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Archives of Virology
Published in: Archives of Virology 12/2008

01-12-2008 | Brief Report

Efficient production of type 2 porcine circovirus-like particles by a recombinant baculovirus

Authors: Lan-Jun Liu, Takako Suzuki, Hiroshi Tsunemitsu, Michiyo Kataoka, Noriyo Ngata, Naokazu Takeda, Takaji Wakita, Tatsuo Miyamura, Tian-Cheng Li

Published in: Archives of Virology | Issue 12/2008

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Abstract

The capsid protein of PCV2 was expressed by using a recombinant baculovirus with insect Tn5 cells. A large amount of 28-kDa protein was released into the culture medium and self-assembled into PCV2-like particles (PCV2-LPs) with a buoyant density of 1.365 g/cm3 and a diameter of 20 nm. PCV2-LPs were efficiently expressed, yielding 1 mg of purified particles per 107 Tn5 cells. The PCV2-LPs have antigenicity similar to that of authentic PCV2 particles, allowing us to develop a method for sensitively detecting PCV2-specific IgG antibodies. In addition, the PCV2-LPs appeared to be the most promising PCV2 vaccine candidate, by virtue of their potent immunogenicity.
Literature
1.
Allan GM, Ellis JA (2000) Porcine circoviruses: a review. J Vet Diagn Invest 12:3–14PubMed
2.
Allan GM, Mc Neilly F, Meehan BM, Kennedy S, Mackie DP, Ellis JA, Clark EG, Espuna E, Saubi N, Riera P, Botner A, Charreyre CE (1999) Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Vet Microbiol 66:115–123PubMedCrossRef
3.
Baumert TF, Vergalla J, Satoi J, Thomson M, Lechmann M, Herion D, Greenberg HB, Ito S, Liang TJ (1999) Hepatitis C virus-like particles synthesized in insect cells as a potential vaccine candidate. Gastroenterology 117:1397–1407PubMedCrossRef
4.
Blanchard P, Mahe D, Cariolet R, Keranflec’h A, Baudouard MA, Cordioli P, Albina E, Jestin A (2003) Protection of swine against post-weaning multisystemic wasting syndrome (PMWS) by porcine circovirus type 2 (PCV2) proteins. Vaccine 21:4565–4575PubMedCrossRef
5.
Ellis J, Hassard L, Clark E, Harding J, Allan G, Willson P, Strokappe J, Martin K, McNeilly F, Meehan B, Todd D, Haines D (1998) Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can Vet J 39:44–51PubMed
6.
Ellis J, Krakowka S, Lairmore M, Haines D, Bratanich A, Clark E, Allan G, Konoby C, Hassard L, Meehan B, Martin K, Harding J, Kennedy S, McNeilly F (1999) Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. J Vet Diagn Invest 11:3–14PubMed
7.
Ellis JA, Bratanich A, Clark EG, Allan G, Meehan B, Haines DM, Harding J, West KH, Krakowka S, Konoby C, Hassard L, Martin K, McNeilly F (2000) Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome. J Vet Diagn Invest 12:21–27PubMed
8.
Fenaux M, Halbur PG, Gill M, Toth TE, Meng XJ (2000) Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38:2494–2503PubMed
9.
Fenaux M, Opriessnig T, Halbur PG, Elvinger F, Meng XJ (2004) A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. J Virol 78:6297–6303PubMedCrossRef
10.
Hasslung F, Wallgren P, Ladekjaer-Hansen AS, Botner A, Nielsen J, Wattrang E, Allan GM, McNeilly F, Ellis J, Timmusk S, Belak K, Segall T, Melin L, Berg M, Fossum C (2005) Experimental reproduction of postweaning multisystemic wasting syndrome (PMWS) in pigs in Sweden and Denmark with a Swedish isolate of porcine circovirus type 2. Vet Microbiol 106:49–60PubMedCrossRef
11.
Ishii Y, Ozaki S, Tanaka K, Kanda T (2005) Human papillomavirus 16 minor capsid protein L2 helps capsomeres assemble independently of intercapsomeric disulfide bonding. Virus Genes 31:321–328PubMedCrossRef
12.
Kim J, Chung HK, Jung T, Cho WS, Choi C, Chae C (2002) Postweaning multisystemic wasting syndrome of pigs in Korea: prevalence, microscopic lesions and coexisting microorganisms. J Vet Med Sci 64:57–62PubMedCrossRef
13.
Lekcharoensuk P, Morozov I, Paul PS, Thangthumniyom N, Wajjawalku W, Meng XJ (2004) Epitope mapping of the major capsid protein of type 2 porcine circovirus (PCV2) by using chimeric PCV1 and PCV2. J Virol 78:8135–8145PubMedCrossRef
14.
Li TC, Yamakawa Y, Suzuki K, Tatsumi M, Razak MA, Uchida T, Takeda N, Miyamura T (1997) Expression and self-assembly of empty virus-like particles of hepatitis E virus. J Virol 71:7207–7213PubMed
15.
Li TC, Takeda N, Kato K, Nilsson J, Xing L, Haag L, Cheng RH, Miyamura T (2003) Characterization of self-assembled virus-like particles of human polyomavirus BK generated by recombinant baculoviruses. Virology 311:115–124PubMedCrossRef
16.
Li TC, Takeda N, Miyamura T, Matsuura Y, Wang JC, Engvall H, Hammar L, Xing L, Cheng RH (2005) Essential elements of the capsid protein for self-assembly into empty virus-like particles of hepatitis E virus. J Virol 79:12999–13006PubMedCrossRef
17.
Mahe D, Blanchard P, Truong C, Arnauld C, Le Cann P, Cariolet R, Madec F, Albina E, Jestin A (2000) Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes. J Gen Virol 81:1815–1824PubMed
18.
Matsuo E, Tani H, Lim C, Komoda Y, Okamoto T, Miyamoto H, Moriishi K, Yagi S, Patel AH, Miyamura T, Matsuura Y (2006) Characterization of HCV-like particles produced in a human hepatoma cell line by a recombinant baculovirus. Biochem Biophys Res Commun 340:200–208PubMedCrossRef
19.
McNeilly F, McNair I, Mackie DP, Meehan BM, Kennedy S, Moffett D, Ellis J, Krakowka S, Allan GM (2001) Production, characterisation and applications of monoclonal antibodies to porcine circovirus 2. Arch Virol 146:909–922PubMedCrossRef
20.
Meehan BM, McNeilly F, Todd D, Kennedy S, Jewhurst VA, Ellis JA, Hassard LE, Clark EG, Haines DM, Allan GM (1998) Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. J Gen Virol 79(Pt 9):2171–2179PubMed
21.
Meerts P, Misinzo G, Lefebvre D, Nielsen J, Botner A, Kristensen CS, Nauwynck HJ (2006) Correlation between the presence of neutralizing antibodies against porcine circovirus 2 (PCV2) and protection against replication of the virus and development of PCV2-associated disease. BMC Vet Res 2:6PubMedCrossRef
22.
Nawagitgul P, Morozov I, Bolin SR, Harms PA, Sorden SD, Paul PS (2000) Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J Gen Virol 81:2281–2287PubMed
23.
Onuki A, Abe K, Togashi K, Kawashima K, Taneichi A, Tsunemitsu H (1999) Detection of porcine circovirus from lesions of a pig with wasting disease in Japan. J Vet Med Sci 61:1119–1123PubMedCrossRef
24.
Pogranichnyy RM, Yoon KJ, Harms PA, Swenson SL, Zimmerman JJ, Sorden SD (2000) Characterization of immune response of young pigs to porcine circovirus type 2 infection. Viral Immunol 13:143–153PubMed
25.
Pringle CR (1999) Virus taxonomy-1999. The universal system of virus taxonomy, updated to include the new proposals ratified by the International Committee on Taxonomy of Viruses during 1998. Arch Virol 144:421–429PubMedCrossRef
26.
Rodriguez-Arrioja GM, Segales J, Calsamiglia M, Resendes AR, Balasch M, Plana-Duran J, Casal J, Domingo M (2002) Dynamics of porcine circovirus type 2 infection in a herd of pigs with postweaning multisystemic wasting syndrome. Am J Vet Res 63:354–357PubMedCrossRef
27.
Wang X, Jiang P, Li Y, Jiang W, Dong X (2007) Protection of pigs against post-weaning multisystemic wasting syndrome by a recombinant adenovirus expressing the capsid protein of porcine circovirus type 2. Vet Microbiol 121:215–224PubMedCrossRef
Metadata
Title
Efficient production of type 2 porcine circovirus-like particles by a recombinant baculovirus
Authors
Lan-Jun Liu
Takako Suzuki
Hiroshi Tsunemitsu
Michiyo Kataoka
Noriyo Ngata
Naokazu Takeda
Takaji Wakita
Tatsuo Miyamura
Tian-Cheng Li
Publication date
01-12-2008
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 12/2008
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-008-0248-x

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