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Clinical Oral Investigations

Published in:

01-12-2013 | Original Article

Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells

Authors: Piero Römer, Josef Köstler, Vasiliki Koretsi, Peter Proff

Published in: Clinical Oral Investigations | Issue 9/2013

Abstract

Objective

This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells.

Materials and methods

In comparison to a control group, three experimental groups were formed from human primary PDL cells stressed with compressive forces, bacterial endotoxins, or a combination of both. Gene expression of cyclooxygenase-2 and RANKL was analysed with RT real-time PCR. The prostaglandin E2 production was determined with ELISA. A co-culture of PDL cells and an osteoclast-progenitor cell line was used in order to demonstrate the osteoclast formation effect caused by the simultaneous combined stress.

Results

The simultaneous combined stress resulted in a 56-fold up-regulation of cyclooxygenase-2 gene expression with a subsequent noticeable rise in the prostaglandin E2 in the culture medium. The RANKL/osteoprotegerin gene expression ratio was 50-fold up-regulated and the osteoclast formation assay revealed 153.5 ± 15.7 tartrate-resistant acid phosphatase (TRAP)-positive cells per well compared with 42.3 ± 3.8 TRAP-positive cells per well of the control group.

Conclusion

The synergistic action of periodontal pathogens and orthodontic forces leads to an increased expression of cyclooxygenase-2 from PDL cells that intensify the RANKL production which in turn induces osteoclast differentiation and subsequent osteoclastogenesis.

Clinical relevance

The present study puts an emphasis on the detrimental effect of orthodontic forces on patients with an active periodontal disease by underlining the significance of cyclooxygenase-2 activity and RANKL binding on the osteoclastogenesis process.

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Title: Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells
Authors: Piero Römer
Josef Köstler
Vasiliki Koretsi
Peter Proff
Publication date: 01-12-2013
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 9/2013
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-013-0928-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells

Abstract

Objective

Materials and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Materials and methods

Results

Conclusion

Clinical relevance

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