Top

Published in:

01-11-2009 | Magnetic Resonance

Acute myocarditis in a rat model: late gadolinium enhancement with histopathological correlation

Authors: Huedayi Korkusuz, Philip Esters, Nagy Naguib, N.-E. Nour Eldin, Sebastian Lindemayr, Frank Huebner, Ahmed Koujan, Reinhold Bug, Hanns Ackermann, Thomas J. Vogl

Published in: European Radiology | Issue 11/2009

Abstract

The aim of the current study was to use an established animal model of autoimmune myocarditis and to judge the ability of cardiovascular MRI (CMR) in quantitatively measuring the extent of myocardial involvement compared with histopathological measurement of severity and extent. Experimental autoimmune myocarditis (EAM) was induced in 10 male Lewis rats. On day 21, all animals were investigated by CMR to measure the extent of late gadolinium enhancement (LGE). Subsequently, histopathological evaluation of the entire heart was performed. All animals of the experimental group fulfilled histopathological criteria of myocarditis, revealing necrosis in seven of eight cases. At reduced heart rate, area of LGE correlated highly with histologically proven area of myocarditis (r = 0.80–0.87, p < 0.05). LGE was mainly located in the anterior (range 50–62.5%) and lateral (range 62.5–75%) left ventricular wall and septum (range 25–50%) with a midwall to subepicardial accentuation. The LGE pattern found by CMR can be regarded as suggestive of EAM. With cellular necrosis being the main mechanism for LGE we were able to show high correlations between CMR examination results and histopathologically proven areas of myocarditis. Thus we think the current animal model can provide the opportunity for further fundamental research into myocarditis.

Skouri HN, Dec GW, Friedrich MG et al (2006) Noninvasive imaging in myocarditis. J Am Coll Cardiol 48:2085–2093CrossRefPubMed

Neuspiel DR, Kuller LH (1985) Sudden and unexpected natural death in childhood and adolescence. JAMA 254:1321–1325CrossRefPubMed

Lie JT (1988) Myocarditis and endomyocardial biopsy in unexplained heart failure: a diagnosis in search of a disease. Ann Intern Med 109:525–528PubMed

Codreanu A, Djaballah W, Angioi M et al (2007) Detection of myocarditis by contrast-enhanced MRI in patients presenting with acute coronary syndrome but no coronary stenosis. J Magn Reson Imaging 25:957–964CrossRefPubMed

Marcu CB, Beek AM, van Rossum AC (2006) Clinical applications of cardiovascular magnetic resonance imaging. CMAJ 175:911–917PubMed

Sechtem U, Mahrholdt H, Vogelsberg H (2007) Cardiac magnetic resonance in myocardial disease. Heart (British Cardiac Society) 93:1520–1527

Yelgec NS, Dymarkowski S, Ganame J et al (2007) Value of MRI in patients with a clinical suspicion of acute myocarditis. Eur Radiol 17:2211–2217CrossRefPubMed

Stork A, Mullerleile K, Bansmann PM et al (2007) Patterns of delayed-enhancement in MRI of ischemic and non-ischemic cardiomyopathies. Rofo 179:21–30PubMed

Kodama M, Tachikawa H, Kashimura T et al (2004) Effects of humoral factors on left ventricular remodeling under chronic heart failure. Nippon yakurigaku zasshi 123:63–70PubMed

10.

Veeraveedu PT, Watanabe K, Ma M et al (2008) Torasemide, a long-acting loop diuretic, reduces the progression of myocarditis to dilated cardiomyopathy. Eur J Pharmacol 581:121–131CrossRefPubMed

11.

Yoshida Y, Shioi T, Izumi T (2007) Resveratrol ameliorates experimental autoimmune myocarditis. Circ J 71:397–404CrossRefPubMed

12.

Kodama M, Matsumoto Y, Fujiwara M et al (1990) A novel experimental model of giant cell myocarditis induced in rats by immunization with cardiac myosin fraction. Clin Immunol Immunopathol 57:250–262CrossRefPubMed

13.

Kodama M, Matsumoto Y, Fujiwara M et al (1991) Characteristics of giant cells and factors related to the formation of giant cells in myocarditis. Circ Res 69:1042–1050PubMed

14.

Kodama M, Izumi T (1991) Experimental autoimmune myocarditis. Acta Med Biol 39:1–10

15.

Davies LA, Hamilton DL, Hopkins PM et al (1999) Concentration-dependent inotropic effects of halothane, isoflurane and sevoflurane on rat ventricular myocytes. Br J Anaesth 82:723–730PubMed

16.

Gilson WD, Kraitchman DL (2007) Cardiac magnetic resonance imaging in small rodents using clinical 1.5 T and 3.0 T scanners. Methods (San Diego, Calif) 43:35–45

17.

Yuan Z, Liu Y, Liu Y et al (2004) Peroxisome proliferator-activated receptor-gamma ligands ameliorate experimental autoimmune myocarditis associated with inhibition of self-sensitive T cells. J Cardiovasc Pharmacol 43:868–875CrossRefPubMed

18.

Kanda T, Takahashi T, Kudo S et al (2004) Leptin deficiency enhances myocardial necrosis and lethality in a murine model of viral myocarditis. Life Sci 75:1435–1447CrossRefPubMed

19.

Tomlinson DR, Becher H, Selvanayagam JB (2008) Assessment of myocardial viability: comparison of echocardiography versus cardiac magnetic resonance imaging in the current era. Heart Lung Circ 17:173–185CrossRefPubMed

20.

Friedrich MG, Abdel-Aty H, Taylor A et al (2008) The salvaged area at risk in reperfused acute myocardial infarction as visualized by cardiovascular magnetic resonance. J Am Coll Cardiol 51:1581–1587CrossRefPubMed

21.

Saraste A, Nekolla S, Schwaiger M (2008) Contrast-enhanced magnetic resonance imaging in the assessment of myocardial infarction and viability. J Nucl Cardiol 15:105–117CrossRefPubMed

22.

Mahrholdt H, Wagner A, Deluigi CC et al (2006) Presentation, patterns of myocardial damage, and clinical course of viral myocarditis. Circulation 114:1581–1590CrossRefPubMed

23.

Abdel-Aty H, Boye P, Zagrosek A et al (2005) Diagnostic performance of cardiovascular magnetic resonance in patients with suspected acute myocarditis: comparison of different approaches. J Am Coll Cardiol 45:1815–1822CrossRefPubMed

24.

Uribe S, Muthurangu V, Boubertakh R et al (2007) Whole-heart cine MRI using real-time respiratory self-gating. Magn Reson Med 57:606–613CrossRefPubMed

Title: Acute myocarditis in a rat model: late gadolinium enhancement with histopathological correlation
Authors: Huedayi Korkusuz
Philip Esters
Nagy Naguib
N.-E. Nour Eldin
Sebastian Lindemayr
Frank Huebner
Ahmed Koujan
Reinhold Bug
Hanns Ackermann
Thomas J. Vogl
Publication date: 01-11-2009
Publisher: Springer-Verlag
Published in: European Radiology / Issue 11/2009
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-009-1454-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Acute myocarditis in a rat model: late gadolinium enhancement with histopathological correlation

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 11/2009

MRI findings in patients with severe trismus following radiotherapy for nasopharyngeal carcinoma

Clinical Doppler ultrasound for the assessment of plaque ulceration in the stenosed carotid bifurcation by detection of distal turbulence intensity: a matched model study

Assessment of parathyroid glands in hemodialysis patients by using color Doppler sonography

Delay before the hepatocyte phase of Gd-EOB-DTPA-enhanced MR imaging: Is it possible to shorten the examination time?

Combined use of T2-weighted and diffusion-weighted 3-T MR imaging for differentiating uterine sarcomas from benign leiomyomas

Obturator externus bursa: prevalence of communication with the hip joint and associated intra-articular findings in 200 consecutive hip MR arthrograms