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International Journal of Colorectal Disease

Published in:

01-05-2009 | Original Article

Effects of amniotic membrane on the healing of primary colonic anastomoses in the cecal ligation and puncture model of secondary peritonitis in rats

Authors: Mehmet Uludag, Bulent Citgez, Ozay Ozkaya, Gurkan Yetkin, Omer Ozcan, Nedim Polat, Adnan Isgor

Published in: International Journal of Colorectal Disease | Issue 5/2009

Abstract

Purpose

We aimed to investigate the effects of amniotic membrane on primary colonic anastomoses in a rat peritonitis model.

Materials and methods

Fifty female Sprague Dawley rats were used in the study. Bacterial peritonitis was induced in all rats by performing a cecal ligation and puncture. Ten rats served as controls for the bursting pressure measurement, while the other 40 animals were divided into two groups (the anastomosis group (P) or the amniotic membrane group (PA)), and all of them underwent colonic anastomosis. The latter group had amniotic membrane covering their anastomoses. Half of the PA and P groups were sacrificed on the third postoperative day (PA3, P3), and the other half on the seventh postoperative day (PA7, P7).

Results

The bursting pressures were significantly higher in groups PA3 and PA7 compared with P3 (p < 0.01) and P7 (p < 0.05), respectively. Inflammatory cell infiltration and adhesion scores were significantly lower in groups PA3 and PA7 compared with groups P3 (p < 0.001, p < 0.01, respectively) and P7 (p < 0.001, p < 0.05, respectively). Neoangiogenesis, fibroblast activity, collagen deposition, and hydroxyproline concentrations were significantly higher in groups with amniotic membrane than in groups without amniotic membrane (p < 0.05, for all comparisons).

Conclusion

This study showed that the covering of colonic anastomoses with amniotic membrane significantly prevented the delaying effect of intraperitoneal sepsis and provided a safer and stronger anastomosis than suture and that this was the case for both the early and late phases of anastomotic healing in the colon.

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Title: Effects of amniotic membrane on the healing of primary colonic anastomoses in the cecal ligation and puncture model of secondary peritonitis in rats
Authors: Mehmet Uludag
Bulent Citgez
Ozay Ozkaya
Gurkan Yetkin
Omer Ozcan
Nedim Polat
Adnan Isgor
Publication date: 01-05-2009
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 5/2009
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-009-0645-y

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Effects of amniotic membrane on the healing of primary colonic anastomoses in the cecal ligation and puncture model of secondary peritonitis in rats

Abstract

Purpose

Materials and methods

Results

Conclusion

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Abstract

Purpose

Materials and methods

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