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Inflammation Research
Published in: Inflammation Research 1/2011

01-01-2011 | Original Research Paper

IgE-dependent sensitization increases responsiveness to LPS but does not modify development of endotoxin tolerance in mast cells

Authors: Jaciel Medina-Tamayo, Alfredo Ibarra-Sánchez, Alejandro Padilla-Trejo, Claudia González-Espinosa

Published in: Inflammation Research | Issue 1/2011

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Abstract

Objective

Effects of immunoglobulin E (IgE)-dependent sensitization on the response to bacterial lipopolysaccharide (LPS) were analyzed in mast cells.

Methods

Murine bone marrow-derived mast cells (BMMCs) were sensitized or not with IgE before stimulation with LPS. TLR4 and co-receptors expression was analyzed by flow cytometry and RT-PCR, TNF-α production by ELISA, IKK and IκB activation by western blot or immunoprecipitation. NFκB nuclear translocation was determined by EMSA.

Results

IgE-sensitized BMMCs secreted larger amounts of TNF-α than non-sensitized cells shortly after LPS challenge. No change in TLR4, CD14 or MD-2 expression was detected after the IgE-dependent sensitization process, whereas TLR4-dependent phosphorylation of IKK and IκB was augmented. IgE-dependent sensitization increased basal NFκB activity. Endotoxin tolerance was not affected by the IgE-dependent sensitization process.

Conclusions

IgE-induced sensitization primes mast cells for higher response to LPS through pre-activation of NFκB transcription factor. IgE-dependent sensitization does not modify events leading to endotoxin tolerance.
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Metadata
Title
IgE-dependent sensitization increases responsiveness to LPS but does not modify development of endotoxin tolerance in mast cells
Authors
Jaciel Medina-Tamayo
Alfredo Ibarra-Sánchez
Alejandro Padilla-Trejo
Claudia González-Espinosa
Publication date
01-01-2011
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 1/2011
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-010-0230-4

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