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European Journal of Nuclear Medicine and Molecular Imaging

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01-10-2006 | Original article

Labelling of human mesenchymal stem cells with indium-111 for SPECT imaging: effect on cell proliferation and differentiation

Authors: L. Bindslev, M. Haack-Sørensen, K. Bisgaard, L. Kragh, S. Mortensen, B. Hesse, A. Kjær, J. Kastrup

Published in: European Journal of Nuclear Medicine and Molecular Imaging | Issue 10/2006

Abstract

Purpose

Stem cell therapy seems to be a new treatment option within cardiac diseases to improve myocardial perfusion and function. However, the delivery and traceability of the cells represent a problem. Radioactive labelling with ¹¹¹In could be a method for tracking mesenchymal stem cells (MSCs). However, ¹¹¹In could influence the viability and differentiation capacity of MSCs, which would limit its use. Therefore, the aim of this study was to evaluate the influence of ¹¹¹In labelling in doses relevant for SPECT imaging in humans on the viability and differentiation capacity of human MSCs.

Methods and results

Human MSCs isolated from bone marrow were incubated with ¹¹¹In-tropolone (15–800 Bq/cell). The labelling efficiency was approximately 25% with 30 Bq/cell ¹¹¹In. The MSC doubling time was 1.04±0.1 days and was not influenced by ¹¹¹In within the range 15–260 Bq/cell. Using 30 Bq ¹¹¹In/cell it was possible to label MSCs to a level relevant for clinical scintigraphic use. With this dose, ¹¹¹In had no effect on characteristic surface and intracellular markers of cultured MSCs analysed both by flow cytometry and by real-time polymerase chain reaction. Further, the labelled MSCs differentiated towards endothelial cells and formed vascular structures.

Conclusion

It is possible to label human MSCs with ¹¹¹In for scintigraphic tracking of stem cells delivered to the heart in clinical trials without affecting the viability and differentiation capacity of the MSCs. This creates an important tool for the control of stem cell delivery and dose response in clinical cardiovascular trials.

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Title: Labelling of human mesenchymal stem cells with indium-111 for SPECT imaging: effect on cell proliferation and differentiation
Authors: L. Bindslev
M. Haack-Sørensen
K. Bisgaard
L. Kragh
S. Mortensen
B. Hesse
A. Kjær
J. Kastrup
Publication date: 01-10-2006
Publisher: Springer-Verlag
Published in: European Journal of Nuclear Medicine and Molecular Imaging / Issue 10/2006
Print ISSN: 1619-7070
Electronic ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-006-0093-7

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Springer Medicine

Labelling of human mesenchymal stem cells with indium-111 for SPECT imaging: effect on cell proliferation and differentiation

Abstract

Purpose

Methods and results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods and results

Conclusion

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