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Open Access 01-03-2009 | Original Article

Immunohistochemical localization and mRNA expression of matrix Gla protein and fetuin-A in bone biopsies of hemodialysis patients

Authors: Giorgio Coen, Paola Ballanti, Giuliana Silvestrini, Daniela Mantella, Micaela Manni, Salvatore Di Giulio, Stefania Pisanò, Martina Leopizzi, Giuseppe Di Lullo, Ermanno Bonucci

Published in: Virchows Archiv | Issue 3/2009

Abstract

Matrix Gla protein (MGP) and fetuin-A are inhibitors of arterial calcifications. In blood of rats, calcium–phosphate–fetuin–MGP complexes, produced in bone, have been identified. Indeed, an association between bone resorption, release of such complexes, and arterial calcifications has been reported. We have investigated the synthesis and localization of fetuin-A and MGP in bone of hemodialysis patients and the possible contribution of bone cells in arterial calcifications. Bone biopsies from 11 hemodialysis patients were used for histology, in situ hybridization of fetuin-A and MGP messenger RNA (mRNA), immunohistochemistry of fetuin-A, and total, carboxylated, and non-carboxylated MGP proteins. Patients showed various types of renal osteodystrophy, or normal bone. MGP was synthesized and expressed (total and carboxylated) by osteoblasts, osteocytes, and most osteoclasts, while fetuin-A by osteoblasts and osteocytes. Fetuin-A and carboxylated MGP proteins were positive in the calcified matrix, while total MGP was negative. Osteoid seams were negative to fetuin-A, lightly positive to carboxylated MGP, and occasionally positive to total MGP. Undercarboxylated MGP was mostly undetectable. In adult humans, fetuin-A is produced also by osteoblasts, and not only by hepatocytes, as previously believed. MGP, essentially carboxylated, is synthesized by osteoblasts and most osteoclasts. Increased bone turnover can be an important contributor to arterial calcifications.

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Title: Immunohistochemical localization and mRNA expression of matrix Gla protein and fetuin-A in bone biopsies of hemodialysis patients
Authors: Giorgio Coen
Paola Ballanti
Giuliana Silvestrini
Daniela Mantella
Micaela Manni
Salvatore Di Giulio
Stefania Pisanò
Martina Leopizzi
Giuseppe Di Lullo
Ermanno Bonucci
Publication date: 01-03-2009
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 3/2009
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-008-0724-4

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Immunohistochemical localization and mRNA expression of matrix Gla protein and fetuin-A in bone biopsies of hemodialysis patients

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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