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01-01-2017 | Original Article

Characterization of gut microbiota profiles in coronary artery disease patients using data mining analysis of terminal restriction fragment length polymorphism: gut microbiota could be a diagnostic marker of coronary artery disease

Authors: Takuo Emoto, Tomoya Yamashita, Toshio Kobayashi, Naoto Sasaki, Yushi Hirota, Tomohiro Hayashi, Anna So, Kazuyuki Kasahara, Keiko Yodoi, Takuya Matsumoto, Taiji Mizoguchi, Wataru Ogawa, Ken-ichi Hirata

Published in: Heart and Vessels | Issue 1/2017

Abstract

The association between atherosclerosis and gut microbiota has been attracting increased attention. We previously demonstrated a possible link between gut microbiota and coronary artery disease. Our aim of this study was to clarify the gut microbiota profiles in coronary artery disease patients using data mining analysis of terminal restriction fragment length polymorphism (T-RFLP). This study included 39 coronary artery disease (CAD) patients and 30 age- and sex- matched no-CAD controls (Ctrls) with coronary risk factors. Bacterial DNA was extracted from their fecal samples and analyzed by T-RFLP and data mining analysis using the classification and regression algorithm. Five additional CAD patients were newly recruited to confirm the reliability of this analysis. Data mining analysis could divide the composition of gut microbiota into 2 characteristic nodes. The CAD group was classified into 4 CAD pattern nodes (35/39 = 90 %), while the Ctrl group was classified into 3 Ctrl pattern nodes (28/30 = 93 %). Five additional CAD samples were applied to the same dividing model, which could validate the accuracy to predict the risk of CAD by data mining analysis. We could demonstrate that operational taxonomic unit 853 (OTU853), OTU657, and OTU990 were determined important both by the data mining method and by the usual statistical comparison. We classified the gut microbiota profiles in coronary artery disease patients using data mining analysis of T-RFLP data and demonstrated the possibility that gut microbiota is a diagnostic marker of suffering from CAD.

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Title: Characterization of gut microbiota profiles in coronary artery disease patients using data mining analysis of terminal restriction fragment length polymorphism: gut microbiota could be a diagnostic marker of coronary artery disease
Authors: Takuo Emoto
Tomoya Yamashita
Toshio Kobayashi
Naoto Sasaki
Yushi Hirota
Tomohiro Hayashi
Anna So
Kazuyuki Kasahara
Keiko Yodoi
Takuya Matsumoto
Taiji Mizoguchi
Wataru Ogawa
Ken-ichi Hirata
Publication date: 01-01-2017
Publisher: Springer Japan
Published in: Heart and Vessels / Issue 1/2017
Print ISSN: 0910-8327
Electronic ISSN: 1615-2573
DOI: https://doi.org/10.1007/s00380-016-0841-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Characterization of gut microbiota profiles in coronary artery disease patients using data mining analysis of terminal restriction fragment length polymorphism: gut microbiota could be a diagnostic marker of coronary artery disease

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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