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Published in: Comparative Clinical Pathology 5/2015

01-09-2015 | Original Article

Effect of different anesthetic agents (clove oil, tricaine methanesulfonate, ketamine, tobacco) on hematological parameters and stress indicators of rainbow trout Oncorhynchus mykiss, Walbaum, 1792

Authors: Maryam Mohammadi, Hossein Khara

Published in: Comparative Clinical Pathology | Issue 5/2015

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Abstract

The aim of this study was to assess the efficiency of four anesthetic agents, tricaine methanesulfonate (MS-222), clove oil, 7 ketamine, and tobacco extract on juvenile rainbow trout. Also, changes of blood indices were evaluated at optimum doses of four anesthetic agents. Basal effective concentrations determined were 40 mg L−1 (induction, 111 ± 16 s and recovery time, 246 ± 36 s) for clove oil, 150 mg L−1 (induction, 287 ± 59 and recovery time, 358 ± 75 s) for MS-222, 1 mg L−1 (induction, 178 ± 38 and recovery time, 264 ± 57 s) for ketamine, and 30 mg L−1 (induction, 134 ± 22 and recovery time, 285 ± 42 s) for tobacco. According to our results, significant changes in hematological parameters including white blood cells (WBCs), red blood cells (RBCs), hematocrit (Ht), and hemoglobin (Hb) were found between four anesthetics agents. Also, significant differences were observed in some plasma parameters including cortical, glucose, and lactate between experimental treatments. Induction and recovery times for juvenile Oncorhynchus mykiss anesthetized with anesthetic agents were dose-dependent.
Literature
go back to reference Burka JF, Hammell KL, Horsberg TE, Johnson GR, Rainnie DJ, Speare DJ (1997) Drugs in Salmonid aquaculture—a review. J Vet Pharm Therap 20:333–349CrossRef Burka JF, Hammell KL, Horsberg TE, Johnson GR, Rainnie DJ, Speare DJ (1997) Drugs in Salmonid aquaculture—a review. J Vet Pharm Therap 20:333–349CrossRef
go back to reference Di Marco P, Petochi T, Finoia MG, Longobardi A, Massari A, Mandich A, Marino G (2007) Effect of anaesthetics on welfare and stress physiology in Sea Bass Dicentrarchus labrax. Aquaculture Europe 337–338 PP Di Marco P, Petochi T, Finoia MG, Longobardi A, Massari A, Mandich A, Marino G (2007) Effect of anaesthetics on welfare and stress physiology in Sea Bass Dicentrarchus labrax. Aquaculture Europe 337–338 PP
go back to reference Feng G, Zhuang P, Zhang L, Kynard B, Shi X, Liu J, Huang X (2011) Effect of anaesthetics MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii). J Appl Ichthyol 27:595–599CrossRef Feng G, Zhuang P, Zhang L, Kynard B, Shi X, Liu J, Huang X (2011) Effect of anaesthetics MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii). J Appl Ichthyol 27:595–599CrossRef
go back to reference Gullian M, Villanueva J (2009) Efficacy of tricaine methanesulphonate and clove oil as anaesthetics for juvenile cobia Rachycentron canadum. Aquac Res 40:852–860CrossRef Gullian M, Villanueva J (2009) Efficacy of tricaine methanesulphonate and clove oil as anaesthetics for juvenile cobia Rachycentron canadum. Aquac Res 40:852–860CrossRef
go back to reference Haney DC, Hursh DA, Mix MC, Winton JR (1992) Physiological and haematological changes in chum salmon artificially infected with erythrocytic necrosis virus. J Aqua Anil Hea 4:48–57CrossRef Haney DC, Hursh DA, Mix MC, Winton JR (1992) Physiological and haematological changes in chum salmon artificially infected with erythrocytic necrosis virus. J Aqua Anil Hea 4:48–57CrossRef
go back to reference Heo GJ, Shin G (2010) Efficacy of benzocaine as an anaesthetic for crucian carp (Carassius carassius). Vet Anaesth Analg 37:132–135CrossRefPubMed Heo GJ, Shin G (2010) Efficacy of benzocaine as an anaesthetic for crucian carp (Carassius carassius). Vet Anaesth Analg 37:132–135CrossRefPubMed
go back to reference Holloway AC, Keene JL, Noakes DG, Moccia RD (2004) Effects of clove oil and MS-222 on blood hormone profiles in rainbow trout Oncorhynchus mykiss, Walbaum. Aquac Res 35:1025–1030CrossRef Holloway AC, Keene JL, Noakes DG, Moccia RD (2004) Effects of clove oil and MS-222 on blood hormone profiles in rainbow trout Oncorhynchus mykiss, Walbaum. Aquac Res 35:1025–1030CrossRef
go back to reference Hseu JR, Yeh SL, Chu YT, Ting YY (1998) Comparison of efficacy of five anaesthetic goldlined sea bream, Sparus sarba. Acta Zoologica Taiwanica 9:35–41 Hseu JR, Yeh SL, Chu YT, Ting YY (1998) Comparison of efficacy of five anaesthetic goldlined sea bream, Sparus sarba. Acta Zoologica Taiwanica 9:35–41
go back to reference Iversen M, Eliassen RA, Finstad B (2009) Potential benefit of clove oil sedation on animal welfare during salmon smolt, Salmo salar L. transport and transfer to sea. Aquac Res 40:233–241CrossRef Iversen M, Eliassen RA, Finstad B (2009) Potential benefit of clove oil sedation on animal welfare during salmon smolt, Salmo salar L. transport and transfer to sea. Aquac Res 40:233–241CrossRef
go back to reference Iverzen M, Finstad B, McKinley RS, Eliassen RA (2003) The efficacy of metomidate, clove oil, Agui-Sand Benzoak as anaesthetics in Atlantic salmon stress-reducing capacity. Aquaculture 221:549–566CrossRef Iverzen M, Finstad B, McKinley RS, Eliassen RA (2003) The efficacy of metomidate, clove oil, Agui-Sand Benzoak as anaesthetics in Atlantic salmon stress-reducing capacity. Aquaculture 221:549–566CrossRef
go back to reference Jolly DW, Mawdesley-Thomas LE, Bucke D (1972) Anesthesia of fish. Vet Res 91:424–426 Jolly DW, Mawdesley-Thomas LE, Bucke D (1972) Anesthesia of fish. Vet Res 91:424–426
go back to reference King WV, Hooper B, Hillsgrove S, Benton C, Berlinsky DL (2005) The use of clove oil, metomidate, tricaine methanesulphonate and 2-phenoxyethanol for inducing anaesthesia and their effect on the cortisol stress response in black sea bass (Centropristis striata L.). Aquac Res 36:1442–1449CrossRef King WV, Hooper B, Hillsgrove S, Benton C, Berlinsky DL (2005) The use of clove oil, metomidate, tricaine methanesulphonate and 2-phenoxyethanol for inducing anaesthesia and their effect on the cortisol stress response in black sea bass (Centropristis striata L.). Aquac Res 36:1442–1449CrossRef
go back to reference Klontz GW (1994) Fish hematology. In: Stolen JS, Fletcher TC, Rowley AF, Kelikoff TC, Kaattari SL, Smith SA (Eds) Techniques in Fish Immunology, 3, SOS Publications pp. 121–132 Klontz GW (1994) Fish hematology. In: Stolen JS, Fletcher TC, Rowley AF, Kelikoff TC, Kaattari SL, Smith SA (Eds) Techniques in Fish Immunology, 3, SOS Publications pp. 121–132
go back to reference Marking LL, Meyer FP (1985) Are better anesthetics needed in fisheries? Fisheries 10:2–5CrossRef Marking LL, Meyer FP (1985) Are better anesthetics needed in fisheries? Fisheries 10:2–5CrossRef
go back to reference Mylonas CC, Cardinaletti G, Sigelaki I, Polzonetti- Magni A (2005) Comparative efficacy of clove oil and2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) at different temperatures. Aquaculture 246:467–481CrossRef Mylonas CC, Cardinaletti G, Sigelaki I, Polzonetti- Magni A (2005) Comparative efficacy of clove oil and2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) at different temperatures. Aquaculture 246:467–481CrossRef
go back to reference Pawar HB, Sanaye SV, Sreepada RA, Harish V, Suryavanshi U, Ansari ZA (2011) Comparative efficacy of four anaesthetic agents in the yellow seahorse, Hippocampus kuda (Bleeker, 1852). Aquaculture 311:155–161CrossRef Pawar HB, Sanaye SV, Sreepada RA, Harish V, Suryavanshi U, Ansari ZA (2011) Comparative efficacy of four anaesthetic agents in the yellow seahorse, Hippocampus kuda (Bleeker, 1852). Aquaculture 311:155–161CrossRef
go back to reference Pirhonen J, Schreck CB (2003) Effects of anaesthesia with MS-222, clove oil and CO2 on feed intake and plasma cortisol in steelhead trout (Oncorhynchus mykiss). Aquaculture 220:507–514CrossRef Pirhonen J, Schreck CB (2003) Effects of anaesthesia with MS-222, clove oil and CO2 on feed intake and plasma cortisol in steelhead trout (Oncorhynchus mykiss). Aquaculture 220:507–514CrossRef
go back to reference Rehulka J (2000) Influence of astaxanthin on growth rate, condition and some blood indices of rainbow trout, Oncorhynchus mykiss. Aquaculture 190:27–47CrossRef Rehulka J (2000) Influence of astaxanthin on growth rate, condition and some blood indices of rainbow trout, Oncorhynchus mykiss. Aquaculture 190:27–47CrossRef
go back to reference Ross LG, Ross B (2008) Anaesthetic and sedative techniques for aquatic animals, 3rd edn. Blackwell Publishing, Oxford, 121–134 ppCrossRef Ross LG, Ross B (2008) Anaesthetic and sedative techniques for aquatic animals, 3rd edn. Blackwell Publishing, Oxford, 121–134 ppCrossRef
go back to reference Sladky KK, Swanson CR, Stoskopf MK, Loomis MR, Lewbart GA (2001) Comparative efficacy of tricaine methanesulphonate and clove oil for use as anaesthetics in red pacu Piaractus brachypomus. Am J Vet Res 62:337–342CrossRefPubMed Sladky KK, Swanson CR, Stoskopf MK, Loomis MR, Lewbart GA (2001) Comparative efficacy of tricaine methanesulphonate and clove oil for use as anaesthetics in red pacu Piaractus brachypomus. Am J Vet Res 62:337–342CrossRefPubMed
go back to reference Small BC (2003) Anesthetic efficacy of metomidate and comparison of plasma cortisol responses to tricaine methanesulfonate, quinaldine and clove oil anesthetized channel catfish Ictalurus punctatus. Aquaculture 218:177–185CrossRef Small BC (2003) Anesthetic efficacy of metomidate and comparison of plasma cortisol responses to tricaine methanesulfonate, quinaldine and clove oil anesthetized channel catfish Ictalurus punctatus. Aquaculture 218:177–185CrossRef
go back to reference Treves-Brown KM (2000) Anaesthesia. In: Treves-Brown KM (ed) Anaesthetics in applied fish pharmacology. Kluwer Academic Publishers, Dordrecht, 206–217 ppCrossRef Treves-Brown KM (2000) Anaesthesia. In: Treves-Brown KM (ed) Anaesthetics in applied fish pharmacology. Kluwer Academic Publishers, Dordrecht, 206–217 ppCrossRef
go back to reference Velisek J, Svobodova Z, Piackova V (2005a) Effect of clove oil anaesthesia on rainbow trout (Oncorhynchus mykiss). Acta Vet Brno 74:139–146CrossRef Velisek J, Svobodova Z, Piackova V (2005a) Effect of clove oil anaesthesia on rainbow trout (Oncorhynchus mykiss). Acta Vet Brno 74:139–146CrossRef
go back to reference Velisek J, Svobodova Z, Piackova V, Groch L, Nepejchalova L (2005b) Effects of clove oil anaesthesia on common carp (Cyprinus carpio). Vet Med Czech 50:269–275 Velisek J, Svobodova Z, Piackova V, Groch L, Nepejchalova L (2005b) Effects of clove oil anaesthesia on common carp (Cyprinus carpio). Vet Med Czech 50:269–275
go back to reference Velisek J, Stara A, Li ZH, Silovska S, Turek J (2011) Comparison of effects of four anaesthetics on blood chemical profiles and oxidative stress biomarkers in rainbow trout. Aquaculture 310:369–375CrossRef Velisek J, Stara A, Li ZH, Silovska S, Turek J (2011) Comparison of effects of four anaesthetics on blood chemical profiles and oxidative stress biomarkers in rainbow trout. Aquaculture 310:369–375CrossRef
go back to reference Weber RA, Peleterio JB, Garcia Martin LO, Aldegunde M (2009) The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS-222 as anaesthetic agents in the Senegalese sole (Solea senegalensis Kaup, 1858). Aquaculture 288:147–150CrossRef Weber RA, Peleterio JB, Garcia Martin LO, Aldegunde M (2009) The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS-222 as anaesthetic agents in the Senegalese sole (Solea senegalensis Kaup, 1858). Aquaculture 288:147–150CrossRef
go back to reference Williams TD, Christiansen J, and Nygren S (1988) Intermuscular anesthesia of teleost and elasmobranchs using ketamine hydrochloride. Proc. West. Reg. Am. Assoc. Zool. Pk. Aqu. Monteray Bay Aquarium, Monteray, CA 132-l 35 pp Williams TD, Christiansen J, and Nygren S (1988) Intermuscular anesthesia of teleost and elasmobranchs using ketamine hydrochloride. Proc. West. Reg. Am. Assoc. Zool. Pk. Aqu. Monteray Bay Aquarium, Monteray, CA 132-l 35 pp
go back to reference Yamamoto Y, Woody CA, Shoji T, Ueda H (2008) Olfactory nerve response of masu salmon (Oncorhynchus masou Brevoot) and rainbow trout (O. mykiss Walbaum) to clove oil and MS-222. Aquac Res 39:1019–1027CrossRef Yamamoto Y, Woody CA, Shoji T, Ueda H (2008) Olfactory nerve response of masu salmon (Oncorhynchus masou Brevoot) and rainbow trout (O. mykiss Walbaum) to clove oil and MS-222. Aquac Res 39:1019–1027CrossRef
go back to reference Yoshikawa H, Ishida Y, Ueno S, Mitsuda H (1988) Changes in depth of anesthesia of the carp anaesthetized with a constant level of carbon dioxide. Bull Jap Soc Sci Fish 54:457–462CrossRef Yoshikawa H, Ishida Y, Ueno S, Mitsuda H (1988) Changes in depth of anesthesia of the carp anaesthetized with a constant level of carbon dioxide. Bull Jap Soc Sci Fish 54:457–462CrossRef
go back to reference Zar JH (1999) Bio-statistical analysis, 4th edn. Prentice Hall, New Jersey, 326–329 pp Zar JH (1999) Bio-statistical analysis, 4th edn. Prentice Hall, New Jersey, 326–329 pp
Metadata
Title
Effect of different anesthetic agents (clove oil, tricaine methanesulfonate, ketamine, tobacco) on hematological parameters and stress indicators of rainbow trout Oncorhynchus mykiss, Walbaum, 1792
Authors
Maryam Mohammadi
Hossein Khara
Publication date
01-09-2015
Publisher
Springer London
Published in
Comparative Clinical Pathology / Issue 5/2015
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-014-2027-2

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