Published in:
01-01-2006 | Brief Report
Arginine vasopressin does not alter mucosal tissue oxygen tension and oxygen supply in an acute endotoxemic pig model
Published in: Intensive Care Medicine | Issue 1/2006
Login to get accessAbstract
Objective
To determine the effects of increasing dosages of continuously infused arginine-vasopressin (AVP) on mucosal tissue oxygen tension and oxygen supply in an auto-perfused, innervated jejunal segment in an acute endotoxic porcine model.
Design
Prospective, randomized, experimental study.
Setting
University hospital animal research laboratory.
Interventions
Jejunal mucosal tissue PO2 was measured employing two Clark-type surface oxygen electrodes. Oxygen saturation of jejunal microvascular hemoglobin was determined by tissue reflectance spectrophotometry. Systemic hemodynamic variables, mesenteric-venous and systemic acid base and blood gas variables and lactate measurements were recorded. Measurements were performed at baseline, after E. coli lipopolysaccharide (LPS) administration and at 20 min intervals during incremental AVP infusion ( n =8; 0.014, 0.029, 0.057, 0.114 and 0.229 IU kg-1 h-1, respectively) or infusion of saline ( n =8).
Measurements and results
LPS infusion leads to a significant ( P <0.05) decrease of mucosal tissue oxygen tension (PO2muc, 24±3 to 12±2 mmHg) and microvascular hemoglobin oxygen saturation (HbO2, 38±4 to 21±4%). Mesenteric venous lactate level increased (2.4±0.3 to 4.7±1.7 mmol l-1), while mesenteric venous pH decreased (7.38±0.02 to 7.26±0.12), indicating tissue hypoxia. AVP significantly increased mean arterial pressure (MAP, 81±15 to 97±17 at 0.057 IU kg-1 h-1). No differences in jejunal mucosal oxygenation occurred between study groups at any dosage during the experimental protocol.
Conclusion
AVP administration did not further compromise mucosal tissue oxygen tension and oxygen supply in the acute phase of endotoxic pigs.