and expression of oral-mucosal-healing-related genes by oral fibroblasts receiving low-level laser therapy after inflammatory cytokines challenge. Lasers Surg Med 48:1006–1014. https://doi.org/10.1002/lsm.22553 CrossRefPubMed

18.

Houreld NN, Ayuk SM, Abrahamse H (2014) Expression of genes in normal fibroblast cells (WS1) in response to irradiation at 660 nm. J Photochem Photobiol B 130:146–152. https://doi.org/10.1016/j.jphotobiol.2013.11.018 CrossRefPubMed

19.

Rizzi M, Migliario M, Tonello S, Rocchetti V, Renò F (2018) Photobiomodulation induces in vitro re-epithelialization via nitric oxide production. Lasers Med Sci 33:1–6. https://doi.org/10.1007/s10103-018-2443-7 CrossRef

20.

Solmaz H, Ulgen Y, Gulsoy M (2017) Photobiomodulation of wound healing via visible and infrared laser irradiation. Lasers Med Sci 32:903–910. https://doi.org/10.1007/s10103-017-2191-0 CrossRefPubMed

21.

Tata DB, Waynant RW (2011) Laser therapy: a review of its mechanism of action and potential medical applications. Laser Photonics Rev 5:1–12. https://doi.org/10.1002/lpor.200900032 CrossRef

22.

AlGhamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27:237–249. https://doi.org/10.1007/s10103-011-0885-2 CrossRefPubMed

23.

Marques MM, de Cara SPHM, Abe GL, Pedroni ACF, Diniz IMA, Moreira MS (2017) Effects of photobiomodulation therapy in dentoalveolar-derived mesenchymal stem cells: a review of literature. Laser Dent Sci 1:1–7. https://doi.org/10.1007/s41547-017-0002-3 CrossRef

24.

Peplow PV, Chung TY, Baxter GD (2010) Laser photobiomodulation of proliferation of cells in culture: a review of human and animal studies. Photomed Laser Surg Suppl 1:S3–S40. https://doi.org/10.1089/pho.2010.2771 CrossRef

25.

World Association of Laser Therapy (WALT) (2006) Consensus agreement on the design and conduct of clinical studies with low-level laser therapy and light therapy for musculoskeletal pain and disorders. Photomed Laser Surg 24:761–762. https://doi.org/10.1089/pho.2006.24.761 CrossRef

26.

Rooney AA, Boyles AL, Wolfe MS, Bucher JR, Thayer KA (2014) Systematic review and evidence integration for literature-based environmental health science assessments. Environ Health Perspect 122:711–718. https://doi.org/10.1289/ehp.1307972 CrossRefPubMedPubMedCentral

27.

Basso FG, Oliveira CF, Kurachi C, Hebling J, de Souza Costa CA (2013) Biostimulatory effect of low-level laser therapy on keratinocytes in vitro. Lasers Med Sci 28:367–374. https://doi.org/10.1007/s10103-012-1057-8 CrossRefPubMed

28.

Sperandio FF, Simões A, Corrêa L, Aranha AC, Giudice FS, Hamblin MR, Sousa SC (2015) Low-level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair. J Biophotonics 8:795–803. https://doi.org/10.1002/jbio.201400064 CrossRefPubMed

29.

Castellano-Pellicena I, Uzunbajakava NE, Mignon C, Raafs B, Botchkarev VA, Thornton MJ (2018) Does blue light restore human epidermal barrier function via activation of opsin during cutaneous wound healing? Lasers Surg Med 51(4):370–382. https://doi.org/10.1002/lsm.23015 CrossRefPubMed

30.

Walter C, Pabst AM, Ziebart T (2015) Effects of a low-level diode laser on oral keratinocytes, oral fibroblasts, endothelial cells and osteoblasts incubated with bisphosphonates: an in vitro study. Biomed Rep 3:14–18. https://doi.org/10.3892/br.2014.389 CrossRefPubMed

31.

Trachootham D, Alexandre J, Huang P (2009) Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach? Nat Rev Drug Discov 8:579–591. https://doi.org/10.1038/nrd2803 CrossRefPubMed

32.

Gagnon D, Gibson TW, Singh A, zur Linden AR, Kazienko JE, LaMarre J (2016) An in vitro method to test the safety and efficacy of low-level laser therapy (LLLT) in the healing of a canine skin model. BMC Vet Res 12:73. https://doi.org/10.1186/s12917-016-0689-5 CrossRefPubMedPubMedCentral

33.

Lee JB, Bae SH, Moon KR, Na EY, Yun SJ, Lee SC (2016) Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin. Exp Dermatol 25:956–961. https://doi.org/10.1111/exd.13133 CrossRefPubMed

34.

Liebmann J, Born M, Kolb-Bachofen V (2010) Blue-light irradiation regulates proliferation and differentiation in human skin cells. J Invest Dermatol 130:259–269. https://doi.org/10.1038/jid.2009.194 CrossRefPubMed

35.

Migliario M, Rizzi M, Rocchetti V, Cannas M, Renò F (2013) In vitro toxicity of photodynamic antimicrobial chemotherapy on human keratinocytes proliferation. Lasers Med Sci 28:565–569. https://doi.org/10.1007/s10103-012-1112-5 CrossRefPubMed

36.

Szeimies RM, Abels C, Fritsch C, Karrer S, Steinbach P, Bäumler W, Goerz G, Goetz AE, Landthaler M (1995) Wavelength dependency of photodynamic effects after sensitization with 5-aminolevulinic acid in vitro and in vivo. J Invest Dermatol 105:672–677. https://doi.org/10.1111/1523-1747.ep12324377 CrossRefPubMed

37.

Opal SM, Depalo V (2000) Anti-inflammatory cytokines. Chest 117:1162–1172. https://doi.org/10.1378/chest.117.4.1162 CrossRefPubMed

38.

Fushimi T, Inui S, Nakajima T, Ogasawara M, Hosokawa K, Itami S (2012) Green light emitting diodes accelerate wound healing: characterization of the effect and its molecular basis in vitro and in vivo. Wound Repair Regen 20:226–235. https://doi.org/10.1111/j.1524-475X.2012.00771.x CrossRefPubMed

39.

Haas AF, Wong JW, Iwahashi CK, Halliwell B, Cross CE, Davis PA (1998) Redox regulation of wound healing? NF-kappaB activation in cultured human keratinocytes upon wounding and the effect of low energy HeNe irradiation. Free Radic Biol Med 25:998–1005. https://doi.org/10.1016/S0891-5849(98)00135-X CrossRefPubMed

40.

Yu HS, Chang KL, Yu CL, Chen JW, Chen GS (1996) Low-energy helium-neon laser irradiation stimulates interleukin-1 alpha and interleukin-8 release from cultured human keratinocytes. J Invest Dermatol 107:593–596. https://doi.org/10.1111/1523-1747.ep12583090 CrossRefPubMed

41.

Feliciani C, Gupta AK, Saucier DN (1996) Keratinocytes and cytokine/growth factors. Crit Rev Oral Biol Med 7:300–318CrossRef

42.

Baroni A, De Filippis A, Oliviero G, Fusco A, Perfetto B, Buommino E, Donnarumma G (2017) Effect of 1064-nm Q-switched Nd: YAG laser on invasiveness and innate immune response in keratinocytes infected with Candida albicans. Lasers Med Sci 33:941–948. https://doi.org/10.1007/s10103-017-2407-3 CrossRefPubMed

43.

Lim W, Kim J, Lim C, Kim S, Jeon S, Karna S, Cho M, Choi H, Kim O (2012) Effect of 635 nm light-emitting diode irradiation on intracellular superoxide anion scavenging independent of the cellular enzymatic antioxidant system. Photomed Laser Surg 30:451–459. https://doi.org/10.1089/pho.2011.3199 CrossRefPubMed

44.

Mignon C, Uzunbajakava NE, Raafs B, Botchkareva NV, Tobin DJ (2017) Photobiomodulation of human dermal fibroblasts in vitro: decisive role of cell culture conditions and treatment protocols on experimental outcome. Sci Rep 7:2797. https://doi.org/10.1038/s41598-017-02802-0 CrossRefPubMedPubMedCentral

45.

Tucker LD, Lu Y, Dong Y, Yang L, Li Y, Zhao N, Zhang Q (2018) Photobiomodulation therapy attenuates hypoxic-ischemic injury in a neonatal rat model. J Mol Neurosci 65:514–526. https://doi.org/10.1007/s12031-018-1121-3 CrossRefPubMedPubMedCentral

Title: Photobiomodulation effects on keratinocytes cultured in vitro: a critical review
Authors: Priscila Thaís Rodrigues de Abreu
José Alcides Almeida de Arruda
Ricardo Alves Mesquita
Lucas Guimarães Abreu
Ivana Márcia Alves Diniz
Tarcília Aparecida Silva
Publication date: 01-12-2019
Publisher: Springer London
Keywords: Cytokines
Laser
Growth Factors
Growth Factors
Published in: Lasers in Medical Science / Issue 9/2019
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-019-02813-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Photobiomodulation effects on keratinocytes cultured in vitro: a critical review

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 9/2019

Label-free diagnosis of lung cancer with tissue-slice surface-enhanced Raman spectroscopy and statistical analysis

Photobiomodulation-induced analgesia in experimental temporomandibular disorder involves central inhibition of fractalkine

Early intervention with pulse dye and CO2 ablative fractional lasers to improve cutaneous scarring post-lumpectomy: a randomized controlled trial on the impact of intervention on final cosmesis

Fractional carbon dioxide laser and topical tioconazole in the treatment of fingernail onychomycosis

A transient protective effect of low-level laser irradiation against disuse-induced atrophy of rats

Different photodynamic effects of blue light with and without riboflavin on methicillin-resistant Staphylococcus aureus (MRSA) and human keratinocytes in vitro