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Archives of Gynecology and Obstetrics

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01-11-2015 | Gynecologic Oncology

miR-31 functions as an oncogene in cervical cancer

Authors: Wenjing Zheng, Zhen Liu, Wei Zhang, Xiaoxia Hu

Published in: Archives of Gynecology and Obstetrics | Issue 5/2015

Abstract

Background

MicroRNAs are frequently altered in numerous cancers and are critical regulators of various diseases. miR-31 has been shown to be significantly altered in a variety of cancers.

Methods

In the present study, we measured the expression level of miR-31 in cervical cancer, CIN and normal cervical tissues by real-time RT (reverse transcription)-PCR. We also analyzed the correlations between the expression level of miR-31 and the clinical characteristics in cases of cervical squamous cell carcinoma. In addition, we measured the expression of miR-31 in cervical cancer cell lines, and transfected HPV16 E6 siRNA and HPV16 E7 siRNA into SiHa cells to investigate the effects on miR-31. Finally, the effects of miR-31 on cell proliferation, migration and invasion were measured in HeLa and SiHa cells that were transfected with a miR-31 mimic or a negative control.

Result

We found that the expression level of miR-31 was significantly higher in cervical cancer patients than in normal individuals (P < 0.05). Aberrant expression of miR-31 was positively correlated with the lymph node metastasis (LNM), vessel invasion and HPV status (P < 0.05). Additionally, miR-31 was also overexpressed in the cervical cancer-derived HeLa and SiHa cells compared with C33A cells (P < 0.05). Moreover, a relationship was found between miR-31 expression and the HPV16 oncoproteins E6/E7. Furthermore, we found that the overexpression of miR-31 can promote cell proliferation and enhance the migration and invasion abilities of cancer cells.

Conclusions

Our results suggested that miR-31 plays an oncogenetic role in the development and progression of cervical cancer.

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Title: miR-31 functions as an oncogene in cervical cancer
Authors: Wenjing Zheng
Zhen Liu
Wei Zhang
Xiaoxia Hu
Publication date: 01-11-2015
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 5/2015
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-015-3713-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

miR-31 functions as an oncogene in cervical cancer

Abstract

Background

Methods

Result

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Result

Conclusions

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