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Pediatric Surgery International

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01-10-2017 | Original Article

The novel preventive effect of Daikenchuto (TJ-100), a Japanese herbal drug, against neonatal necrotizing enterocolitis in rats

Authors: Shin Shinyama, Tatsuru Kaji, Motoi Mukai, Kazuhiko Nakame, Hiroshi Matsufuji, Hideo Takamatsu, Satoshi Ieiri

Published in: Pediatric Surgery International | Issue 10/2017

Abstract

Purpose

Necrotizing enterocolitis (NEC) is a devastating gastrointestinal disease of premature infants. Daikenchuto, a Japanese herbal drug, has several effects on the digestive system, so we investigated its preventive effects in a rat model of NEC.

Methods

NEC was induced in newborn rats via asphyxia (100% N₂ for 90 s; every 4 h) + LPS (4 mg/kg/day [administered orally on days 0 and 1]). The effects of Daikenchuto were evaluated in four groups (control: 0 g/kg/day, I: 0.3 g/kg/day, II: 0.6 g/kg/day, and III: 1.0 g/kg/day). Daikenchuto was administered into the stomach through a microcatheter. The incidence and severity of NEC were pathologically assessed using the NEC grade in accordance with Dovorak’s previous report. Cell positivity for inflammatory cytokine (IL-6) was also evaluated.

Results

Daikenchuto reduced the incidence of NEC in control, Groups I, II, and III to 68.7, 30.0, 30.7, and 13.3%, respectively. High-dose Daikenchuto significantly improved the incidence of NEC, and the rate of IL-6 positive cells in group III was significantly lower than in the control group (p = 0.04).

Conclusion

We evaluated the effect of Daikenchuto against NEC and found that it reduced the incidence rate of NEC due to a decrease in the IL-6 production.

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Title: The novel preventive effect of Daikenchuto (TJ-100), a Japanese herbal drug, against neonatal necrotizing enterocolitis in rats
Authors: Shin Shinyama
Tatsuru Kaji
Motoi Mukai
Kazuhiko Nakame
Hiroshi Matsufuji
Hideo Takamatsu
Satoshi Ieiri
Publication date: 01-10-2017
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Surgery International / Issue 10/2017
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-017-4145-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The novel preventive effect of Daikenchuto (TJ-100), a Japanese herbal drug, against neonatal necrotizing enterocolitis in rats

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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