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Pediatric Surgery International

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21-10-2022 | Cholangitis | Original Article

Primary exploring the value of metagenomic next‐generation sequencing in detecting pathogenic bacteria of cholangitis with biliary atresia after Kasai operation

Authors: Pu Yu, Mengdi Li, Rongjuan Sun, Jianghua Zhan, Yongkang Pan

Published in: Pediatric Surgery International | Issue 12/2022

Abstract

Purpose

To evaluate the value of metagenomic next‐generation sequencing (mNGS) in detecting pathogenic bacteria of cholangitis for patients with biliary atresia after Kasai operation.

Methods

This study retrospectively analyzed patients of biliary atresia with cholangitis after Kasai operation who were admitted to Xi’an Children’s Hospital from July 2019 to December 2021. Both blood culture and mNGS were carried out in all of these patients. We compared the detection rate of pathogenic bacteria, pathogenic bacteria spectrum, test time, inflammatory indicators and liver function. All the patients were followed up for 0.5–3 years to evaluate the onset of cholangitis and the survival status of autologous liver.

Results

This study included total of 30 cholangitis occurred in 25 patients. There were significant differences in the detection rate of pathogenic bacteria [23.3 vs.73.3%, P < 0.05] and the test time [120 (114.5–120) vs.16 (16–21) h, P < 0.001] between the blood culture and mNGS. These two methods showed significant statistical differences in comparing inflammatory indicators (CRP, PCT) and liver function (TB, DB, GGT) before and after anti-infection. Four kinds of bacteria were detected by blood cultures and ten kinds of bacteria were detected by mNGS. Cholangitis occurred 3 times in one case (4%) and twice in three cases (12%). Autologous liver survived in 17 cases (68%).

Conclusion

Comparing with traditional blood culture, mNGS is more efficient, convenient and accurate in the detection of pathogens. It provides a new method for accurately detecting pathogenic bacteria of cholangitis after Kasai operation.

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Title: Primary exploring the value of metagenomic next‐generation sequencing in detecting pathogenic bacteria of cholangitis with biliary atresia after Kasai operation
Authors: Pu Yu
Mengdi Li
Rongjuan Sun
Jianghua Zhan
Yongkang Pan
Publication date: 21-10-2022
Publisher: Springer Berlin Heidelberg
Keyword: Cholangitis
Published in: Pediatric Surgery International / Issue 12/2022
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-022-05254-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Primary exploring the value of metagenomic next‐generation sequencing in detecting pathogenic bacteria of cholangitis with biliary atresia after Kasai operation

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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