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Archives of Virology
Published in: Archives of Virology 9/2012

01-09-2012 | Brief Report

The simian varicella virus ORF A is expressed in infected cells but is non-essential for replication in cell culture

Author: Wayne L. Gray

Published in: Archives of Virology | Issue 9/2012

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Abstract

The simian varicella virus (SVV) genome encodes ORF A, a truncated homolog of SVV ORF 4. The SVV ORF A was expressed as a 1.0-kb transcript in SVV-infected Vero cells. The ORF A promoter was active in infected Vero cells and was stimulated by the SVV immediate-early gene ORF 62 product (IE62), a viral transactivator of SVV genes. The SVV ORF A did not transactivate SVV IE, early, or late gene promoters in transfected Vero cells and was unable to augment IE62-mediated transactivation of SVV promoters. A SVV mutant lacking ORF A replicated as efficiently as wild-type SVV in infected Vero cells, indicating that ORF A expression is not essential for in vitro replication.
Literature
1.
Ferrin LJ, Camerini-Otero RD (1991) Selective cleavage of human DNA: RecA-assisted restriction endonuclease [RARE] cleavage. Science 254:1494–1497PubMedCrossRef
2.
Gray WL (2004) Simian varicella: a model for human varicella-zoster virus infections. Rev Med Virol 14:363–381PubMedCrossRef
3.
Gray WL (2008) Simian varicella in Old World monkeys. Comp Med 58:22–30PubMed
4.
5.
Gray WL, Mahalingam R (2005) A cosmid-based system for inserting mutations and foreign genes into the simian varicella virus genome. J Virol Meth 130:89–94CrossRef
6.
Gray WL, Starnes B, White MW, Mahalingam R (2001) The DNA sequence of the simian varicella virus genome. Virology 284:123–130PubMedCrossRef
7.
Mahalingam R, Gilden DH, Wellish M, Pugazhenthi S (2006) Transactivation of the simian varicella virus (SVV) open reading frame (ORF) 21 promoter by SVV ORF 62 is upregulated in neuronal cells but downregulated in non-neuronal cells by SVV ORF 63 protein. Virology 345:244–250PubMedCrossRef
8.
Mahalingam R, Smith D, Wellish M, Wolf W, Dueland AN, Cohrs R, Soike K, Gilden D (1991) Simian varicella virus DNA in dorsal root ganglia. Proc. Natl Acad Sci USA 88:2750–2752PubMedCrossRef
9.
Mahalingam R, Traina-Dorge V, Wellish M, Lorino R, Sanford R, Ribka EP, Alleman SJ, Brazeau E, Gilden DH (2007) Simian varicella reactivation in cynomolgus monkeys. Virology 368:50–59PubMedCrossRef
10.
Mahalingam R, White T, Wellish M, Gilden D, Gray WL (2000) Sequence analysis of the leftward end of simian varicella virus (EcoRI- I fragment) reveals the presence of an 8-bp repeat flanking the unique long segment and an 881-bp open reading frame that is absent in the varicella-zoster virus genome. Virology 274:420–428PubMedCrossRef
11.
Meyer C, Kerns A, Barron A, Steblow DN, Messaoudi I (2011) Simian varicella virus gene expression during acute and latent infection of rhesus monkeys. J Neurovirol 17:600–612PubMedCrossRef
12.
Moriuchi H, Moriuchi M, Smith HA, Cohen JI (1994) Varicella-zoster virus open reading frame 4 protein is functionally distinct from and does not complement its herpes simplex virus type 1 homolog, ICP27. J Virol 68:1987–1992PubMed
13.
Moriuchi M, Moriuchi H, Debrus D, Piette J, Cohen JI (1995) The acidic amino-terminal region of varicella-zoster virus open reading frame 4 protein is required for transactivation and can functionally replace the corresponding region of herpes simplex virus ICP27. Virology 208:376–382PubMedCrossRef
14.
Ou Y, Gray WL (2006) The simian varicella virus gene 28 and 29 promoters share a common USF binding site and are induced by IE62 transactivation. J Gen Virol 87:1501–1508PubMedCrossRef
15.
Ruyechan WT (2010) Roles of cellular transcription factors in VZV replication. In: Abendroth A, Arvin AM, Moffat JF (eds) Varicella-zoster virus. Springer, Heidelberg, pp 43–65CrossRef
16.
Ruyechan WT, Peng H, Yang M, Hay J (2003) Cellular factors and IE62 activation of VZV promoters. J Med Virol 70:S90–S94PubMedCrossRef
17.
Sato H, Pesnicak L, Cohen JI (2002) Varicella-zoster virus open reading frame 2 encodes a membrane phosphoprotein that is dispensable for viral replication and for establishment of latency. J Virol 76:3575–3578PubMedCrossRef
Metadata
Title
The simian varicella virus ORF A is expressed in infected cells but is non-essential for replication in cell culture
Author
Wayne L. Gray
Publication date
01-09-2012
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 9/2012
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1367-y

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