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International Journal of Colorectal Disease
Published in: International Journal of Colorectal Disease 2/2010

01-02-2010 | Original Article

Covering the colon anastomoses with amniotic membrane prevents the negative effects of early intraperitoneal 5-FU administration on anastomotic healing

Authors: Mehmet Uludag, Kursat Ozdilli, Bulent Citgez, Gurkan Yetkin, Osman M. Ipcioglu, Omer Ozcan, Nedim Polat, Abdulcabbar Kartal, Pinar Torun, Adnan Isgor

Published in: International Journal of Colorectal Disease | Issue 2/2010

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Abstract

Purpose

This study investigated whether covering the colonic anastomoses with amniotic membrane (AM) protects the anastomotic healing from the adverse effects of immediate 5-fluorouracil (5-FU) administration.

Methods

One hundred twenty wistar albino rats were randomized to one of four groups (I–IV, 30 rats in each) and underwent a standardized left colon resection and anastomoses. The anastomoses of the rats in groups II (AM) and IV (5-FU + AM) were covered with AM. Saline solution (2 ml/day; groups I (control) and II) or 5-FU (20 mg/kg/day; groups III (5-FU) and IV) was administered to the rats intraperitoneally once daily from the day of operation until sacrificed. Half of each group was sacrificed on the postoperative day 4 (IA, IIA, IIIA, and IVA) and other half on the postoperative day 8 (IB, IIB, IIIB, and IVB), and their anastomoses were evaluated when sacrificed.

Results

The dehiscence rate of anastomotic dehiscence and adhesion formation were significantly higher in groups IIIA and IIIB compared with groups IVA and IVB, respectively. Bursting pressure was significantly higher in the 5-FU + AM groups than in the 5-FU groups. The inflammatory cell infiltration was significantly lower in groups IIIA and IVA compared with group IA, in groups IIIB and IVB compared with group IB, and in group IVA compared with group IIIA. Neoangiogenesis, fibroblast activity, collagen deposition, and hydroxyproline levels were significantly higher in the 5FU + AM groups compared with control and 5-FU groups. Malondialdehyde levels were significantly higher in the 5-FU groups than in the 5-FU + AM groups.

Conclusion

Covering colon anastomoses with AM protects them, preventing leakage and reversing the negative effects of 5-FU administration.
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Metadata
Title
Covering the colon anastomoses with amniotic membrane prevents the negative effects of early intraperitoneal 5-FU administration on anastomotic healing
Authors
Mehmet Uludag
Kursat Ozdilli
Bulent Citgez
Gurkan Yetkin
Osman M. Ipcioglu
Omer Ozcan
Nedim Polat
Abdulcabbar Kartal
Pinar Torun
Adnan Isgor
Publication date
01-02-2010
Publisher
Springer-Verlag
Published in
International Journal of Colorectal Disease / Issue 2/2010
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI
https://doi.org/10.1007/s00384-009-0833-9

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