Top

Published in:

01-09-2015 | Original Article

Stereological evaluation of testes in busulfan-induced infertility of hamster

Authors: Mohadeseh Panahi, Negar Karimaghai, Farhad Rahmanifar, Amin Tamadon, Akbar Vahdati, Davood Mehrabani, Omid Koohi-Hosseinabadi, Masood Sepehrimanesh

Published in: Comparative Clinical Pathology | Issue 5/2015

Abstract

Cytotoxic medications can influence spermatogenesis at least temporarily and in some cases permanently. Busulfan is a cytotoxic bifunctional alkylating agent which inhibits cell division by sticking to DNA strands. This study aims to stereologically evaluate the testes in busulfan-induced infertility of azoospermic albino hamsters. Eighteen male adult albino hamsters were randomly divided into 3 equal groups. The first group received just one dose of busulfan (10 mg/kg, intraperitoneally, Busilvex^®, France), the second group received two doses of busulfan with 21-days interval, and the control group left identically without any busulfan therapy. On day 35 postinjection, the testes of all animals were removed for histological evaluation and also for stereological indices. Lumen, cellular and total diameters, luminal, cellular and cross-sectional areas, number of tubules per unit area of testis, numerical density of the tubules, and spermatogenesis index in hamsters that received two doses of busulfan were significantly more than the hamsters receiving one dose of busulfan and the control group. Two doses of busulfan injected with 21-days interval could properly induce azoospermia in the experimental hamster model.

Anjamrooz SH, Movahedin M, Mowla SJ, Bairanvand SP (2007) Assessment of morphological and functional changes in the mouse testis and epididymal sperms following busulfan treatment. Iran Biomed J 11:15–22PubMed

Bucci LR, Meistrich ML (1987) Effects of busulfan on murine spermatogenesis: cytotoxicity, sterility, sperm abnormalities, and dominant lethal mutations. Mutat Res Fund Mol Mech Mut 176:259–268CrossRef

Chang CL-T, Fung H-P, Lin Y-F, Kuo C-Y, Chien C-W (2002) Indenopyridine hydrochloride induced testicular spermatogenesis failure with high seminal alkaline phosphatase levels in male dog. Biol Pharm Bull 25:1097–1100CrossRefPubMed

Choi Y-J, Ok D-W, Kwon D-N, Chung J-I, Kim H-C, Yeo S-M, Kim T, Seo H-G, Kim J-H (2004) Murine male germ cell apoptosis induced by busulfan treatment correlates with loss of c-kit-expression in a Fas/FasL-and p53-independent manner. FEBS Lett 575:41–51CrossRefPubMed

de Rooij DG, Vergouwen RP (1990) The estimation of damage to testicular cell lineages. Prog Clin Biol Res 372:467–480

Dohle GR (2010) Male infertility in cancer patients: review of the literature. Int J Urol 17:327–331CrossRefPubMed

Gilliland KO, Freel CD, Lane CW, Fowler WC, Costello MJ (2001) Multilamellar bodies as potential scattering particles in human age-related nuclear cataracts. Mol Vis 7:120–130PubMed

Gundersen HJG (1977) Notes on the estimation of the numerical density of arbitrary profiles: the edge effect. J Microsc 111:219–223CrossRef

Gundersen HJG, Jensen EB (1987) The efficiency of systematic sampling in stereology and its prediction. J Microsc 147:229–263CrossRefPubMed

Iwamoto T, Hiraku Y, Oikawa S, Mizutani H, Kojima M, Kawanishi S (2004) DNA intrastrand cross-link at the 5′-GA-3′ sequence formed by busulfan and its role in the cytotoxic effect. Cancer Sci 95:454–458CrossRefPubMed

Jiang FX (1998) Behaviour of spermatogonia following recovery from busulfan treatment in the rat. Anat Embryol (Berl) 198:53–61CrossRef

Kramer MF, De Rooij DG (1969) The effect of three alkylating agents on the seminiferous epithelium of rodents. Virchows Archiv B 4:276–282

Krause VW (1975) Teratogenic effect of busulfan on testis cells of the rat (postnatal development). Arzneimittelforschung 25:644PubMed

Krivoy N, Hoffer E, Lurie Y, Bentur Y, Rowe JM (2008) Busulfan use in hematopoietic stem cell transplantation: pharmacology, dose adjustment, safety and efficacy in adults and children. Curr Drug Saf 3:60–66CrossRefPubMed

Le Bourgeois A, Lestang E, Guillaume T, Delaunay J, Ayari S, Blin N, Clavert A, Tessoulin B, Dubruille V, Mahe B, Roland V, Gastinne T, Le Gouill S, Moreau P, Mohty M, Planche L, Chevallier P (2013) Prognostic impact of immune status and hematopoietic recovery before and after fludarabine, IV busulfan, and antithymocyte globulins (FB2 regimen) reduced-intensity conditioning regimen (RIC) allogeneic stem cell transplantation (allo-SCT). Eur J Haematol 90:177–186CrossRefPubMed

Nieto Y, Thall P, Valdez B, Andersson B, Popat U, Anderlini P, Shpall EJ, Bassett R, Alousi A, Hosing C, Kebriaei P, Qazilbash M, Frazier E, Gulbis A, Chancoco C, Bashir Q, Ciurea S, Khouri I, Parmar S, Shah N, Worth L, Rondon G, Champlin R, Jones RB (2012) High-dose infusional gemcitabine combined with busulfan and melphalan with autologous stem-cell transplantation in patients with refractory lymphoid malignancies. Biol Blood Marrow Transplant 18:1677–1686PubMedCentralCrossRefPubMed

Panahi M, Keshavarz S, Rahmanifar F, Tamadon A, Mehrabani D, Karimaghai N, Sepehrimanesh M, Aqababa H (2015) Busulfan induced azoospermia: stereological evaluation of testes in rat. Vet Res Forum. In press

Suttorp M, Millot F (2010) Treatment of pediatric chronic myeloid leukemia in the year 2010: use of tyrosine kinase inhibitors and stem-cell transplantation. ASH Education Program Book 2010:368–376

Wallace WHB, Anderson RA, Irvine DS (2005) Fertility preservation for young patients with cancer: who is at risk and what can be offered? Lancet Oncol 6:209–218CrossRefPubMed

Wang DZ, Zhou XH, Yuan YL, Zheng XM (2010) Optimal dose of busulfan for depleting testicular germ cells of recipient mice before spermatogonial transplantation. Asian J Androl 12:263–270PubMedCentralCrossRefPubMed

Whitsett JM, Noden PF, Cherry J, Lawton AD (1984) Effect of transitional photoperiods on testicular development and puberty in male deer mice (Peromyscus maniculatus). J Reprod Fertil 72:277–286CrossRefPubMed

Zhang Z, Shao S, Meistrich ML (2006) Irradiated mouse testes efficiently support spermatogenesis derived from donor germ cells of mice and rats. J Androl 27:365–375CrossRefPubMed

Title: Stereological evaluation of testes in busulfan-induced infertility of hamster
Authors: Mohadeseh Panahi
Negar Karimaghai
Farhad Rahmanifar
Amin Tamadon
Akbar Vahdati
Davood Mehrabani
Omid Koohi-Hosseinabadi
Masood Sepehrimanesh
Publication date: 01-09-2015
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 5/2015
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-014-2029-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Stereological evaluation of testes in busulfan-induced infertility of hamster

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 5/2015

A case of chronic lymphoblastic leukemia with an abnormal rare karyotype

Normal haematological and blood biochemistry values of cultured Heteroclarias hybrid in South East Nigeria

Effect of different anesthetic agents (clove oil, tricaine methanesulfonate, ketamine, tobacco) on hematological parameters and stress indicators of rainbow trout Oncorhynchus mykiss, Walbaum, 1792

Pyothorax associated with Salmonella and Pseudomonas spp. infection in a FIV-positive cat

Hematology of three age groups of captive Chinese alligator during deep and late hibernation

Isolation, culture, characterization, and adipogenic differentiation of heifer endometrial mesenchymal stem cells