Top

Published in:

01-11-2009 | Original Article

Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts

Authors: Carla Andreotti Damante, Giorgio De Micheli, Sueli Patrícia Harumi Miyagi, Ilíria Salomão Feist, Márcia Martins Marques

Published in: Lasers in Medical Science | Issue 6/2009

Abstract

The effects of laser phototherapy on the release of growth factors by human gingival fibroblasts were studied in vitro. Cells from a primary culture were irradiated twice (6 h interval), with continuous diode laser [gallium–aluminum–arsenium (GaAlAs), 780 nm, or indium–gallium–aluminum–phosphide (InGaAlP),_660 nm] in punctual and contact mode, 40 mW, spot size 0.042 cm², 3 J/cm² and 5 J/cm² (3 s and 5 s, respectively). Positive [10% fetal bovine serum (FBS)] and negative (1%FBS) controls were not irradiated. Production of keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF) was quantified by enzyme-linked immunosorbent assay (ELISA). The data were statistically compared by analysis of variance (ANOVA) followed by Tukey’s test (P ≤ 0.05). The characterization of the cell line indicated a mesenchymal nature. KGF release was similar in all groups, while that of bFGF was significantly greater (1.49-times) in groups treated with infra-red laser. It was concluded that increased production of bFGF could be one of the mechanisms by which infra-red laser stimulates wound healing.

Pinfildi CE, Liebano RE, Hochman BS, Ferreira LM (2005) Helium-neon laser in viability of random skin flap in rats. Lasers Surg Med 37:74–77. doi:10.1002/lsm.20190 CrossRefPubMed

Yu W, Naim JO, Lanzafame RJ (1997) Effects of photostimulation on wound healing in diabetic mice. Lasers Surg Med 20:56–63. doi:10.1002/(SICI)1096-9101(1997)20:1<56::AID-LSM9>3.0.CO;2-Y CrossRefPubMed

In de Braekt MM, van Alphen FA, Kuijpers-Jagtman AM, Maltha JC (1991) Effect of low level laser therapy on wound healing after palatal surgery in beagle dogs. Lasers Surg Med 11:462–470. doi:10.1002/lsm.1900110512 CrossRefPubMed

Walker MD, Rumpf S, Baxter GD, Hirst DG, Lowe AS (2000) Effect of low-intensity laser irradiation (660 nm) on a radiation-impaired wound-healing model in murine skin. Lasers Surg Med 26:41–47. doi:10.1002/(SICI)1096-9101(2000)26:1<41::AID-LSM7>3.0.CO;2-M CrossRefPubMed

Lowe AS, Walker MD, O’Byrne M, Baxter GD, Hirst DG (1998) Effect of low intensity monochromatic light therapy (890 nm) on a radiation-impaired, wound-healing model in murine skin. Lasers Surg Med 23:291–298. doi:10.1002/(SICI)1096-9101(1998)23:5<291::AID-LSM9>3.0.CO;2-P CrossRefPubMed

Braverman B, McCarthy RJ, Ivankovich AD, Forde DE, Overfield M, Bapna MS (1989) Effect of helium-neon and infrared laser irradiation on wound healing in rabbits. Lasers Surg Med 9:50–58. doi:10.1002/lsm.1900090111 CrossRefPubMed

Hall G, Anneroth G, Schennings T, Zetterqvist L, Rydén H (1994) Effect of low level energy laser irradiation on wound healing. An experimental study in rats. Swed Dent J 18:29–34PubMed

Amorim JC, de Sousa GR, de Barros Silveira L, Prates RA, Pinotti M, Ribeiro MS (2006) Clinical study of the gingiva healing after gingivectomy and low-level laser therapy. Photomed Laser Surg 24:588–594. doi:10.1089/pho.2006.24.588 CrossRefPubMed

Damante CA, Greghi SL, Sant’Ana AC, Passanezi E, Taga R (2004) Histomorphometric study of the healing of human oral mucosa after gingivoplasty and low-level laser therapy. Lasers Surg Med 35:377–384. doi:10.1002/lsm.20111 CrossRefPubMed

10.

Damante CA, Greghi SLA, Sant’Ana ACP, Passanezi E (2004) Clinical evaluation of the effects of low-intensity laser (GaAlAs) on wound healing after gingivoplasty in humans. J Appl Oral Sci 12:133–136. doi:10.1590/S1678-77572004000200010 CrossRef

11.

Mester E, Mester AF, Mester A (1985) The biomedical effects of laser application. Lasers Surg Med 5:31–39. doi:10.1002/lsm.1900050105 CrossRefPubMed

12.

Fernando S, Hill CM, Walker R (1993) A randomised double blind comparative study of low level laser therapy following surgical extraction of lower third molar teeth. Br J Oral Maxillofac Surg 31:170–172. doi:10.1016/0266-4356(93)90118-G CrossRefPubMed

13.

Carrillo JS, Calatayud J, Manso FJ, Barberia E, Martinez JM, Donado M (1990) A randomized double-blind clinical trial on the effectiveness of helium-neon laser in the prevention of pain, swelling and trismus after removal of impacted third molars. Int Dent J 40:31–36PubMed

14.

Ryden H, Persson L, Preber H, Bergstrom J (1994) Effect of low level energy laser irradiation on gingival inflammation. Swed Dent J 18:35–41PubMed

15.

Kreisler M, Christoffers AB, Al-Haj H, Willershausen B, d’Hoedt B (2002) Low level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibroblasts. Lasers Surg Med 30:365–369. doi:10.1002/lsm.10060 CrossRefPubMed

16.

Almeida-Lopes L, Rigau J, Zangaro RA, Guidugli-Neto J, Jaeger MM (2001) Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med 29:179–184. doi:10.1002/lsm.1107 CrossRefPubMed

17.

Pereira AN, Eduardo CP, Matson E, Marques MM (2002) Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. Lasers Surg Med 31:263–267. doi:10.1002/lsm.10107 CrossRefPubMed

18.

Azevedo LH, de Paula Eduardo F, Moreira MS, de Paula Eduardo C, Marques MM (2006) Influence of different power densities of LILT on cultured human fibroblast growth: a pilot study. Lasers Med Sci 21:86–89. doi:10.1007/s10103-006-0379-9 CrossRefPubMed

19.

Marques MM, Pereira AN, Fujihara NA, Nogueira FN, Eduardo CP (2004) Effect of low-power laser irradiation on protein synthesis and ultrastructure of human gingival fibroblasts. Lasers Surg Med 34:260–265. doi:10.1002/lsm.20008 CrossRefPubMed

20.

Fujihara NA, Hiraki KR, Marques MM (2006) Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med 38:332–336. doi:10.1002/lsm.20298 CrossRefPubMed

21.

Eduardo FP, Mehnert DU, Monezi TA et al (2007) Cultured epithelial cells response to phototherapy with low intensity laser. Lasers Surg Med 39:365–372. doi:10.1002/lsm.20481 CrossRefPubMed

22.

Lynch SE (1994) The role of growth factors in periodontal repair and regeneration. In: Periodontal regeneration: current status and direction. Quintessence, St. Louis

23.

Fujisawa K, Miyamoto Y, Nagayama M (2003) Basic fibroblast growth factor and epidermal growth factor reverse impaired ulcer healing of the rabbit oral mucosa. J Oral Pathol Med 32:358–366. doi:10.1034/j.1600-0714.2003.t01-1-00111.x CrossRefPubMed

24.

Oda Y, Kagami H, Ueda M (2004) Accelerating effects of basic fibroblast growth factor on wound healing of rat palatal mucosa. J Oral Maxillofac Surg 62:73–80. doi:10.1016/j.joms.2003.05.007 CrossRefPubMed

25.

Werner S (1998) Keratinocyte growth factor: a unique player in epithelial repair processes. Cytokine Growth Factor Rev 9:153–165. doi:10.1016/S1359-6101(98)00010-0 CrossRefPubMed

26.

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63. doi:10.1016/0022-1759(83)90303-4 CrossRefPubMed

27.

Sanale AR, Firth JD, Uitto VJ, Putnins EE (2002) Keratinocyte growth factor (KGF)-1 and -2 protein and gene expression in human gingival fibroblasts. J Periodontal Res 37:66–74. doi:10.1034/j.1600-0765.2002.t01-1-90770.x PubMed

28.

Campisi J, Morreo G, Pardee AB (1984) Kinetics of G1 transit following brief starvation for serum factors. Exp Cell Res 152:459–466. doi:10.1016/0014-4827(84)90647-5 CrossRefPubMed

29.

Moore P, Ridgway TD, Higbee RG, Howard EW, Lucroy MD (2005) Effect of wavelength on low-intensity laser irradiation-stimulated cell proliferation in vitro. Lasers Surg Med 36:8–12. doi:10.1002/lsm.20117 CrossRefPubMed

30.

Yu W, Naim JO, Lanzafame RJ (1994) The effect of laser irradiation on the release of bFGF from 3T3 fibroblasts. Photochem Photobiol 59:167–170. doi:10.1111/j.1751-1097.1994.tb05017.x CrossRefPubMed

31.

Saygun I, Karacay S, Serdar M, Ural AU, Sencimen M, Kurtis B (2008) Effects of laser irradiation on the release of basic fibroblast growth factor (bFGF), insulin like growth factor-1 (IGF-1), and receptor of IGF-1 (IGFBP3) from gingival fibroblasts. Lasers Med Sci 23:211–215. doi:10.1007/s10103-007-0477-3 CrossRefPubMed

32.

Freshney RI (2005) Culture of animal cells: a manual of basic technique. Wiley-Liss, HobokenCrossRef

33.

McNeil PL, Muthukrishnan L, Warder E, D’Amore PA (1989) Growth factors are released by mechanically wounded endothelial cells. J Cell Biol 109:811–822. doi:10.1083/jcb.109.2.811 CrossRefPubMed

34.

Smith KC (1991) The photobiological basis of low level laser radiation therapy, Stanford University School of Medicine. Laser Ther 3:19–24

Title: Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts
Authors: Carla Andreotti Damante
Giorgio De Micheli
Sueli Patrícia Harumi Miyagi
Ilíria Salomão Feist
Márcia Martins Marques
Publication date: 01-11-2009
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 6/2009
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-008-0582-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 6/2009

Dye-enhanced laser fluorescence detection of caries lesions around brackets

Investigation of common Indian edible salts suitable for kidney disease by laser induced breakdown spectroscopy

Topical imiquimod in conjunction with Nd:YAG laser for tattoo removal

Laser wavelengths and oral implantology

Osteonecrosis of the jaws caused by bisphosphonates: evaluation of a new therapeutic approach using the Er:YAG laser

Comments on: Red light of 647 nm enhances osteogenic differentiation in mesenchymal stem cells