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01-11-2018 | Original Article

Differences in non-enzymatic glycation products in human dentine and clavicle: changes with aging

Authors: Aurora Valenzuela, Eduardo Guerra-Hernández, José Ángel Rufián-Henares, Ana Belén Márquez-Ruiz, Hans Petter Hougen, Belén García-Villanova

Published in: International Journal of Legal Medicine | Issue 6/2018

Abstract

In recent decades, several methods based on biochemical and molecular changes caused by aging have been proposed to improve the accuracy of forensic age estimation. The present study aimed to measure changes in furosine and pentosidine, two markers of non-enzymatic glycation of proteins (NEGs), in human dentine and clavicle with aging, and to identify possible differences between turnover rates in different mineralized tissues. Furosine and pentosidine were quantified in 32 dentine samples from living donors between 14 and 80 years of age, and in a second group of samples consisting of a tooth and a piece of clavicle collected from the same cadaver (15 individuals aged 18 to 85 years). Furosine concentration was much higher than pentosidine concentration in the same tissue, although they were strongly correlated in both dentine and bone. A close relationship between furosine and/or pentosidine content and chronological age was found in both tissues (r > 0.93). Moreover, age estimation was more accurate when furosine or pentosidine content was determined in dentine, with specificity values for the tests higher than 82% in all age groups. In clavicle, furosine concentration and pentosidine concentration were much lower (2.6-fold and 3.1-fold, respectively) than in dentine from the same individuals. In conclusion, although the results show strong correlations between chronological age and furosine or pentosidine concentrations determined in mineralized tissues, there is still a need for further research with larger data sets, including patients with diabetes.

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Title: Differences in non-enzymatic glycation products in human dentine and clavicle: changes with aging
Authors: Aurora Valenzuela
Eduardo Guerra-Hernández
José Ángel Rufián-Henares
Ana Belén Márquez-Ruiz
Hans Petter Hougen
Belén García-Villanova
Publication date: 01-11-2018
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Legal Medicine / Issue 6/2018
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-018-1908-3

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Differences in non-enzymatic glycation products in human dentine and clavicle: changes with aging

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