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Archives of Virology
Published in: Archives of Virology 2/2016

01-02-2016 | Brief Report

Characterization of siRNAs derived from cucumber green mottle mosaic virus in infected cucumber plants

Authors: Yongqiang Li, Congliang Deng, Qiaoxia Shang, Xiaoli Zhao, Xingliang Liu, Qi Zhou

Published in: Archives of Virology | Issue 2/2016

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Abstract

Virus-derived small interfering RNAs (vsiRNAs) of cucumber green mottle mosaic virus (CGMMV), a member of the genus Tobamovirus, were characterised in cucumber plants by deep sequencing. CGMMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CGMMV-infected cucumber plants. The 5′-terminal base of vsiRNAs was biased towards C/A/U, suggesting that CGMMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were also predicted.
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Metadata
Title
Characterization of siRNAs derived from cucumber green mottle mosaic virus in infected cucumber plants
Authors
Yongqiang Li
Congliang Deng
Qiaoxia Shang
Xiaoli Zhao
Xingliang Liu
Qi Zhou
Publication date
01-02-2016
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 2/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2687-5

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