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Published in: Archives of Virology 11/2008

01-11-2008 | Brief Report

Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses

Authors: Andor Doszpoly, Endre R. Kovács, Giuseppe Bovo, Scott E. LaPatra, Balázs Harrach, Mária Benkő

Published in: Archives of Virology | Issue 11/2008

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Abstract

A PCR method with consensus degenerate primers was developed for the detection of herpesviruses (HVs) of anamnia. Compared to previously published PCRs, targeting the DNA polymerase gene of fish HVs, the size of PCR products was more than tripled. Although broad applicability of the method could not be proven, approximately 1,600-bp fragments from HVs of white sturgeon (Acipenser transmontanus) and black bullhead (Ameiurus melas) were obtained and sequenced. Phylogenetic tree reconstructions showed both HVs to be monophyletic with the single member (ictalurid HV-1) of the genus Ictalurivirus in the new family Alloherpesviridae.
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Metadata
Title
Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses
Authors
Andor Doszpoly
Endre R. Kovács
Giuseppe Bovo
Scott E. LaPatra
Balázs Harrach
Mária Benkő
Publication date
01-11-2008
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 11/2008
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-008-0230-7

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