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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

Published in:

01-11-2013 | Original article

In vivo differentiation of human periodontal ligament cells leads to formation of dental hard tissue

Authors: Dr. M. Wolf, S. Lossdörfer, N. Abuduwali, R. Meyer, S. Kebir, W. Götz, A. Jäger

Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Issue 6/2013

Abstract

Objective

Following trauma, periodontal disease, or orthodontic tooth movement, residual periodontal ligament (PDL) cells at the defect site are considered mandatory for successful regeneration of the injured structures. Recent developments in tissue engineering focus, as one pillar, on the transplantation of PDL cells to support periodontal regeneration processes. Here, we examined the ability of osteogenically predifferentiated PDL cells to undergo further osteoblastic or cementoblastic differentiation and to mineralize their extracellular matrix when transplanted in an in vivo microenvironment.

Materials and methods

Using collagen sponges as carriers, osteogenically predifferentiated human PDL cells were transplanted subcutaneously into six immunocompromised CD-1® nude mice. Following explantation after 28 days, osteogenic and cementogenic marker protein expression was visualized immunohistochemically.

Results

After 28 days, transplanted PDL cells revealed both cellular, cytoplasmatic and extracellular immunoreactivity for the chosen markers alkaline phosphatase, osteopontin, PTH-receptor 1, and osteocalcin. Specific osteogenic and cementoblastic differentiation was demonstrated by RUNX2 and CEMP1 immunoreactivity. Early stages of mineralization were demonstrated by calcium and phosphate staining.

Conclusion

Our results reinforce the previously published reports of PDL cell mineralization in vivo and further demonstrate the successful induction of specific osteogenic and cementogenic differentiation of transplanted human PDL cells in vivo. These findings reveal promising possibilities for supporting periodontal remodeling and regeneration processes with PDL cells being potential target cells with which to influence the process of orthodontically induced root resorption.

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Title: In vivo differentiation of human periodontal ligament cells leads to formation of dental hard tissue
Authors: Dr. M. Wolf
S. Lossdörfer
N. Abuduwali
R. Meyer
S. Kebir
W. Götz
A. Jäger
Publication date: 01-11-2013
Publisher: Springer Berlin Heidelberg
Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Issue 6/2013
Print ISSN: 1434-5293
Electronic ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-013-0155-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

In vivo differentiation of human periodontal ligament cells leads to formation of dental hard tissue

Abstract

Objective

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Materials and methods

Results

Conclusion

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