Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 6/2011

01-06-2011 | Brief Report

Generation and characterization of koi herpesvirus recombinants lacking viral enzymes of nucleotide metabolism

Authors: Walter Fuchs, Dieter Fichtner, Sven M. Bergmann, Thomas C. Mettenleiter

Published in: Archives of Virology | Issue 6/2011

Login to get access

Abstract

Koi herpesvirus (KHV) causes a fatal disease in koi and common carp, but no reliable and genetically characterized vaccines are available up to now. Therefore, we generated KHV recombinants possessing deletions within the viral ribonucleotide reductase (RNR), thymidine kinase (TK), dUTPase, or TK and dUTPase genes, and their corresponding rescuants. All KHV mutants were replication competent in cultured cells. Whereas plaque sizes and titers of RNR-negative KHV were reduced, replication of the other mutants was not affected. Experimental infection of carp indicated attenuation of TK- or dUTPase-deleted KHV, and PCR analysis of tissue samples permitted differentiation of mutant from wild-type virus.
Appendix
Available only for authorised users
Literature
1.
Aoki T, Hirono I, Kurokawa K, Fukuda H, Nahary R, Eldar A, Davison AJ, Waltzek TB, Bercovier H, Hedrick RP (2007) Genome sequences of three koi herpesvirus isolates representing the expanding distribution of an emerging disease threatening koi and common carp worldwide. J Virol 81:5058–5065PubMedCrossRef
2.
Bercovier H, Fishman Y, Nahary R, Sinai S, Zlotkin A, Eyngor M, Gilad O, Eldar A, Hedrick RP (2005) Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis. BMC Microbiol 5:13PubMedCrossRef
3.
Bigarré L, Baud M, Cabon J, Antychowicz J, Bergmann SM, Engelsma M, Pozet F, Reichert M, Castric J (2009) Differentiation between Cyprinid herpesvirus type-3 lineages using duplex PCR. J Virol Methods 158:51–57PubMedCrossRef
4.
Bretzinger A, Fischer-Scherl T, Oumouna M, Hoffmann R, Truyen U (1999) Mass mortalities in koi carp, Cyprinus carpio, associated with gill and skin disease. Bull Eur Assoc Fish Pathol 19:182–185
5.
Costes B, Fournier G, Michel B, Delforge C, Raj VS, Dewals B, Gillet L, Drion P, Body A, Schynts F, Lieffrig F, Vanderplasschen A (2008) Cloning of the koi herpesvirus genome as an infectious bacterial artificial chromosome demonstrates that disruption of the thymidine kinase locus induces partial attenuation in Cyprinus carpio koi. J Virol 82:4955–4964PubMedCrossRef
6.
Davison AJ, Eberle R, Ehlers B, Hayward GS, McGeoch DJ, Minson AC, Pellett PE, Roizman B, Studdert MJ, Thiry E (2009) The order Herpesvirales. Arch Virol 154:171–177PubMedCrossRef
7.
de Wind N, Berns A, Gielkens A, Kimman T (1993) Ribonucleotide reductase-deficient mutants of pseudorabies virus are avirulent for pigs and induce partial protective immunity. J Gen Virol 74:351–359PubMedCrossRef
8.
Efstathiou S, Kemp S, Darby G, Minson AC (1989) The role of herpes simplex virus type 1 thymidine kinase in pathogenesis. J Gen Virol 70:869–879PubMedCrossRef
9.
Fuchs W, Mettenleiter TC (1996) DNA sequence and transcriptional analysis of the UL1 to UL5 gene cluster of infectious laryngotracheitis virus. J Gen Virol 77:2221–2229PubMedCrossRef
10.
Fuchs W, Mettenleiter TC (1999) DNA sequence of the UL6 to UL20 genes of infectious laryngotracheitis virus and characterization of the UL10 gene product as a nonglycosylated and nonessential virion protein. J Gen Virol 80:2173–2182PubMed
11.
Fuchs W, Ziemann K, Teifke JP, Werner O, Mettenleiter TC (2000) The non-essential UL50 gene of avian infectious laryngotracheitis virus encodes a functional dUTPase which is not a virulence factor. J Gen Virol 81:627–638PubMed
12.
Gilad O, Yun S, Andree KB, Adkison MA, Zlotkin A, Bercovier H, Eldar A, Hedrick RP (2002) Initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, Cyprinus carpio koi. Dis Aquat Organ 48:101–108PubMedCrossRef
13.
Gilad O, Yun S, Adkison MA, Way K, Willits NH, Bercovier H, Hedrick RP (2003) Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus, and the effect of water temperature on mortality of experimentally infected koi. J Gen Virol 84:2661–2668PubMedCrossRef
14.
Gilad O, Yun S, Zagmutt-Vergara FJ, Leutenegger CM, Bercovier H, Hedrick RP (2004) Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR. Dis Aquat Organ 60:179–187PubMedCrossRef
15.
Goldstein DJ, Weller SK (1988) Herpes simplex virus type 1-induced ribonucleotide reductase activity is dispensable for virus growth and DNA synthesis: isolation and characterization of an ICP6 lacZ insertion mutant. J Virol 62:196–205PubMed
16.
Hedrick RP, Gilad O, Yun S, Spangenberg JV, Marty GD, Nordhausen RW, Kebus MJ, Bercovier H, Eldar A (2000) A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of common carp. J Aquat Anim Health 12:44–57CrossRef
17.
Heineman TC, Cohen JI (1994) Deletion of the varicella-zoster virus large subunit of ribonucleotide reductase impairs growth of virus in vitro. J Virol 68:3317–3323PubMed
18.
Jacobson JG, Leib DA, Goldstein DJ, Bogard CL, Schaffer PA, Weller SK, Coen DM (1989) A herpes simplex virus ribonucleotide reductase deletion mutant is defective for productive acute and reactivatable latent infections of mice and for replication in mouse cells. Virology 173:276–283PubMedCrossRef
19.
Jöns A, Mettenleiter TC (1996) Identification and characterization of pseudorabies virus dUTPase. J Virol 70:1242–1245PubMed
20.
Jöns A, Gerdts V, Lange E, Kaden V, Mettenleiter TC (1997) Attenuation of dUTPase-deficient pseudorabies virus for the natural host. Vet Microbiol 56:47–54PubMedCrossRef
21.
Kit S (1989) Recombinant-derived modified-live herpesvirus vaccines. Adv Exp Med Biol 251:219–236PubMed
22.
Neukirch M, Böttcher K, Bunnajirakul S (1999) Isolation of a virus from koi with altered gills. Bull Eur Assoc Fish Pathol 19:221–224
23.
Perelberg A, Ronen A, Hutoran M, Smith Y, Kotler M (2005) Protection of cultured Cyprinus carpio against a lethal viral disease by an attenuated virus vaccine. Vaccine 23:3396–3403PubMedCrossRef
24.
Perelberg A, Ilouze M, Kotler M, Steinitz M (2008) Antibody response and resistance of Cyprinus carpio immunized with cyprinid herpes virus 3 (CyHV-3). Vaccine 26:3750–3756PubMedCrossRef
25.
Ronen A, Perelberg A, Abramowitz J, Hutoran M, Tinman S, Bejerano I, Steinitz M, Kotler M (2003) Efficient vaccine against the virus causing a lethal disease in cultured Cyprinus carpio. Vaccine 21:4677–4684PubMedCrossRef
26.
Rosenkranz D, Klupp BG, Teifke JP, Granzow H, Fichtner D, Mettenleiter TC, Fuchs W (2008) Identification of envelope protein pORF81 of koi herpesvirus. J Gen Virol 89:896–900PubMedCrossRef
27.
Schnitzlein WM, Winans R, Ellsworth S, Tripathy DN (1995) Generation of thymidine kinase-deficient mutants of infectious laryngotracheitis virus. Virology 209:304–314PubMedCrossRef
28.
Zhang HG, Hanson LA (1995) Deletion of thymidine kinase gene attenuates channel catfish herpesvirus while maintaining infectivity. Virology 209:658–663PubMedCrossRef
Metadata
Title
Generation and characterization of koi herpesvirus recombinants lacking viral enzymes of nucleotide metabolism
Authors
Walter Fuchs
Dieter Fichtner
Sven M. Bergmann
Thomas C. Mettenleiter
Publication date
01-06-2011
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2011
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-011-0953-8

Other articles of this Issue 6/2011

Archives of Virology 6/2011 Go to the issue

Original Article

A large outbreak of hand, foot, and mouth disease caused by EV71 and CAV16 in Guangdong, China, 2009

Original Article

Atypical characteristics of nucleoprotein of pandemic influenza virus H1N1 and their roles in reassortment restriction

Brief Report

One-year molecular survey of astrovirus infection in turkeys in Poland

Annotated Sequence Record

Discovery of a genome of a distant relative of chicken anemia virus reveals a new member of the genus Gyrovirus

Annotated Sequence Record

The complete genome sequence and genome structure of passion fruit mosaic virus

Brief Report

Complete nucleotide sequence of a Spanish isolate of alfalfa mosaic virus: evidence for additional genetic variability
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits