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Archives of Virology
Published in: Archives of Virology 6/2009

01-06-2009 | Original Article

A clinical isolate of dengue virus and its proteins induce apoptosis in HMEC-1 cells: a possible implication in pathogenesis

Authors: Melina Vásquez Ochoa, Julio García Cordero, Benito Gutiérrez Castañeda, Leopoldo Santos Argumedo, Nicolas Villegas Sepúlveda, Leticia Cedillo Barrón

Published in: Archives of Virology | Issue 6/2009

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Abstract

Cumulative studies have demonstrated that dengue virus infection results in the induction of apoptosis of certain cells in vitro. Moreover, apoptosis of microvascular endothelial cells in the brain and in the intestinal serosa has been demonstrated postmortem in dengue virus (DENV)-infected patients. In this work, human microvascular endothelial cells (HMEC-1) infected with a DENV-2 clinical isolate, or HMEC-1 cells transfected with its protease sequence (NS3pro) or its complex (NS2BNS3pro) were able to trigger apoptosis after 24 h of infection or transfection. The infected or transfected HMEC-1 cells displayed the distinctive apoptotic hallmarks, which include cytoplasmic shrinkage and plasma membrane blebbing. In addition, the transfected HMEC-1 cells showed biochemical changes such as exposure of phosphatidylserine on the outer leaflet of the plasma membrane, TUNEL positivity, caspase 3 activation and cleaved PARP, a central regulator of apoptosis. These findings suggest the role of such proteins from the clinical isolate in the induction of apoptosis.
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Metadata
Title
A clinical isolate of dengue virus and its proteins induce apoptosis in HMEC-1 cells: a possible implication in pathogenesis
Authors
Melina Vásquez Ochoa
Julio García Cordero
Benito Gutiérrez Castañeda
Leopoldo Santos Argumedo
Nicolas Villegas Sepúlveda
Leticia Cedillo Barrón
Publication date
01-06-2009
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2009
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-009-0396-7

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