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01-05-2013

Limited replication of influenza A virus in human mast cells

Authors: Candy W. Marcet, Chris D. St. Laurent, Tae Chul Moon, Nav Singh, A. Dean Befus

Published in: Immunologic Research | Issue 1/2013

Abstract

Mast cells are important in innate immunity and protective against certain bacterial infections. However, there is limited evidence that mast cells respond to viruses. As mast cells are abundant in mucosal tissues of the lung, they are in a prime location to detect and respond to influenza virus. In this study, we characterized for the first time the replication cycle of influenza A virus in human mast cells by measuring influenza A virus transcription, RNA replication, protein synthesis, and formation of infectious virus as compared to the replication cycle in epithelial cells. We detected the presence of influenza A viral genomic RNA transcription, replication, and protein synthesis in human mast cells and epithelial cells. However, there was no significant release of infectious influenza A virus from mast cells, whereas epithelial cells produce ~100-fold virus compared with the inoculating dose. We confirmed that influenza A virus infects human mast cells, begins to replicate, but the production of new virus is aborted. Thus, mast cells may lack critical factors essential for productive infection or there are intrinsic or inducible anti-influenza A mechanisms in mast cells.

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Title: Limited replication of influenza A virus in human mast cells
Authors: Candy W. Marcet
Chris D. St. Laurent
Tae Chul Moon
Nav Singh
A. Dean Befus
Publication date: 01-05-2013
Publisher: Springer-Verlag
Published in: Immunologic Research / Issue 1/2013
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI: https://doi.org/10.1007/s12026-012-8377-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Limited replication of influenza A virus in human mast cells

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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