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01-09-2013 | Original Article

Molecular characterization of five betacryptoviruses infecting four clover species and dill

Authors: Till Lesker, Frank Rabenstein, Edgar Maiss

Published in: Archives of Virology | Issue 9/2013

Abstract

The family Partitiviridae includes plant (Alphacryptovirus and Betacryptovirus), fungal (Partitivirus) and protozoan (Cryspovirus) viruses with bisegmented dsRNA genomes and isometric virions. Cryptic viruses commonly occur in different plant species without causing any symptoms. So far, numerous sequences have been determined for viruses of the genus Alphacryptovirus, but no sequence is available for any assigned member of the genus Betacryptovirus. Following extraction, cloning and sequence analysis of double-stranded RNA in this study, we report the molecular properties of three assigned members of the genus Betacryptovirus, white clover cryptic virus 2, red clover cryptic virus 2 and hop trefoil cryptic virus 2, and two new putative betacryptoviruses found in crimson clover and dill. Betacryptoviruses share sequence motifs with members of the genus Partitivirus. In phylogenetic analyses, members of the genus Betacryptovirus formed a new sub-cluster within the clusters containing members of the genus Partitivirus. Our results provide evidence for a distinct evolutionary lineage of dsRNA viruses of plants and fungi.

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Title: Molecular characterization of five betacryptoviruses infecting four clover species and dill
Authors: Till Lesker
Frank Rabenstein
Edgar Maiss
Publication date: 01-09-2013
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 9/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-013-1691-x

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