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European Journal of Clinical Microbiology & Infectious Diseases

Published in:

01-08-2021 | Influenza Virus | Brief Report

Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

Authors: Norihito Kaku, Kohji Hashiguchi, Norihiko Akamatsu, Fumiko Wakigawa, Junichi Matsuda, Kenzo Komaru, Takumi Nakao, Yosuke Harada, Atsuko Hara, Naoki Uno, Kei Sakamoto, Yoshitomo Morinaga, Takeshi Kitazaki, Hiroo Hasegawa, Taiga Miyazaki, Masaaki Fukuda, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 8/2021

Abstract

We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.

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Title: Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples
Authors: Norihito Kaku
Kohji Hashiguchi
Norihiko Akamatsu
Fumiko Wakigawa
Junichi Matsuda
Kenzo Komaru
Takumi Nakao
Yosuke Harada
Atsuko Hara
Naoki Uno
Kei Sakamoto
Yoshitomo Morinaga
Takeshi Kitazaki
Hiroo Hasegawa
Taiga Miyazaki
Masaaki Fukuda
Koichi Izumikawa
Hiroshi Mukae
Katsunori Yanagihara
Publication date: 01-08-2021
Publisher: Springer Berlin Heidelberg
Keywords: Influenza Virus
Influenza
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 8/2021
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-021-04193-7

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