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European Journal of Nuclear Medicine and Molecular Imaging

Published in:

01-02-2008 | Original Article

⁶⁸Ga-DOTAVAP-P1 PET imaging capable of demonstrating the phase of inflammation in healing bones and the progress of infection in osteomyelitic bones

Authors: Petteri Lankinen, Tatu J. Mäkinen, Tiina A. Pöyhönen, Pauliina Virsu, Satu Salomäki, Antti J. Hakanen, Sirpa Jalkanen, Hannu T. Aro, Anne Roivainen

Published in: European Journal of Nuclear Medicine and Molecular Imaging | Issue 2/2008

Abstract

Purpose

Differentiation between bacterial infection and nonbacterial inflammation remains a diagnostic challenge. Vascular adhesion protein 1 (VAP-1) is a human endothelial protein whose cell surface expression is induced under inflammatory conditions, thus making it a highly promising target molecule for studying inflammatory processes in vivo. We hypothesized that positron emission tomography (PET) with gallium-68-labeled 1,4,7,10-tetraazacyclododecane-N′,N″,N′′′,N″″-tetraacetic acid-peptide targeted to VAP-1 (⁶⁸Ga-DOTAVAP-P1) could be feasible for imaging the early inflammatory and infectious processes in healing bones.

Materials and methods

Thirty-four Sprague–Dawley rats with diffuse Staphylococcus aureus tibial osteomyelitis and 34 rats with healing cortical bone defects (representing the inflammation stage of healing) were PET imaged using ⁶⁸Ga-DOTAVAP-P1 as a tracer. In addition, peripheral quantitative computed tomography and conventional radiography were performed. Bone samples for quantitative bacteriology and specimens were also processed for histomorphometry of inflammatory and infectious reactions.

Results

PET imaging showed an uptake of ⁶⁸Ga-DOTAVAP-P1 in both the osteomyelitic bones and the healing cortical bone defects during the first 36 h after surgery. Thereafter, only the osteomyelitic tibias were delineated by PET. The osteomyelitic and control animals showed a similar uptake of the ⁶⁸Ga-DOTAVAP-P1 at 24 h, whereas a significant difference was observed at 7 days (p < 0.0001).

Conclusions

The current study showed that PET imaging with the new ⁶⁸Ga-DOTAVAP-P1 is capable of accurately demonstrating the phase of inflammation in healing bones and the progress of bacterial infection in osteomyelitic bones. Consequently, this novel imaging agent allowed for the differentiation of bone infection due to S. aureus and normal bone healing as soon as 7 days after onset.

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Title: 68Ga-DOTAVAP-P1 PET imaging capable of demonstrating the phase of inflammation in healing bones and the progress of infection in osteomyelitic bones
Authors: Petteri Lankinen
Tatu J. Mäkinen
Tiina A. Pöyhönen
Pauliina Virsu
Satu Salomäki
Antti J. Hakanen
Sirpa Jalkanen
Hannu T. Aro
Anne Roivainen
Publication date: 01-02-2008
Publisher: Springer-Verlag
Published in: European Journal of Nuclear Medicine and Molecular Imaging / Issue 2/2008
Print ISSN: 1619-7070
Electronic ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-007-0637-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

⁶⁸Ga-DOTAVAP-P1 PET imaging capable of demonstrating the phase of inflammation in healing bones and the progress of infection in osteomyelitic bones

Abstract

Purpose

Materials and methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Materials and methods

Results

Conclusions

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