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01-12-2020 | Growth Factors | Original Article

Photobiomodulation effect on angiogenic proteins produced and released by dental pulp cells

Authors: Luciana Lourenço Ribeiro Vitor, Mariel Tavares Oliveira Prado Bergamo, Natalino Lourenço-Neto, Vivien Thiemy Sakai, Rodrigo Cardoso Oliveira, Thiago Cruvinel, Daniela Rios, Gustavo Pompermaier Garlet, Carlos Ferreira Santos, Maria Aparecida Andrade Moreira Machado, Thais Marchini Oliveira

Published in: Clinical Oral Investigations | Issue 12/2020

Abstract

Objective

To verify the photobiomodulation effect on angiogenic proteins produced and released by dental human pulpal fibroblasts (HPFs).

Material and methods

HPFs were irradiated with 660-nm low-level laser at fluences of 2.5 J/cm² and 3.7 J/cm². The control group was not irradiated. MTT, crystal violet, and ELISA assays respectively verified viability, proliferation, and angiogenic protein (supernatant/lysate) at 6 h, 12 h, and 24 h after photobiomodulation. Capillary-like structure formation assay verified functional role. Two-way ANOVA/Tukey’s test and ANOVA/Bonferroni’s multiple comparisons test respectively verified cell viability/proliferation and intragroup and intergroup comparisons of protein synthesis (p < 0.05).

Results

Irradiated and non-irradiated HPFs showed statistically similar cell viability and proliferation pattern. Intragroup comparisons showed similar patterns of protein synthesis for all groups: VEGF-A, VEGF-C, and vascular endothelial growth factor receptor 1 (VEGFR1) increased significantly in the supernatant, while FGF-2 and VEGF-A increased significantly in the lysate. The lower fluence significantly increased BMP-9 (6 h) in the supernatant and VEGFR1 (6 h and 12 h) and VEGF-D (24 h) in the lysate, while the higher fluence significantly increased BMP-9 (6 h) in the supernatant and VEGFR1 (12 h) in the lysate. Regardless of the time, both fluences statistically downregulated placental growth factor (PLGF) and PDGF secretion. Both fluences statistically decreased VEGF-A secretion (24 h) and PLGF production (6 h).

Conclusion

Photobiomodulation produced stimulatory effects on angiogenic protein secretion by pulp fibroblasts. In terms of photobiomodulation, over time, both fluences significantly increased the secretion of VEGF-A, VEGF-C, and VEGFR1 and significantly upregulated BMP-9 (6 h) in the supernatant; for capillary-like structure formation, the fluence of 2.5 J/cm² was better than the fluence of 3.7 J/cm².

Clinical relevance

This study results addressed effective photobiomodulation parameters tailored for pulp angiogenesis.

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Title: Photobiomodulation effect on angiogenic proteins produced and released by dental pulp cells
Authors: Luciana Lourenço Ribeiro Vitor
Mariel Tavares Oliveira Prado Bergamo
Natalino Lourenço-Neto
Vivien Thiemy Sakai
Rodrigo Cardoso Oliveira
Thiago Cruvinel
Daniela Rios
Gustavo Pompermaier Garlet
Carlos Ferreira Santos
Maria Aparecida Andrade Moreira Machado
Thais Marchini Oliveira
Publication date: 01-12-2020
Publisher: Springer Berlin Heidelberg
Keywords: Growth Factors
Growth Factors
Laser
Published in: Clinical Oral Investigations / Issue 12/2020
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-020-03298-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Photobiomodulation effect on angiogenic proteins produced and released by dental pulp cells

Abstract

Objective

Material and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Material and methods

Results

Conclusion

Clinical relevance

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