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Archives of Virology
Published in: Archives of Virology 12/2011

01-12-2011 | Brief Report

Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome

Authors: You Li, Shuai Wang, Hua Zhu, Chunfu Zheng

Published in: Archives of Virology | Issue 12/2011

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Abstract

Herpes simplex virus type 1 (HSV-1) is a ubiquitous human pathogen of skin and mucous membranes. In the present study, the genome of the HSV-1 F strain was cloned as an infectious bacterial artificial chromosome (BAC) clone without any deletions of the viral genes. Additionally, a firefly luciferase cassette was inserted to generate a novel luciferase-expressing HSV-1 BAC. Importantly, the resulting recombinant HSV-1 BAC Luc behaved indistinguishably from the wild-type virus in Vero cells, and the luciferase activity could be easily quantified in vitro. Thus, this novel HSV-1 BAC system would serve as a powerful tool for gene function profiling.
Literature
1.
Azab W, Kato K, Arii J, Tsujimura K, Yamane D, Tohya Y, Matsumura T, Akashi H (2009) Cloning of the genome of equine herpesvirus 4 strain TH20p as an infectious bacterial artificial chromosome. Arch Virol 154:833–842PubMedCrossRef
2.
Borst EM, Hahn G, Koszinowski UH, Messerle M (1999) Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: a new approach for construction of HCMV mutants. J Virol 73:8320–8329PubMed
3.
Cicin-Sain L, Brune W, Bubic I, Jonjic S, Koszinowski UH (2003) Vaccination of mice with bacteria carrying a cloned herpesvirus genome reconstituted in vivo. J Virol 77:8249–8255PubMedCrossRef
4.
Domi A, Moss B (2002) Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells. Proc Natl Acad Sci USA 99:12415–12420PubMedCrossRef
5.
Gierasch WW, Zimmerman DL, Ward SL, Vanheyningen TK, Romine JD, Leib DA (2006) Construction and characterization of bacterial artificial chromosomes containing HSV-1 strains 17 and KOS. J Virol Methods 135:197–206PubMedCrossRef
6.
Heintz N (2001) BAC to the future: the use of bac transgenic mice for neuroscience research. Nat Rev Neurosci 2:861–870PubMedCrossRef
7.
Hirt B (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol 26:365–369PubMedCrossRef
8.
Horsburgh BC, Hubinette MM, Qiang D, MacDonald ML, Tufaro F (1999) Allele replacement: an application that permits rapid manipulation of herpes simplex virus type 1 genomes. Gene Ther 6:922–930PubMedCrossRef
9.
Kanda T, Yajima M, Ahsan N, Tanaka M, Takada K (2004) Production of high-titer Epstein-Barr virus recombinants derived from Akata cells by using a bacterial artificial chromosome system. J Virol 78:7004–7015PubMedCrossRef
10.
Luker GD, Prior JL, Song J, Pica CM, Leib DA (2003) Bioluminescence imaging reveals systemic dissemination of herpes simplex virus type 1 in the absence of interferon receptors. J Virol 77:11082–11093PubMedCrossRef
11.
Luker GD, Leib DA (2005) Luciferase real-time bioluminescence imaging for the study of viral pathogenesis. Methods Mol Biol 292:285–296PubMed
12.
McGregor A, Schleiss MR (2004) Herpesvirus genome mutagenesis by transposon-mediated strategies. Methods Mol Biol 256:281–302PubMed
13.
Meseda CA, Schmeisser F, Pedersen R, Woerner A, Weir JP (2004) DNA immunization with a herpes simplex virus 2 bacterial artificial chromosome. Virology 318:420–428PubMedCrossRef
14.
Rudolph J, O’Callaghan DJ, Osterrieder N (2002) Cloning of the genomes of equine herpesvirus type 1 (EHV-1) strains KyA and racL11 as bacterial artificial chromosomes (BAC). J Vet Med B Infect Dis Vet Public Health 49:31–36PubMedCrossRef
15.
Schumacher D, Tischer BK, Fuchs W, Osterrieder N (2000) Reconstitution of Marek’s disease virus serotype 1 (MDV-1) from DNA cloned as a bacterial artificial chromosome and characterization of a glycoprotein B-negative MDV-1 mutant. J Virol 74:11088–11098PubMedCrossRef
16.
Shizuya H, Kouros-Mehr H (2001) The development and applications of the bacterial artificial chromosome cloning system. Keio J Med 50:26–30PubMedCrossRef
17.
Smith GA, Enquist LW (1999) Construction and transposon mutagenesis in Escherichia coli of a full-length infectious clone of pseudorabies virus, an alphaherpesvirus. J Virol 73:6405–6414PubMed
18.
Suter M, Lew AM, Grob P, Adema GJ, Ackermann M, Shortman K, Fraefel C (1999) BAC-VAC, a novel generation of (DNA) vaccines: a bacterial artificial chromosome (BAC) containing a replication-competent, packaging-defective virus genome induces protective immunity against herpes simplex virus 1. Proc Natl Acad Sci USA 96:12697–12702PubMedCrossRef
19.
Szpara ML, Parsons L, Enquist LW (2010) Sequence variability in clinical and laboratory isolates of herpes simplex virus 1 reveals new mutations. J Virol 84:5303–5313PubMedCrossRef
20.
Tanaka M, Kagawa H, Yamanashi Y, Sata T, Kawaguchi Y (2003) Construction of an excisable bacterial artificial chromosome containing a full-length infectious clone of herpes simplex virus type 1: viruses reconstituted from the clone exhibit wild-type properties in vitro and in vivo. J Virol 77:1382–1391PubMedCrossRef
21.
Terada K, Wakimoto H, Tyminski E, Chiocca EA, Saeki Y (2006) Development of a rapid method to generate multiple oncolytic HSV vectors and their in vivo evaluation using syngeneic mouse tumor models. Gene Ther 13:705–714PubMedCrossRef
22.
Trapp S, Osterrieder N, Keil GM, Beer M (2003) Mutagenesis of a bovine herpesvirus type 1 genome cloned as an infectious bacterial artificial chromosome: analysis of glycoprotein E and G double deletion mutants. J Gen Virol 84:301–306PubMedCrossRef
23.
Van Regenmortel MH, Burke DS, Calisher CH, Dietzgen RG, Fauquet CM, Ghabrial SA, Jahrling PB, Johnson KM, Holbrook MR, Horzinek MC, Keil GM, Kuhn JH, Mahy BW, Martelli GP, Pringle C, Rybicki EP, Skern T, Tesh RB, Wahl-Jensen V, Walker PJ, Weaver SC (2010) A proposal to change existing virus species names to non-Latinized binomials. Arch Virol 155:1909–1919PubMedCrossRef
24.
Wang J, Osterrieder N (2011) Generation of an infectious clone of duck enteritis virus (DEV) and of a vectored DEV expressing hemagglutinin of H5N1 avian influenza virus. Virus Res 159:23–31PubMedCrossRef
25.
Warden C, Tang Q, Zhu H (2011) Herpesvirus BACs: past, present, and future. J Biomed Biotechnol 2011:124595PubMedCrossRef
26.
Yu D, Smith GA, Enquist LW, Shenk T (2002) Construction of a self-excisable bacterial artificial chromosome containing the human cytomegalovirus genome and mutagenesis of the diploid TRL/IRL13 gene. J Virol 76:2316–2328PubMedCrossRef
27.
Zhang Z, Rowe J, Wang W, Sommer M, Arvin A, Moffat J, Zhu H (2007) Genetic analysis of varicella-zoster virus ORF0 to ORF4 by use of a novel luciferase bacterial artificial chromosome system. J Virol 81:9024–9033PubMedCrossRef
28.
Zhang Z, Huang Y, Zhu H (2010) An efficient protocol for VZV BAC-based mutagenesis. Methods Mol Biol 634:75–86PubMedCrossRef
29.
Zhang Z, Selariu A, Warden C, Huang G, Huang Y, Zaccheus O, Cheng T, Xia N, Zhu H (2010) Genome-wide mutagenesis reveals that ORF7 is a novel VZV skin-tropic factor. PLoS Pathog 6:e1000971PubMedCrossRef
Metadata
Title
Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome
Authors
You Li
Shuai Wang
Hua Zhu
Chunfu Zheng
Publication date
01-12-2011
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 12/2011
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-011-1094-9

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