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Immunologic Research
Published in: Immunologic Research 1/2013

01-05-2013

Adjuvant dependence of APS pathology-related low-affinity antibodies during secondary immune response to tetanus toxoid in BALB/c mice

Authors: Irena Živković, Vladimir Petrušić, Rajna Dimitrijević, Marijana Stojanović, Ljiljana Dimitrijević

Published in: Immunologic Research | Issue 1/2013

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Abstract

One of the established animal models for autoimmune disease antiphospholipid syndrome (APS) is TTd hyperimmunization of mice. Tetanus toxoid (TTd) and plasma protein β2GPI share structural homology so that immunization with TTd induces appearance of cross-reactive antibodies. In this paper, we have investigated the presence and dynamic of fluctuation of specific (anti-TTd) and auto (anti-β2GPI) antibodies induced in BALB/c mice during secondary immune response after TTd immunization with alhydrogel or glycerol as adjuvants. In addition, we followed the induced reproductive pathology as a sign of autoimmune outcome. We show undoubtedly adjuvant dependance of (1) level of induced anti-TTd IgG antibodies, (2) changes in levels of low-affinity anti-β2GPI IgG antibodies, and (3) change in fecundity and fertility during secondary immune response. These findings once more indicate the importance of chosen adjuvants used for successful immunization and eventual autoantibody outcome, this time associated with the processes involving low affinity, natural antibodies.
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Metadata
Title
Adjuvant dependence of APS pathology-related low-affinity antibodies during secondary immune response to tetanus toxoid in BALB/c mice
Authors
Irena Živković
Vladimir Petrušić
Rajna Dimitrijević
Marijana Stojanović
Ljiljana Dimitrijević
Publication date
01-05-2013
Publisher
Springer-Verlag
Published in
Immunologic Research / Issue 1/2013
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI
https://doi.org/10.1007/s12026-012-8378-3

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