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Archives of Virology
Published in: Archives of Virology 4/2014

01-04-2014 | Original Article

Molecular variability of cowpea mild mottle virus infecting soybean in Brazil

Authors: L. G. Zanardo, F. N. Silva, A. T. M. Lima, D. F. Milanesi, G. P. Castilho-Urquiza, A. M. R. Almeida, F. M. Zerbini, C. M. Carvalho

Published in: Archives of Virology | Issue 4/2014

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Abstract

Molecular variability was assessed for 18 isolates of cowpea mild mottle virus (CPMMV, genus Carlavirus, family Betaflexiviridae) found infecting soybean in various Brazilian states (Bahia, Goiás, Maranhão, Mato Grosso, Minas Gerais, Pará) in 2001 and 2010. A variety of symptoms was expressed in soybean cv. CD206, ranging from mild (crinkle/blistering leaves, mosaic and vein clearing) to severe (bud blight, dwarfing, leaf and stem necrosis). Recombination analysis revealed only one CPMMV isolate to be recombinant. Pairwise comparisons and phylogenetic analysis were performed for partial genomes (ORF 2 to the 3’ terminus) and for each ORF individually (ORFs 2 to 6), showing the isolates to be distinct. The topology of the phylogenetic tree could be related to symptoms, but not to the year of collection or geographical origin. Additionally, the phylogenetic analysis supported the existence of two distinct strains of the virus, designated CPMMV-BR1 and CPMMV-BR2, with molecular variations between them.
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Metadata
Title
Molecular variability of cowpea mild mottle virus infecting soybean in Brazil
Authors
L. G. Zanardo
F. N. Silva
A. T. M. Lima
D. F. Milanesi
G. P. Castilho-Urquiza
A. M. R. Almeida
F. M. Zerbini
C. M. Carvalho
Publication date
01-04-2014
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 4/2014
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1879-0

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