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01-12-2011 | Original Article

Influence of Age and Body Mass Index on the Yield and Proliferation Capacity of Adipose-Derived Stem Cells

Authors: Ali Mojallal, Charlotte Lequeux, Christo Shipkov, Antoine Duclos, Fabienne Braye, Rod Rohrich, Spencer Brown, Odile Damour

Published in: Aesthetic Plastic Surgery | Issue 6/2011

Abstract

Background

Adipose tissue is commonly used for volume restoration. It is also a source of adipose-derived stem cells (ASCs), easy to obtain in large quantities by liposuction or resection techniques. The aim of this study was to determine the influence of body mass index (BMI) and age on the number (yield) and proliferation capacity of ASCs.

Methods

A prospective study was conducted in 42 women. They were divided into two groups: age ≤ 40 or >40 and BMI ≤ 25 or >25. Fat tissue was harvested via manual lipoaspiration always from the abdominal region. After centrifugation in the OR, the harvested fat (100 cc) was sent to the laboratory for isolation and cultivation of ASCs. The yield of viable ASCs was evaluated by the trypan blue exclusion test. Viable ASCs were cultured and their proliferation capacity was evaluated by the growth kinetics assay. Results were statistically analyzed.

Results

The average cell yield was 0.380 × 10⁶/ml. Cell yield and proliferation capacity did not show statistically significant correlation to the age and BMI of patients, with regression lines showing null correlation. There was no significant difference between the cell yield and proliferation capacity between the different groups.

Conclusion

The results from this study suggest that there is no statistically significant correlation between ASC yield and proliferation capacity and age and BMI.

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Title: Influence of Age and Body Mass Index on the Yield and Proliferation Capacity of Adipose-Derived Stem Cells
Authors: Ali Mojallal
Charlotte Lequeux
Christo Shipkov
Antoine Duclos
Fabienne Braye
Rod Rohrich
Spencer Brown
Odile Damour
Publication date: 01-12-2011
Publisher: Springer-Verlag
Published in: Aesthetic Plastic Surgery / Issue 6/2011
Print ISSN: 0364-216X
Electronic ISSN: 1432-5241
DOI: https://doi.org/10.1007/s00266-011-9743-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Influence of Age and Body Mass Index on the Yield and Proliferation Capacity of Adipose-Derived Stem Cells

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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