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Inflammation Research
Published in: Inflammation Research 9/2009

01-09-2009 | Original Research Paper

Expansion of CD14+CD16+ peripheral monocytes among patients with aseptic loosening

Authors: W. Wu, X. Zhang, C. Zhang, T. Tang, W. Ren, K. Dai

Published in: Inflammation Research | Issue 9/2009

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Abstract

Objective and design

In this study, we have investigated the relevance of peripheral blood inflammatory CD14+CD16+ monocytes phenotype to patients with aseptic loosening (AL).

Material and treatment

Immunophenotypes of monocytes were examined among patients with AL (n = 43), patients with mechanical loosening (ML, n = 30), patients with stable implant (SI, n = 16), and patients with osteoarthritis (OA, n = 17) using flow cytometry.

Methods

Immunological assay was used to measure TNF-α and IL-1β levels in both sera and culture media of implant wear stimulated CD14+CD16+ and CD14++CD16− monocytes. Periprosthetic tissues were collected during surgery for histological assessment.

Results

The frequency of CD14+CD16+ monocytes showed significant increase in AL patients than in ML, SI, and OA patients. A positive association was found between the subpopulation of CD14+CD16+ monocytes and plasma TNF-α and IL-1β level in AL patients. Furthermore, a positive correlation existed between the subpopulation of CD14+CD16+ monocytes and the total histopathology score.

Conclusion

The results indicate that CD14+CD16+ monocytes represent a sensitive marker for the disease activity of AL, and may serve as an effective prognostic index to identify total joint replacement recipients who are at increased risk for osteolysis and progression of AL.
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Metadata
Title
Expansion of CD14+CD16+ peripheral monocytes among patients with aseptic loosening
Authors
W. Wu
X. Zhang
C. Zhang
T. Tang
W. Ren
K. Dai
Publication date
01-09-2009
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 9/2009
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-009-0020-z

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