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Published in: International Journal of Legal Medicine 2/2007

01-03-2007 | Technical Note

Changes in colour of different human tissues as a marker of age

Authors: Alexander Pilin, František Pudil, Vladimír Bencko

Published in: International Journal of Legal Medicine | Issue 2/2007

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Abstract

This study deals with age estimation based on colour changes of human tissue from the intervertebral discs, Achilles tendon and rib cartilage. The investigated colour changes are the result of the accumulation of non-enzymatic browning products in the tissue. Samples of excised tissues were photographed with a digital camera and the pictures were evaluated using the image analysis processor Lucia G 4.11 processor. The values of the intensities of the RGB channels (MeanRed, Mean Green, MeanBlue) and parameters from the IHS system (MeanSaturation, HueTypical, HueVariation, BrightVariation and MeanBrightness) were evaluated. The results confirm that colour changes of some tissues depend on ageing and are a good tool for age estimation.
Literature
1.
go back to reference Johanson G (1971) Age determination from human teeth. Odontol Revy 22(suppl 21):1–26 Johanson G (1971) Age determination from human teeth. Odontol Revy 22(suppl 21):1–26
2.
3.
go back to reference Willems G (2001) A review of the most commonly used dental age estimation techniques. J Forensic Odontostomatol 19:9–17PubMed Willems G (2001) A review of the most commonly used dental age estimation techniques. J Forensic Odontostomatol 19:9–17PubMed
4.
go back to reference Ritz S, Schutz HW, Pepper C (1993) Postmortem estimation of age at death based on aspartic acid racemization in dentin: its applicability for root dentin. Int J Legal Med 105:289–293PubMedCrossRef Ritz S, Schutz HW, Pepper C (1993) Postmortem estimation of age at death based on aspartic acid racemization in dentin: its applicability for root dentin. Int J Legal Med 105:289–293PubMedCrossRef
5.
go back to reference Pilin A, Čabala R, Pudil F, Bencko V (2001) The use of the d-, l-aspartic ratio in decalcified collagen from human dentin as an estimator of human age. J Forensic Sci 46:1228–1231PubMed Pilin A, Čabala R, Pudil F, Bencko V (2001) The use of the d-, l-aspartic ratio in decalcified collagen from human dentin as an estimator of human age. J Forensic Sci 46:1228–1231PubMed
6.
go back to reference Paewinsky E, Pfeiffer H, Brinkman B (2005) Quantification of secondary dentin formation from ortopantomograms—a contribution to forensic age estimation methods in adults. Int J Legal Med 119:27–30PubMedCrossRef Paewinsky E, Pfeiffer H, Brinkman B (2005) Quantification of secondary dentin formation from ortopantomograms—a contribution to forensic age estimation methods in adults. Int J Legal Med 119:27–30PubMedCrossRef
7.
go back to reference Camariere R, Ferrante L, Cingolani M (2006) Age estimation in children by measurement of open apices in teeth. Int J Legal Med 120:49–52CrossRef Camariere R, Ferrante L, Cingolani M (2006) Age estimation in children by measurement of open apices in teeth. Int J Legal Med 120:49–52CrossRef
8.
go back to reference Iscan MY, Loth SR, Wright RK (1984) Age estimation from the rib by phase analysis: white males. J Forensic Sci 29:1094–1104PubMed Iscan MY, Loth SR, Wright RK (1984) Age estimation from the rib by phase analysis: white males. J Forensic Sci 29:1094–1104PubMed
9.
go back to reference Sinha A, Gupta V (1995) A study on estimation of age from pubic symphysis. Forensic Sci Int 75:73–78PubMedCrossRef Sinha A, Gupta V (1995) A study on estimation of age from pubic symphysis. Forensic Sci Int 75:73–78PubMedCrossRef
10.
go back to reference Pasquier E, De Saint Martin Pernot L, Burduj V, Mounayer, Le Rest C, Colin D, Mottier D, Roux C, Baccino E (1999) Determination of age at the death: assessment of an algorithm of age prediction using numerical three-dimensional CT data from pubic bones. Am J Phys Anthropol 108:261–268PubMedCrossRef Pasquier E, De Saint Martin Pernot L, Burduj V, Mounayer, Le Rest C, Colin D, Mottier D, Roux C, Baccino E (1999) Determination of age at the death: assessment of an algorithm of age prediction using numerical three-dimensional CT data from pubic bones. Am J Phys Anthropol 108:261–268PubMedCrossRef
11.
go back to reference Schulz A, Mühler M, Mutze S, Schmidt S, Reisinger W, Schmeling A (2005) Studies on the time of frame for ossification of the medial epiphysis of the clavicle as revealed by CT scans. Int J Legal Med 119:142–145PubMedCrossRef Schulz A, Mühler M, Mutze S, Schmidt S, Reisinger W, Schmeling A (2005) Studies on the time of frame for ossification of the medial epiphysis of the clavicle as revealed by CT scans. Int J Legal Med 119:142–145PubMedCrossRef
12.
go back to reference Cameriere R, Ferrante L, Mirtella D, Cingolani M (2005) Carpals and epiphyses of radius and ulna as age indicators. Int J Legal Med 120:143–146PubMedCrossRef Cameriere R, Ferrante L, Mirtella D, Cingolani M (2005) Carpals and epiphyses of radius and ulna as age indicators. Int J Legal Med 120:143–146PubMedCrossRef
13.
go back to reference Schmeling A, Schulz R, Reisinger W, Mühler M., Wenecke KD, Geserick G (2004) Studies on the time frame for ossification of the medial clavicular epiphyseal cartilage in conventional radiography. Int J Legal Med 118:5–8PubMedCrossRef Schmeling A, Schulz R, Reisinger W, Mühler M., Wenecke KD, Geserick G (2004) Studies on the time frame for ossification of the medial clavicular epiphyseal cartilage in conventional radiography. Int J Legal Med 118:5–8PubMedCrossRef
14.
go back to reference Vanezis P, Trujillo O (1996) Evaluation of hypostasis using a colorimeter measuring systém and its application to assessment of the post-mortem interval (time of death). Forensic Sci Int 78:19–28PubMedCrossRef Vanezis P, Trujillo O (1996) Evaluation of hypostasis using a colorimeter measuring systém and its application to assessment of the post-mortem interval (time of death). Forensic Sci Int 78:19–28PubMedCrossRef
15.
go back to reference Bohnert M, Weinman W, Pollak S (1999) Spectrophotometric evaluation of postmortem lividity. Forensic Sci Int 99:149–158PubMedCrossRef Bohnert M, Weinman W, Pollak S (1999) Spectrophotometric evaluation of postmortem lividity. Forensic Sci Int 99:149–158PubMedCrossRef
16.
go back to reference Trujillo O, Vanezis P, Cermignani M (1996) Photometric assessment of skin colour and lightness using a tristimulus colorimeter: reliability of inter and intra-investigator observations in healthy adult volunteers. Forensic Sci Int 81:110CrossRef Trujillo O, Vanezis P, Cermignani M (1996) Photometric assessment of skin colour and lightness using a tristimulus colorimeter: reliability of inter and intra-investigator observations in healthy adult volunteers. Forensic Sci Int 81:110CrossRef
17.
go back to reference Bohnert M, Baumgartner R, Pollak S (2000) Spectrophotometric evaluation of the colour of intra- and subcutaneous bruises. Int J Legal Med 113:343–348PubMedCrossRef Bohnert M, Baumgartner R, Pollak S (2000) Spectrophotometric evaluation of the colour of intra- and subcutaneous bruises. Int J Legal Med 113:343–348PubMedCrossRef
19.
go back to reference Ten Cate AR, Thompson GW, Dickinson JB, Hunter HA (1977) The estimation of age of skeletal remains from the colour of roots of teeth. Dent J 43:83–86PubMed Ten Cate AR, Thompson GW, Dickinson JB, Hunter HA (1977) The estimation of age of skeletal remains from the colour of roots of teeth. Dent J 43:83–86PubMed
20.
go back to reference Solheim T (1988) Dental color as an indicator of age. Gerodontics 4:114–118PubMed Solheim T (1988) Dental color as an indicator of age. Gerodontics 4:114–118PubMed
21.
go back to reference Valenzuela A, Martin De Las Heras S, Mandojana JM, De Dios Luna J, Valenzuela M, Villanueva E (2002) Multiple regression models for age estimation by assessment of morphologic dental changes according to teeth source. Am J Forensic Med Pathol 23:386–389PubMedCrossRef Valenzuela A, Martin De Las Heras S, Mandojana JM, De Dios Luna J, Valenzuela M, Villanueva E (2002) Multiple regression models for age estimation by assessment of morphologic dental changes according to teeth source. Am J Forensic Med Pathol 23:386–389PubMedCrossRef
22.
go back to reference Pudil F, Pilin A (2000) The evaluation of morphological parameters on teeth for age estimation using computer image analysis. Proceedings of “European IOFOS (International Organization of Forensic OdontoStomatology) Millennium Meeting”, Leuven, Belgium, August 23–26 Pudil F, Pilin A (2000) The evaluation of morphological parameters on teeth for age estimation using computer image analysis. Proceedings of “European IOFOS (International Organization of Forensic OdontoStomatology) Millennium Meeting”, Leuven, Belgium, August 23–26
23.
go back to reference Lackovic KP, Wood RE (2000) Tooth root colour as a measure of chronological age. J Forensic Odontostomatol 18:37–45PubMed Lackovic KP, Wood RE (2000) Tooth root colour as a measure of chronological age. J Forensic Odontostomatol 18:37–45PubMed
24.
go back to reference Hormel SE, Eyre DR (1991) Collagen in the ageing human intervertebral disc: an increase in covalently bound fluorophores and chromophores. Biochim Biophys Acta 1078:243–250PubMed Hormel SE, Eyre DR (1991) Collagen in the ageing human intervertebral disc: an increase in covalently bound fluorophores and chromophores. Biochim Biophys Acta 1078:243–250PubMed
25.
go back to reference Frye EB, Degenhardt TP, Thorpe SR, Baynes JW (1998) Role of the Maillard reaction in aging of tissue proteins. Advanced glycation end product-dependent increase in imidazolium cross-links in human lens proteins. J Biol Chem 273:18714–18719PubMedCrossRef Frye EB, Degenhardt TP, Thorpe SR, Baynes JW (1998) Role of the Maillard reaction in aging of tissue proteins. Advanced glycation end product-dependent increase in imidazolium cross-links in human lens proteins. J Biol Chem 273:18714–18719PubMedCrossRef
26.
go back to reference Bank RA, Bayliss MT, Lafeber FP, Maroudas A, Tekoppele JM (1998) Ageing and zonal variation in post-translational modification of collagen in normal human articular cartillage. The age-related increase in non-enzymatic glycation affects biochemical properties of cartilage. Biochem J 330:345–351PubMed Bank RA, Bayliss MT, Lafeber FP, Maroudas A, Tekoppele JM (1998) Ageing and zonal variation in post-translational modification of collagen in normal human articular cartillage. The age-related increase in non-enzymatic glycation affects biochemical properties of cartilage. Biochem J 330:345–351PubMed
28.
go back to reference Verzijl N, DeGroot J, Oldehinkel E, Bank RA, Thorpe SR, Baynes JW, Bayliss MT, Bijlsma J, Lafeber FP, Tekoppele JM (2000) Age related accumulation of Maillard reaction in human articular cartilage collagen. Biochem J 350:381–387PubMedCrossRef Verzijl N, DeGroot J, Oldehinkel E, Bank RA, Thorpe SR, Baynes JW, Bayliss MT, Bijlsma J, Lafeber FP, Tekoppele JM (2000) Age related accumulation of Maillard reaction in human articular cartilage collagen. Biochem J 350:381–387PubMedCrossRef
Metadata
Title
Changes in colour of different human tissues as a marker of age
Authors
Alexander Pilin
František Pudil
Vladimír Bencko
Publication date
01-03-2007
Publisher
Springer-Verlag
Published in
International Journal of Legal Medicine / Issue 2/2007
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI
https://doi.org/10.1007/s00414-006-0136-4

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