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Inflammation Research
Published in: Inflammation Research 12/2009

01-12-2009 | Original Research Paper

Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production

Authors: Maud M. Gueders, Genevieve Paulissen, Celine Crahay, Florence Quesada-Calvo, Jonathan Hacha, Chris Van Hove, Kurt Tournoy, Renaud Louis, Jean-Michel Foidart, Agnes Noël, Didier D. Cataldo

Published in: Inflammation Research | Issue 12/2009

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Abstract

Objective

Animal models of asthma mimic major features of human disease. Since the genetic background of experimental animals might affect hyperresponsiveness and inflammation, we studied its potential influence and the mechanisms leading to differences in strains.

Methods

We applied a mouse model of allergic asthma to BALB/c and C57BL/6 mice.

Results

BALB/c mice displayed greater levels of airway reactivity to methacholine than C57BL/6 mice. Moreover, BALB/c mice exhibited higher numbers of mast cells in lung tissue when compared to C57BL/6. On the contrary, eosinophil and neutrophil counts in bronchoalveolar lavage fluid (BALF) as well as peribronchial eosinophilia were greater in C57BL/6. IL (Interleukin)-4, IL-5, IL-13, and CCL11 levels measured in whole-lung extracts were higher in BALB/c, while, in sharp contrast, CCL11 and CCL5 levels were higher in BALF of C57BL/6 mice.

Conclusions

We observed phenotypic differences between C57BL/6 and BALB/c mice in an asthma model with different distributions of pro-inflammatory cytokines and inflammatory cells.
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Metadata
Title
Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production
Authors
Maud M. Gueders
Genevieve Paulissen
Celine Crahay
Florence Quesada-Calvo
Jonathan Hacha
Chris Van Hove
Kurt Tournoy
Renaud Louis
Jean-Michel Foidart
Agnes Noël
Didier D. Cataldo
Publication date
01-12-2009
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 12/2009
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-009-0054-2

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